PMID- 26210180
OWN - NLM
STAT- MEDLINE
DCOM- 20160523
LR  - 20211203
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 67
DP  - 2015 Oct
TI  - Regulation of vascular smooth muscle cell autophagy by DNA nanotube-conjugated 
      mTOR siRNA.
PG  - 137-50
LID - S0142-9612(15)00591-8 [pii]
LID - 10.1016/j.biomaterials.2015.07.015 [doi]
AB  - The efficient delivery of short interfering RNA (siRNA) is an enormous challenge 
      in the field of gene therapy. Herein, we report a delivery nanosystem based on 
      programmed DNA self-assembly mammalian target of rapamycin (mTOR) siRNA-loaded 
      DNA nanotubes (DNA-NTs). We demonstrate that these siRNA-DNA-NTs can be 
      effectively transfected into pulmonary arterial smooth muscle cells (PASMCs) via 
      endocytosis; and that the loaded mTOR siRNA can induce obvious autophagy and 
      inhibit cell growth under both normal and hypoxic conditions. Moreover, we found 
      that mTOR siRNA can control the autophagy and proliferation of PASMCs under 
      hypoxic condition, suggesting a potential therapeutic application for mTOR siRNA 
      in diseases involving abnormal autophagy in PASMCs.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - You, Zaichun
AU  - You Z
AD  - Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical 
      University, Chongqing 400037, China; Department of Emergency, Xinqiao Hospital, 
      Chongqing 400037, China.
FAU - Qian, Hang
AU  - Qian H
AD  - State Key Lab of Physical Chemistry of Solid Surfaces, and Department of 
      Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, 
      Xiamen 361005, China.
FAU - Wang, Changzheng
AU  - Wang C
AD  - Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical 
      University, Chongqing 400037, China.
FAU - He, Binfeng
AU  - He B
AD  - Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical 
      University, Chongqing 400037, China.
FAU - Yan, Jiawei
AU  - Yan J
AD  - State Key Lab of Physical Chemistry of Solid Surfaces, and Department of 
      Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, 
      Xiamen 361005, China.
FAU - Mao, Chengde
AU  - Mao C
AD  - Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA. 
      Electronic address: mao@purdue.edu.
FAU - Wang, Guansong
AU  - Wang G
AD  - Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical 
      University, Chongqing 400037, China. Electronic address: wanggs2003@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150716
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RNA, Small Interfering)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - *Autophagy/drug effects
MH  - Cell Death/drug effects
MH  - Cell Hypoxia/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - DNA/*metabolism
MH  - Endocytosis/drug effects
MH  - Flow Cytometry
MH  - Microtubule-Associated Proteins/metabolism
MH  - Muscle, Smooth, Vascular/*cytology
MH  - Myocytes, Smooth Muscle/*cytology/ultrastructure
MH  - Nanotubes/*chemistry/toxicity/ultrastructure
MH  - Phagosomes/drug effects/metabolism
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - RNA, Small Interfering/*metabolism
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Transfection
OTO - NOTNLM
OT  - Anoxia
OT  - Autophagy
OT  - DNA nanostructures
OT  - DNA nanotubes
OT  - Self-assembly
OT  - mTOR
OT  - siRNA
EDAT- 2015/07/27 06:00
MHDA- 2016/05/24 06:00
CRDT- 2015/07/27 06:00
PHST- 2015/06/19 00:00 [received]
PHST- 2015/07/05 00:00 [revised]
PHST- 2015/07/10 00:00 [accepted]
PHST- 2015/07/27 06:00 [entrez]
PHST- 2015/07/27 06:00 [pubmed]
PHST- 2016/05/24 06:00 [medline]
AID - S0142-9612(15)00591-8 [pii]
AID - 10.1016/j.biomaterials.2015.07.015 [doi]
PST - ppublish
SO  - Biomaterials. 2015 Oct;67:137-50. doi: 10.1016/j.biomaterials.2015.07.015. Epub 
      2015 Jul 16.