PMID- 26218899
OWN - NLM
STAT- MEDLINE
DCOM- 20161005
LR  - 20200409
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 33
IP  - 51
DP  - 2015 Dec 16
TI  - Successful cross-protective efficacy induced by heat-adapted live attenuated 
      nephropathogenic infectious bronchitis virus derived from a natural recombinant 
      strain.
PG  - 7370-7374
LID - S0264-410X(15)01002-6 [pii]
LID - 10.1016/j.vaccine.2015.07.043 [doi]
AB  - A natural recombinant nephropathogenic K40/09 strain of infectious bronchitis 
      virus (IBV) was heat-adapted for possible future use as live attenuated vaccine. 
      The K40/09 strain was selected during successive serial passages in 
      specific-pathogen free (SPF) embryonated eggs at sub-optimal higher temperature 
      (56 degrees C). Unlike the parental strain, the attenuated strain, designated K40/09 
      HP50, was found to be safe in 1-day-old SPF chicks, which showed neither 
      mortality nor signs of morbidity, and rarely induced ciliostasis or histological 
      changes in the trachea and kidney after intraocular and fine-spray 
      administration. K40/09 HP50 provided almost complete protection against two 
      distinct subgroups of a nephropathogenic strain (KM91-like and QX-like subgroup) 
      and elicited the production of high titers of neutralizing antibody 
      (neutralization index of 3.6). We conclude that the K40/09 HP50 vaccine virus is 
      rapidly attenuated by heat adaptation and exhibits the desired level of 
      attenuation, immunogenicity, and protective efficacy required for a live 
      attenuated vaccine. These results indicate that the K40/09 vaccine could be 
      helpful for the reduction of economic losses caused by recently emergent 
      nephropathogenic IBV infection in many countries.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Lim, Tae-Hyun
AU  - Lim TH
AD  - Optipharm Inc., Osongsangmyung 6ro, Osong-eup, CheongJu, Chungcheongbuk-do, 
      Republic of Korea.
FAU - Youn, Ha-Na
AU  - Youn HN
AD  - Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 1 
      Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
FAU - Yuk, Seong-Su
AU  - Yuk SS
AD  - Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 1 
      Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
FAU - Kwon, Jung-Hoon
AU  - Kwon JH
AD  - Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 1 
      Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
FAU - Hong, Woo-Tack
AU  - Hong WT
AD  - Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 1 
      Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
FAU - Gwon, Gyeong-Bin
AU  - Gwon GB
AD  - Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 1 
      Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
FAU - Lee, Jung-Ah
AU  - Lee JA
AD  - Division of Vaccine Research, Korea National Institute of Health, Korea Centers 
      for Disease Control and Prevention, Osong-eup, CheongJu 363-951, 
      Chungcheongbuk-do, Republic of Korea.
FAU - Lee, Joong-Bok
AU  - Lee JB
AD  - Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 1 
      Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
FAU - Lee, Sang-Won
AU  - Lee SW
AD  - Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 1 
      Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
FAU - Song, Chang-Seon
AU  - Song CS
AD  - Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 1 
      Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea. Electronic address: 
      songcs@konkuk.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20150726
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - *Adaptation, Biological
MH  - Animals
MH  - Chickens
MH  - Coronavirus Infections/prevention & control/*veterinary
MH  - Hot Temperature
MH  - Infectious bronchitis virus/*immunology/isolation & purification
MH  - Kidney/pathology
MH  - Poultry Diseases/immunology/*prevention & control
MH  - Serial Passage
MH  - Survival Analysis
MH  - Trachea/pathology
MH  - Vaccines, Attenuated/administration & dosage/adverse effects/immunology/isolation 
      & purification
MH  - Viral Vaccines/administration & dosage/adverse effects/*immunology/isolation & 
      purification
PMC - PMC7130781
OTO - NOTNLM
OT  - Attenuation
OT  - Cross-protective efficacy
OT  - Heat adaptation
OT  - Infectious bronchitis virus
OT  - Safety
EDAT- 2015/07/29 06:00
MHDA- 2016/10/07 06:00
PMCR- 2015/07/26
CRDT- 2015/07/29 06:00
PHST- 2015/05/26 00:00 [received]
PHST- 2015/07/13 00:00 [revised]
PHST- 2015/07/15 00:00 [accepted]
PHST- 2015/07/29 06:00 [entrez]
PHST- 2015/07/29 06:00 [pubmed]
PHST- 2016/10/07 06:00 [medline]
PHST- 2015/07/26 00:00 [pmc-release]
AID - S0264-410X(15)01002-6 [pii]
AID - 10.1016/j.vaccine.2015.07.043 [doi]
PST - ppublish
SO  - Vaccine. 2015 Dec 16;33(51):7370-7374. doi: 10.1016/j.vaccine.2015.07.043. Epub 
      2015 Jul 26.