PMID- 26209890
OWN - NLM
STAT- MEDLINE
DCOM- 20160425
LR  - 20211203
IS  - 1876-7753 (Electronic)
IS  - 1873-5061 (Linking)
VI  - 15
IP  - 1
DP  - 2015 Jul
TI  - Neuro-peptide treatment with Cerebrolysin improves the survival of neural stem 
      cell grafts in an APP transgenic model of Alzheimer disease.
PG  - 54-67
LID - S1873-5061(15)00050-1 [pii]
LID - 10.1016/j.scr.2015.04.008 [doi]
AB  - Neural stem cells (NSCs) have been considered as potential therapy in Alzheimer's 
      disease (AD) but their use is hampered by the poor survival of grafted cells. 
      Supply of neurotrophic factors to the grafted cells has been proposed as a way to 
      augment survival of the stem cells. In this context, we investigated the utility 
      of Cerebrolysin (CBL), a peptidergic mixture with neurotrophic-like properties, 
      as an adjunct to stem cell therapy in an APP transgenic (tg) model of AD. We 
      grafted murine NSCs into the hippocampus of non-tg and APP tg that were treated 
      systemically with CBL and analyzed after 1, 3, 6 and 9months post grafting. 
      Compared to vehicle-treated non-tg mice, in the vehicle-treated APP tg mice there 
      was considerable reduction in the survival of the grafted NSCs. Whereas, CBL 
      treatment enhanced the survival of NSCs in both non-tg and APP tg with the 
      majority of the surviving NSCs remaining as neuroblasts. The NSCs of the CBL 
      treated mice displayed reduced numbers of caspase-3 and TUNEL positive cells and 
      increased brain derived neurotrophic factor (BDNF) and furin immunoreactivity. 
      These results suggest that CBL might protect grafted NSCs and as such be a 
      potential adjuvant therapy when combined with grafting.
CI  - Copyright (c) 2015. Published by Elsevier B.V.
FAU - Rockenstein, Edward
AU  - Rockenstein E
AD  - Department of Neurosciences, University of California San Diego, La Jolla, CA, 
      USA.
FAU - Desplats, Paula
AU  - Desplats P
AD  - Department of Neurosciences, University of California San Diego, La Jolla, CA, 
      USA.
FAU - Ubhi, Kiren
AU  - Ubhi K
AD  - Department of Neurosciences, University of California San Diego, La Jolla, CA, 
      USA.
FAU - Mante, Michael
AU  - Mante M
AD  - Department of Neurosciences, University of California San Diego, La Jolla, CA, 
      USA.
FAU - Florio, Jazmin
AU  - Florio J
AD  - Department of Neurosciences, University of California San Diego, La Jolla, CA, 
      USA.
FAU - Adame, Anthony
AU  - Adame A
AD  - Department of Neurosciences, University of California San Diego, La Jolla, CA, 
      USA.
FAU - Winter, Stefan
AU  - Winter S
AD  - Clinical Research & Pharmacology, EVER Neuro Pharma GmbH, Unterach, Austria.
FAU - Brandstaetter, Hemma
AU  - Brandstaetter H
AD  - Clinical Research & Pharmacology, EVER Neuro Pharma GmbH, Unterach, Austria.
FAU - Meier, Dieter
AU  - Meier D
AD  - Clinical Research & Pharmacology, EVER Neuro Pharma GmbH, Unterach, Austria.
FAU - Masliah, Eliezer
AU  - Masliah E
AD  - Department of Neurosciences, University of California San Diego, La Jolla, CA, 
      USA; Department of Pathology, University of California San Diego, La Jolla, CA, 
      USA. Electronic address: emasliah@ucsd.edu.
LA  - eng
GR  - AG05131/AG/NIA NIH HHS/United States
GR  - AG18440/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150509
PL  - England
TA  - Stem Cell Res
JT  - Stem cell research
JID - 101316957
RN  - 0 (Amino Acids)
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Doublecortin Domain Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Mtap2 protein, mouse)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuropeptides)
RN  - 37KZM6S21G (cerebrolysin)
RN  - EC 3.4.21.75 (Furin)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Alzheimer Disease/pathology/*therapy
MH  - Amino Acids/pharmacology/*therapeutic use
MH  - Amyloid beta-Peptides/*genetics
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Disease Models, Animal
MH  - Doublecortin Domain Proteins
MH  - Furin/metabolism
MH  - Hippocampus/drug effects/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Mice, Transgenic
MH  - Microtubule-Associated Proteins/metabolism
MH  - Neoplasms/pathology
MH  - Nerve Growth Factors/metabolism
MH  - Neural Stem Cells/*cytology/drug effects/*transplantation
MH  - Neurogenesis/drug effects
MH  - Neuropeptides/metabolism/pharmacology/*therapeutic use
EDAT- 2015/07/27 06:00
MHDA- 2016/04/26 06:00
CRDT- 2015/07/27 06:00
PHST- 2014/12/23 00:00 [received]
PHST- 2015/03/31 00:00 [revised]
PHST- 2015/04/30 00:00 [accepted]
PHST- 2015/07/27 06:00 [entrez]
PHST- 2015/07/27 06:00 [pubmed]
PHST- 2016/04/26 06:00 [medline]
AID - S1873-5061(15)00050-1 [pii]
AID - 10.1016/j.scr.2015.04.008 [doi]
PST - ppublish
SO  - Stem Cell Res. 2015 Jul;15(1):54-67. doi: 10.1016/j.scr.2015.04.008. Epub 2015 
      May 9.