PMID- 26215374
OWN - NLM
STAT- MEDLINE
DCOM- 20160602
LR  - 20220410
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 220
IP  - 11
DP  - 2015 Nov
TI  - Bone marrow-derived macrophages exclusively expressed caveolin-2: The role of 
      inflammatory activators and hypoxia.
PG  - 1266-74
LID - S0171-2985(15)30019-X [pii]
LID - 10.1016/j.imbio.2015.06.018 [doi]
AB  - Caveolins are specific proteins involved in regulation of signal transduction to 
      intracellular space. Still, their contribution to immune functions has not been 
      completely clarified. Thus, we decided to characterize the expression of 
      caveolins in bone marrow-derived macrophages (BMDMs) under resting and 
      inflammatory conditions. The effect of classical activators (lipopolysaccharide, 
      LPS; interferon-gamma, IFN-gamma) was further potentiated with hypoxic (5% O2) 
      conditions. The activation of p44/42-extracellular signal-regulated kinases 1 and 
      2 (ERK1/2) and expression of caveolin-1, -2, and -3, hypoxia inducible factor-1 
      alpha (HIF-1alpha), as well as inducible nitric oxide synthase (iNOS) was monitored 
      using the Western blot technique. The production of nitric oxide (NO) and tumor 
      necrosis factor-alpha (TNFalpha) was analyzed by Griess method or ELISA, 
      respectively. BMDMs were also transfected with siRNA against caveolin-2. 
      Importantly, our study showed for the first time that BMDMs expressed only 
      caveolin-2, and its level decreased after activation of macrophages with LPS, 
      IFN-gamma, and/or hypoxia. The expression of caveolin-2 negatively correlates with 
      the iNOS and HIF-1alpha protein levels, as well as with the LPS/IFN-gamma- and 
      hypoxia-induced activation of ERK1/2. We concluded that caveolin-2 is most 
      probably involved in regulation of pro-inflammatory responses of BMDMs, triggered 
      via activation of ERK1/2.
CI  - Copyright (c) 2015 Elsevier GmbH. All rights reserved.
FAU - Maceckova, Michaela
AU  - Maceckova M
AD  - Department of Animal Physiology and Immunology, Institute of Experimental 
      Biology, Faculty of Science, Masaryk University, Kotlarska 267/2, 611 37 Brno, 
      Czech Republic; Institute of Biophysics, Academy of Sciences of the Czech 
      Republic, Kralovopolska 135, 612 65 Brno, Czech Republic.
FAU - Martiskova, Hana
AU  - Martiskova H
AD  - Department of Animal Physiology and Immunology, Institute of Experimental 
      Biology, Faculty of Science, Masaryk University, Kotlarska 267/2, 611 37 Brno, 
      Czech Republic; Institute of Biophysics, Academy of Sciences of the Czech 
      Republic, Kralovopolska 135, 612 65 Brno, Czech Republic.
FAU - Koudelka, Adolf
AU  - Koudelka A
AD  - Department of Animal Physiology and Immunology, Institute of Experimental 
      Biology, Faculty of Science, Masaryk University, Kotlarska 267/2, 611 37 Brno, 
      Czech Republic; Institute of Biophysics, Academy of Sciences of the Czech 
      Republic, Kralovopolska 135, 612 65 Brno, Czech Republic.
FAU - Kubala, Lukas
AU  - Kubala L
AD  - Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 
      135, 612 65 Brno, Czech Republic.
FAU - Lojek, Antonin
AU  - Lojek A
AD  - Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 
      135, 612 65 Brno, Czech Republic.
FAU - Pekarova, Michaela
AU  - Pekarova M
AD  - Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 
      135, 612 65 Brno, Czech Republic. Electronic address: pekarovam@ibp.cz.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150709
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Caveolin 2)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Small Interfering)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Animals
MH  - Caveolin 2/*genetics/*metabolism
MH  - Cell Survival
MH  - Cytokines/metabolism
MH  - *Gene Expression
MH  - Gene Expression Regulation
MH  - Hypoxia/metabolism
MH  - Inflammation/genetics/immunology/metabolism
MH  - Macrophage Activation/genetics/immunology
MH  - Macrophages/*immunology/*metabolism
MH  - Male
MH  - Mice
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type II/genetics/metabolism
MH  - Phenotype
MH  - RNA Interference
MH  - RNA, Small Interfering/genetics
OTO - NOTNLM
OT  - Caveolin-2
OT  - Hypoxia
OT  - Inflammation
OT  - Interferon-gamma
OT  - Lipopolysaccharide
OT  - Macrophage
EDAT- 2015/07/29 06:00
MHDA- 2016/06/03 06:00
CRDT- 2015/07/29 06:00
PHST- 2015/02/04 00:00 [received]
PHST- 2015/06/17 00:00 [revised]
PHST- 2015/06/30 00:00 [accepted]
PHST- 2015/07/29 06:00 [entrez]
PHST- 2015/07/29 06:00 [pubmed]
PHST- 2016/06/03 06:00 [medline]
AID - S0171-2985(15)30019-X [pii]
AID - 10.1016/j.imbio.2015.06.018 [doi]
PST - ppublish
SO  - Immunobiology. 2015 Nov;220(11):1266-74. doi: 10.1016/j.imbio.2015.06.018. Epub 
      2015 Jul 9.