PMID- 26209441
OWN - NLM
STAT- MEDLINE
DCOM- 20160226
LR  - 20181113
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 12
DP  - 2015 Jul 25
TI  - Complete genome sequence of Ostreid herpesvirus-1 associated with mortalities of 
      Scapharca broughtonii broodstocks.
PG  - 110
LID - 10.1186/s12985-015-0334-0 [doi]
LID - 110
AB  - BACKGROUND: Ostreid herpesvirus-1 (OsHV-1) is the major bivalve pathogen 
      associated with severe mortality events in a wide host range. In the early summer 
      of 2012 and 2013, mass mortalities of blood clam (Scapharca broughtonii) 
      broodstocks associated with a newly described variant of OsHV-1 (OsHV-1-SB) were 
      reported. METHODS: In this study, the complete genome sequence of the newly 
      described variant was determined through the primer walking approach, and 
      compared with those of the other two OsHV-1 variants. RESULTS: OsHV-1-SB genome 
      was found to contain 199, 354 bp nucleotides with 38.5% G/C content, which is 
      highly similar to those of acute viral necrosis virus (AVNV) and OsHV-1 reference 
      type. A total of 123 open reading frames (ORFs) putatively encoding functional 
      proteins were identified; eight of which were duplicated in the major repeat 
      elements of the genome. The genomic organization of OsHV-1-SB could be 
      represented as TRL-UL-IRL-IRS-US-TRS, which is different from that of OsHV-1 
      reference type and AVNV due to the deletion of a unique region (X, 1.5Kb) between 
      IRL and IRS. The DNA sequence of OsHV-1-SB is 95.2% and 97.3% identical to that 
      of OsHV-1 reference type and AVNV respectively. On the basis of nucleotide 
      sequences of 32 ORFs in OsHV-1-SB and the other nine OsHV-1 variants, results 
      from phylogenetic analysis also demonstrated that OsHV-1-SB is most closely 
      related to AVNV. CONCLUSIONS: The determination of the genome of OsHV-1 with 
      distinguished epidemiological features will aid in our better understanding of 
      OsHV-1 diversity, and facilitate further research on the origin, evolution, and 
      epidemiology of the virus.
FAU - Xia, Junyang
AU  - Xia J
AD  - College of Marine Life Sciences, Ocean University of China, Qingdao, China. 
      xjy_junyang@126.com.
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao, China. xjy_junyang@126.com.
FAU - Bai, Changming
AU  - Bai C
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao, China. baicm@ysfri.ac.cn.
AD  - Function Laboratory for Marine Fisheries Science and Food Production Processes, 
      Qingdao National Oceanography Laboratory, Qingdao, China. baicm@ysfri.ac.cn.
FAU - Wang, Chongming
AU  - Wang C
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao, China. wangcm@ysfri.ac.cn.
AD  - Function Laboratory for Marine Fisheries Science and Food Production Processes, 
      Qingdao National Oceanography Laboratory, Qingdao, China. wangcm@ysfri.ac.cn.
FAU - Song, Xiaoling
AU  - Song X
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao, China. songxl@ysfri.ac.cn.
AD  - Function Laboratory for Marine Fisheries Science and Food Production Processes, 
      Qingdao National Oceanography Laboratory, Qingdao, China. songxl@ysfri.ac.cn.
FAU - Huang, Jie
AU  - Huang J
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao, China. huangjie@ysfri.ac.cn.
AD  - Function Laboratory for Marine Fisheries Science and Food Production Processes, 
      Qingdao National Oceanography Laboratory, Qingdao, China. huangjie@ysfri.ac.cn.
LA  - eng
SI  - GENBANK/KP412538
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150725
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Cluster Analysis
MH  - DNA, Viral/*chemistry/genetics
MH  - Gene Order
MH  - *Genome, Viral
MH  - Herpesviridae/classification/*genetics/*isolation & purification
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Scapharca/*virology
MH  - *Sequence Analysis, DNA
MH  - Sequence Homology
MH  - Viral Proteins/genetics
PMC - PMC4514999
EDAT- 2015/07/26 06:00
MHDA- 2016/02/27 06:00
PMCR- 2015/07/25
CRDT- 2015/07/26 06:00
PHST- 2015/04/19 00:00 [received]
PHST- 2015/06/30 00:00 [accepted]
PHST- 2015/07/26 06:00 [entrez]
PHST- 2015/07/26 06:00 [pubmed]
PHST- 2016/02/27 06:00 [medline]
PHST- 2015/07/25 00:00 [pmc-release]
AID - 10.1186/s12985-015-0334-0 [pii]
AID - 334 [pii]
AID - 10.1186/s12985-015-0334-0 [doi]
PST - epublish
SO  - Virol J. 2015 Jul 25;12:110. doi: 10.1186/s12985-015-0334-0.