PMID- 26218525
OWN - NLM
STAT- MEDLINE
DCOM- 20160629
LR  - 20150915
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 134
DP  - 2015 Oct 1
TI  - Stable surface coating of silicone elastomer with phosphorylcholine and 
      organosilane copolymer with cross-linking for repelling proteins.
PG  - 384-91
LID - S0927-7765(15)30074-6 [pii]
LID - 10.1016/j.colsurfb.2015.07.040 [doi]
AB  - Poly(dimethylsiloxane) (PDMS)-based polymers are widely used in blood-contacting 
      medical devices. However, the hydrophobic property causes adsorption of plasma 
      proteins and activates blood clotting. There are several techniques for PDMS 
      surface modification. However, the efficacy is limited to short duration due to 
      the low glass transition temperature of PDMS. The goal of this study is to 
      fabricate a highly stable polymer membrane with blood-compatibility on PDMS. 
      Here, we synthesized random copolymer of 2-methacryloyloxyethyl phosphorylcholine 
      (MPC), 3-methacryloxypropyl trimethoxysilane (MPTMSi) and 3-(methacryloyloxy) 
      propyl-tris(trimethylsilyloxy) silane (MPTSSi). These copolymers are covalently 
      and physically immobilized on PDMS surface by silane coupling (cross-linking) and 
      hydrophobic interaction. Protein adsorption was significantly reduced on MPC 
      copolymer-coated PDMS surface. In particular, copolymer containing 50% MPC unit 
      was the most effective and maintained the effect for the longest duration (84 
      days). From analyses of X-ray reflectometry (XRR) and X-ray photoelectron 
      spectroscopy (XPS), it is determined that the density of the polymer membrane is 
      an important factor for the long-term stability. In addition, the coating of PDMS 
      with MPC copolymer does not influence on oxygen permeability.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Nagahashi, Koji
AU  - Nagahashi K
AD  - Department of Bioengineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, 
      Tokyo 113-8656, Japan.
FAU - Teramura, Yuji
AU  - Teramura Y
AD  - Department of Bioengineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, 
      Tokyo 113-8656, Japan.
FAU - Takai, Madoka
AU  - Takai M
AD  - Department of Bioengineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, 
      Tokyo 113-8656, Japan. Electronic address: takai@bis.t.u-tokyo.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150718
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Elastomers)
RN  - 0 (Polymers)
RN  - 0 (Proteins)
RN  - 0 (Silicones)
RN  - 107-73-3 (Phosphorylcholine)
SB  - IM
MH  - *Coated Materials, Biocompatible
MH  - *Elastomers
MH  - Microscopy, Fluorescence
MH  - Phosphorylcholine/*chemistry
MH  - Polymers/*chemistry
MH  - Proteins/*chemistry
MH  - Silicones/*chemistry
MH  - Surface Properties
OTO - NOTNLM
OT  - 2-Methacryloyloxyethylphosphorylcholine (MPC)
OT  - Blood compatibility
OT  - Copolymer
OT  - Polydimethylsiloxane (PDMS)
OT  - Surface modification
EDAT- 2015/07/29 06:00
MHDA- 2016/06/30 06:00
CRDT- 2015/07/29 06:00
PHST- 2015/04/01 00:00 [received]
PHST- 2015/06/29 00:00 [revised]
PHST- 2015/07/16 00:00 [accepted]
PHST- 2015/07/29 06:00 [entrez]
PHST- 2015/07/29 06:00 [pubmed]
PHST- 2016/06/30 06:00 [medline]
AID - S0927-7765(15)30074-6 [pii]
AID - 10.1016/j.colsurfb.2015.07.040 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2015 Oct 1;134:384-91. doi: 
      10.1016/j.colsurfb.2015.07.040. Epub 2015 Jul 18.