PMID- 26222022
OWN - NLM
STAT- MEDLINE
DCOM- 20160222
LR  - 20181113
IS  - 1678-8060 (Electronic)
IS  - 0074-0276 (Print)
IS  - 0074-0276 (Linking)
VI  - 110
IP  - 5
DP  - 2015 Aug
TI  - Activation and cytokine profile of monocyte derived dendritic cells in leprosy: 
      in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of 
      IL-12p70 in lepromatous leprosy.
PG  - 655-61
LID - S0074-02762015005040230 [pii]
LID - 10.1590/0074-02760140230 [doi]
AB  - Dendritic cells (DCs) play a pivotal role in the connection of innate and 
      adaptive immunity of hosts to mycobacterial infection. Studies on the interaction 
      of monocyte-derived DCs (MO-DCs) using Mycobacterium leprae in leprosy patients 
      are rare. The present study demonstrated that the differentiation of MOs to DCs 
      was similar in all forms of leprosy compared to normal healthy individuals. In 
      vitro stimulation of immature MO-DCs with sonicated M. leprae induced variable 
      degrees of DC maturation as determined by the increased expression of HLA-DR, 
      CD40, CD80 and CD86, but not CD83, in all studied groups. The production of 
      different cytokines by the MO-DCs appeared similar in all of the studied groups 
      under similar conditions. However, the production of interleukin (IL)-12p70 by 
      MO-DCs from lepromatous (LL) leprosy patients after in vitro stimulation with M. 
      leprae was lower than tuberculoid leprosy patients and healthy individuals, even 
      after CD40 ligation with CD40 ligand-transfected cells. The present cumulative 
      findings suggest that the MO-DCs of LL patients are generally a weak producer of 
      IL-12p70 despite the moderate activating properties ofM. leprae. These results 
      may explain the poor M. leprae-specific cell-mediated immunity in the LL type of 
      leprosy.
FAU - Braga, Andre Flores
AU  - Braga AF
AD  - Faculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, 
      BR.
FAU - Moretto, Daniela Ferraz
AU  - Moretto DF
AD  - Universidade de Santo Amaro, Sao Paulo, SP, BR.
FAU - Gigliotti, Patricia
AU  - Gigliotti P
AD  - Faculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP, 
      BR.
FAU - Peruchi, Mariela
AU  - Peruchi M
AD  - Faculdade Anhanguera de Bauru, Bauru, SP, BR.
FAU - Vilani-Moreno, Fatima Regina
AU  - Vilani-Moreno FR
AD  - Instituto Lauro de Souza Lima, Bauru, SP, BR.
FAU - Campanelli, Ana Paula
AU  - Campanelli AP
AD  - Departamento de Microbiologia e Imunologia, Faculdade de Odontologia de Bauru, 
      Universidade de Sao Paulo, Bauru, SP, BR.
FAU - Latini, Ana Carla Pereira
AU  - Latini AC
AD  - Instituto Lauro de Souza Lima, Bauru, SP, BR.
FAU - Iyer, Anand
AU  - Iyer A
AD  - Department of Pathology, Academic Medical Center, University of Amsterdam, 
      Amsterdam, North Holland, NL.
FAU - Das, Pranab Kumar
AU  - Das PK
AD  - Instituto Lauro de Souza Lima, Bauru, SP, BR.
FAU - Souza, Vania Nieto Brito de
AU  - Souza VN
AD  - Instituto Lauro de Souza Lima, Bauru, SP, BR.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150724
PL  - Brazil
TA  - Mem Inst Oswaldo Cruz
JT  - Memorias do Instituto Oswaldo Cruz
JID - 7502619
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Cytokines)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Antigens, Bacterial/immunology
MH  - Case-Control Studies
MH  - Cytokines/*biosynthesis
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-12/immunology
MH  - Leprosy, Lepromatous/*immunology
MH  - Male
MH  - Monocytes/*immunology
MH  - Mycobacterium leprae/*immunology
MH  - Retrospective Studies
PMC - PMC4569830
EDAT- 2015/07/30 06:00
MHDA- 2016/02/24 06:00
PMCR- 2015/08/01
CRDT- 2015/07/30 06:00
PHST- 2014/06/26 00:00 [received]
PHST- 2015/07/01 00:00 [accepted]
PHST- 2015/07/30 06:00 [entrez]
PHST- 2015/07/30 06:00 [pubmed]
PHST- 2016/02/24 06:00 [medline]
PHST- 2015/08/01 00:00 [pmc-release]
AID - S0074-02762015005040230 [pii]
AID - 10.1590/0074-02760140230 [doi]
PST - ppublish
SO  - Mem Inst Oswaldo Cruz. 2015 Aug;110(5):655-61. doi: 10.1590/0074-02760140230. 
      Epub 2015 Jul 24.