PMID- 26221902
OWN - NLM
STAT- MEDLINE
DCOM- 20160314
LR  - 20181202
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 19
IP  - 14
DP  - 2015
TI  - miR-206 inhibits human laryngeal squamous cell carcinoma cell growth by 
      regulation of cyclinD2.
PG  - 2697-702
LID - 9244 [pii]
AB  - OBJECTIVE: Accumulating evidence has shown that microRNAs (miRNAs) are aberrantly 
      expressd in many malignancies and crucial to tumorigenesis. Herein, we identified 
      the role and mechanism of miR-206 in laryngeal squamous cell carcinoma (LSCC) 
      growth. PATIENTS AND METHODS: Quantitative real-time PCR was performed to detect 
      the relative expression level of miR-206 in LSCC tissues. Crystal violet and flow 
      cytometry were conducted to explore the effects of miR-206 on the proliferation 
      and cell cycle of human LSCC cell line, respectively. The impact of miR-206 
      overexpression on putative target cyclinD2 were subsequently verified via Western 
      blot. Tumor growth assay was performed to testify the effect of miR-206 on the 
      tumor growth in vivo. RESULTS: MiR-206 expression was frequently (p < 0.05) 
      down-regulated in LSCC specimens. Overexpression of miR-206 in Hep-2 cell 
      inhibited the proliferation by blocking the G1/S transition as well as suppressed 
      the growth of xenograft tumors in mice, implying that miR-206 functions as a 
      tumour suppressor in the progression of LSCC. Overexpression of miR-206 
      significantly decreased (p < 0.05) the protein level of cyclinD2, which has 
      previously been identified as a direct targets of miR-206. CONCLUSIONS: 
      Altogether, our results identify a crucial tumour suppressive role of miR-206 in 
      LSCC growth, at least partly via up-regulation of cyclinD2 protein levels, and 
      suggest that miR-206 might be a candidate prognostic predictor or an anticancer 
      therapeutic target for LSCC patients.
FAU - Yu, W F
AU  - Yu WF
AD  - Department of Otolaryngology, the First Affiliated Hospital of Xinxiang Medical 
      College, Xinxiang, Henan, P. R. China. yu_wenfa@yahoo.com.
FAU - Wang, H M
AU  - Wang HM
FAU - Lu, B C
AU  - Lu BC
FAU - Zhang, G Z
AU  - Zhang GZ
FAU - Ma, H M
AU  - Ma HM
FAU - Wu, Z Y
AU  - Wu ZY
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (CCND2 protein, human)
RN  - 0 (Cyclin D2)
RN  - 0 (MIRN206 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Cell Cycle/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*physiology
MH  - Cyclin D2/*physiology
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Hep G2 Cells
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*metabolism/pathology/*prevention & control
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/*biosynthesis/genetics
EDAT- 2015/07/30 06:00
MHDA- 2016/03/15 06:00
CRDT- 2015/07/30 06:00
PHST- 2015/07/30 06:00 [entrez]
PHST- 2015/07/30 06:00 [pubmed]
PHST- 2016/03/15 06:00 [medline]
AID - 9244 [pii]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2015;19(14):2697-702.