PMID- 26220904
OWN - NLM
STAT- MEDLINE
DCOM- 20160920
LR  - 20191210
IS  - 1872-9754 (Electronic)
IS  - 0197-0186 (Linking)
VI  - 90
DP  - 2015 Nov
TI  - Carvacrol attenuates traumatic neuronal injury through store-operated Ca(2+) 
      entry-independent regulation of intracellular Ca(2+) homeostasis.
PG  - 107-13
LID - S0197-0186(15)30022-X [pii]
LID - 10.1016/j.neuint.2015.07.020 [doi]
AB  - Searching for effective pharmacological agents for traumatic brain injury (TBI) 
      treatment has largely been unsuccessful. The transient receptor potential 
      melastatin 7 (TRPM7), a TRP channel that is essential for embryonic development, 
      has been shown to mediate ischemic neuronal injury in vivo and in vitro, but 
      global deletion of TRPM7 in mice is lethal. Here, carvacrol was used to 
      investigate the protective effect of TRPM7 inhibition in an in vitro traumatic 
      neuronal injury model. Carvacrol (0.5 and 1 mM) reduced lactate dehydrogenase 
      (LDH) release, apoptosis and caspase-3 activation after traumatic injury in 
      cortical neurons. These neuroprotective effects were accompanied by alleviated 
      cytoplasmic calcium levels as measured by calcium imaging. In contrast, the 
      thapsigargin (TG) induced store-operated calcium entry (SOCE) and the expression 
      of SOCE related proteins in neurons were not altered by carvacrol treatment. The 
      involvement of TRPM7 sensitive calcium influx in our in vitro model was confirmed 
      by the results that bradykinin induced calcium influx was prevented by carvacrol 
      in neurons. Furthermore, carvacrol significantly inhibited the induction of 
      neuronal nitric oxide synthase (nNOS) after traumatic injury, and treatment with 
      carvacrol and the nNOS inhibitor NLPA together had no extra effect on calcium 
      concentration and neuronal injury. Thus, inhibition of TRPM7 function by 
      carvacrol protects against traumatic neuronal injury, and might be a potential 
      drug development strategy for the treatment of TBI.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Li, Wen-Tao
AU  - Li WT
AD  - Department of Neurosurgery, the First Affiliated Hospital, Medical School of 
      Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
FAU - Zhang, Su-Yuan
AU  - Zhang SY
AD  - Library of the First Affiliated Hospital, Medical School of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Zhou, Yue-Fei
AU  - Zhou YF
AD  - Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, 
      Xi'an, Shaanxi 710032, China.
FAU - Zhang, Bin-Fei
AU  - Zhang BF
AD  - Department of Neurosurgery, the First Affiliated Hospital, Medical School of 
      Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
FAU - Liang, Zhen-Qiang
AU  - Liang ZQ
AD  - Department of Neurosurgery, the Dingxi First People's Hospital, Dingxi, Gansu 
      743000, China.
FAU - Liu, Yong-Hong
AU  - Liu YH
AD  - Department of Neurosurgery, the Northwest Civil Aviation Hospital, Xi'an, Shaanxi 
      710061, China.
FAU - Wei, Yan
AU  - Wei Y
AD  - Department of Neurosurgery, the First Affiliated Hospital, Medical School of 
      Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
FAU - Li, Chuan-Kun
AU  - Li CK
AD  - Department of Neurosurgery, the First Affiliated Hospital, Medical School of 
      Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
FAU - Meng, Xi-Jun
AU  - Meng XJ
AD  - Department of Neurosurgery, the First Affiliated Hospital, Medical School of 
      Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
FAU - Xia, Ming
AU  - Xia M
AD  - Department of Neurosurgery, the First Affiliated Hospital, Medical School of 
      Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
FAU - Dan, Yong
AU  - Dan Y
AD  - Department of Neurosurgery, the First Affiliated Hospital, Medical School of 
      Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
FAU - Song, Jin-Ning
AU  - Song JN
AD  - Department of Neurosurgery, the First Affiliated Hospital, Medical School of 
      Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China. Electronic address: 
      songjinning_xjtu@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150726
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Calcium Channels)
RN  - 0 (Cymenes)
RN  - 0 (Monoterpenes)
RN  - 0 (Neuroprotective Agents)
RN  - 9B1J4V995Q (carvacrol)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Brain Injuries/*drug therapy/metabolism
MH  - Calcium/*metabolism
MH  - Calcium Channels/*drug effects/metabolism
MH  - Calcium Signaling/*drug effects
MH  - Cells, Cultured
MH  - Cymenes
MH  - Disease Models, Animal
MH  - Homeostasis/*drug effects
MH  - Intracellular Space/drug effects/metabolism
MH  - Monoterpenes/*pharmacology
MH  - Neurons/*drug effects/metabolism
MH  - Neuroprotective Agents/pharmacology
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Calcium homeostasis
OT  - TRPM7
OT  - Traumatic brain injury
OT  - nNOS
EDAT- 2015/07/30 06:00
MHDA- 2016/09/22 06:00
CRDT- 2015/07/30 06:00
PHST- 2015/04/30 00:00 [received]
PHST- 2015/07/18 00:00 [revised]
PHST- 2015/07/24 00:00 [accepted]
PHST- 2015/07/30 06:00 [entrez]
PHST- 2015/07/30 06:00 [pubmed]
PHST- 2016/09/22 06:00 [medline]
AID - S0197-0186(15)30022-X [pii]
AID - 10.1016/j.neuint.2015.07.020 [doi]
PST - ppublish
SO  - Neurochem Int. 2015 Nov;90:107-13. doi: 10.1016/j.neuint.2015.07.020. Epub 2015 
      Jul 26.