PMID- 26208751
OWN - NLM
STAT- MEDLINE
DCOM- 20160714
LR  - 20220311
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 46
IP  - 2
DP  - 2015 Oct
TI  - Cytokine effects and cellular signaling pathways of grass carp HSP70 in head 
      kidney leukocytes.
PG  - 550-6
LID - S1050-4648(15)30079-6 [pii]
LID - 10.1016/j.fsi.2015.07.016 [doi]
AB  - In mammals, the cytokine effects of heat shock protein 70 (HSP70) have been well 
      documented. However, the precise role and molecular mechanism of fish HSP70 in 
      cytokine production are poorly understood. In the present study, recombinant 
      grass carp HSP70 (rgcHSP70) was prepared by using the E. coli expression system 
      and its effect on interleukin (IL)-1beta (gcil1beta) and tumor necrosis factor (TNF)-alpha 
      (gctnfalpha) mRNA expression was examined in grass carp head kidney leukocytes 
      (HKLs). Compared with its heated-inactivated protein, rgcHSP70 enhanced the mRNA 
      levels of these two cytokines in a dose- and time-dependent manner. More 
      importantly, the results of enzyme-linked immunoabsorbent assay (ELISA) showed 
      that rgcHSP70 markedly stimulated gcIL-1beta and gcTNF-alpha secretion from the HKLs, 
      for the first time indicating the ability of HSP70 to regulate cytokine release 
      in fish. Moreover, rgcHSP70 displayed more potential to increase the cytokines' 
      release instead of their transcription in the same cell model. Subsequently, the 
      signaling mechanisms governing the cytokine effect of rgcHSP70 were investigated, 
      showing that rgcHSP70-induced gcil1beta mRNA expression was suppressed by inhibiting 
      p38 MAPK and NF-kappaB pathways, while the stimulation of rgcHSP70 on gctnfalpha mRNA 
      expression were blocked by inhibiting JNK MAPK and NF-kappaB pathways. In parallel, 
      rgcHSP70 was also shown to activate NF-kappaB pathway through regulating grass carp 
      IkappaBalpha protein levels in a time-dependent oscillation. These findings provide 
      further understanding of cytokine effect and regulatory mechanism of HSP70 in 
      fish and new insights into the roles of HSP70 in teleost immunity.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Zhang, Anying
AU  - Zhang A
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, PR China.
FAU - Guo, Yafei
AU  - Guo Y
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, PR China.
FAU - Zhang, Shengnan
AU  - Zhang S
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, PR China.
FAU - Fan, Xinchi
AU  - Fan X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, PR China.
FAU - Wang, Xinyan
AU  - Wang X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, PR China.
FAU - Zhou, Xiaofei
AU  - Zhou X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, PR China.
FAU - Yang, Kun
AU  - Yang K
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, PR China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, PR China. Electronic address: 
      zhouhongzh@uestc.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150722
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Cytokines)
RN  - 0 (Fish Proteins)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - *Adaptive Immunity
MH  - Animals
MH  - Carps/*genetics/immunology/metabolism
MH  - Cytokines/metabolism
MH  - Escherichia coli/genetics
MH  - Fish Proteins/*genetics/metabolism
MH  - HSP70 Heat-Shock Proteins/*genetics/metabolism
MH  - Head Kidney/immunology
MH  - *Immunity, Innate
MH  - Leukocytes/immunology
MH  - NF-kappa B/genetics/metabolism
MH  - Organisms, Genetically Modified/genetics
MH  - Recombinant Proteins/genetics/metabolism
MH  - Signal Transduction
OTO - NOTNLM
OT  - Cytokine effect
OT  - Cytokine secretion
OT  - Grass carp
OT  - Head kidney leukocyte
OT  - Heat shock protein 70
OT  - Signaling pathway
EDAT- 2015/07/26 06:00
MHDA- 2016/07/15 06:00
CRDT- 2015/07/26 06:00
PHST- 2015/04/16 00:00 [received]
PHST- 2015/07/07 00:00 [revised]
PHST- 2015/07/19 00:00 [accepted]
PHST- 2015/07/26 06:00 [entrez]
PHST- 2015/07/26 06:00 [pubmed]
PHST- 2016/07/15 06:00 [medline]
AID - S1050-4648(15)30079-6 [pii]
AID - 10.1016/j.fsi.2015.07.016 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2015 Oct;46(2):550-6. doi: 10.1016/j.fsi.2015.07.016. 
      Epub 2015 Jul 22.