PMID- 26208647
OWN - NLM
STAT- MEDLINE
DCOM- 20160812
LR  - 20220318
IS  - 1460-2431 (Electronic)
IS  - 0022-0957 (Print)
IS  - 0022-0957 (Linking)
VI  - 66
IP  - 21
DP  - 2015 Nov
TI  - Spatial distribution of centromeres and telomeres at interphase varies among 
      Brachypodium species.
PG  - 6623-34
LID - 10.1093/jxb/erv369 [doi]
AB  - In this study the 3-D distribution of centromeres and telomeres was analysed in 
      the interphase nuclei of three Brachypodium species, i.e. B. distachyon (2n=10), 
      B. stacei (2n=20) and B. hybridum (2n=30), which is presumably a hybrid between 
      the first two species. Using fluorescence in situ hybridization (FISH) with 
      centromeric and telomeric DNA probes, it was observed that the majority of B. 
      distachyon nuclei in the root tip cells displayed the Rabl configuration while 
      both B. stacei and B. hybridum mostly lacked the centromere-telomere 
      polarization. In addition, differentiated leaf cells of B. distachyon did not 
      display the Rabl pattern. In order to analyse the possible connection between the 
      occurrence of the Rabl pattern and the phase of cell cycle or DNA content, FISH 
      was combined with digital image cytometry. The results revealed that the 
      frequency of nuclei with the Rabl configuration in the root tip nuclei was 
      positively correlated with an increase in DNA content, which resulted from DNA 
      replication. Also, the analysis of the influence of the nuclear shape on the 
      nuclear architecture indicated that an increasing elongation of the nuclei 
      negatively affected the occurrence of the Rabl pattern. Some possible 
      explanations of these phenomena are discussed.
CI  - (c) The Author 2015. Published by Oxford University Press on behalf of the Society 
      for Experimental Biology.
FAU - Idziak, Dominika
AU  - Idziak D
AD  - Department of Plant Anatomy and Cytology, Faculty of Biology and Environmental 
      Protection, University of Silesia in Katowice, 28 Jagiellonska Street, 40-032 
      Katowice, Poland dominika.idziak@us.edu.pl.
FAU - Robaszkiewicz, Ewa
AU  - Robaszkiewicz E
AD  - Department of Plant Anatomy and Cytology, Faculty of Biology and Environmental 
      Protection, University of Silesia in Katowice, 28 Jagiellonska Street, 40-032 
      Katowice, Poland.
FAU - Hasterok, Robert
AU  - Hasterok R
AD  - Department of Plant Anatomy and Cytology, Faculty of Biology and Environmental 
      Protection, University of Silesia in Katowice, 28 Jagiellonska Street, 40-032 
      Katowice, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150724
PL  - England
TA  - J Exp Bot
JT  - Journal of experimental botany
JID - 9882906
SB  - IM
MH  - Brachypodium/*genetics
MH  - Centromere/*genetics
MH  - *Chromosomes, Plant
MH  - Genome, Plant
MH  - Meristem
MH  - Species Specificity
MH  - Telomere/*genetics
PMC - PMC4623680
OTO - NOTNLM
OT  - Brachypodium
OT  - Rabl configuration
OT  - centromeres
OT  - interphase
OT  - model grass
OT  - molecular cytogenetics
OT  - telomeres.
EDAT- 2015/07/26 06:00
MHDA- 2016/08/16 06:00
PMCR- 2015/07/24
CRDT- 2015/07/26 06:00
PHST- 2015/07/26 06:00 [entrez]
PHST- 2015/07/26 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
PHST- 2015/07/24 00:00 [pmc-release]
AID - erv369 [pii]
AID - 10.1093/jxb/erv369 [doi]
PST - ppublish
SO  - J Exp Bot. 2015 Nov;66(21):6623-34. doi: 10.1093/jxb/erv369. Epub 2015 Jul 24.