PMID- 26222206
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20220409
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 40
IP  - 1
DP  - 2016 Jan
TI  - Enhancement of angiogenic effects by hypoxia-preconditioned human umbilical 
      cord-derived mesenchymal stem cells in a mouse model of hindlimb ischemia.
PG  - 27-35
LID - 10.1002/cbin.10519 [doi]
AB  - It has been studied that mesenchymal stem cells (MSCs) have the capability to 
      promote angiogenesis. Furthermore, there is strong evidence that hypoxic 
      conditions can enhance angiogenesis and immune modulation mediated by MSCs, a 
      notion that has been applied in many fields of clinical application. In the 
      present study, we compared the efficacy of hypoxia preconditioned human umbilical 
      cord-derived mesenchymal stem cells (hUC-MSCs) and normoxia conditioned hUC-MSCs 
      for the treatment of ischemic injury in hindlimbs of an immunodeficient mouse 
      model. Expression of negative markers for MSC such as CD31, CD34, and CD45 or 
      positive markers such as CD44, CD73, CD90, and CD105 was not significantly 
      changed in hypoxia preconditioned hUC-MSCs compared with hUC-MSCs cultured in 
      normoxic condition. Expression of angiogenesis-related genes such as COX-2, VEGF, 
      Tie-2, and TGF-beta1 was increased compared with hUC-MSCs cultured in normoxic 
      conditions. In the in vivo model, CD31 expression as a marker of angiogenesis was 
      significantly increased in the ischemic limbs at 1 month after injection with 
      hypoxic hUC-MSCs. Angiogenesis-related genes such as Ang-1, COX-1, PIGF, and 
      MCP-1 were significantly upregulated in the muscle of ischemic hindlimbs treated 
      with hypoxic hUC-MSCs than normoxic hUC-MSCs. Expression of proinflammatory genes 
      such as IL-1, and IL-20 was reduced, whereas TGF-beta1, which has an 
      anti-inflammatory effect, was strongly increased. In conclusion, hypoxic culture 
      conditions could induce expression of angiogenesis related genes in hUC-MSCs, and 
      hypoxia preconditioned hUC-MSCs showed enhancing effects by inducing angiogenesis 
      and low inflammatory immune response compared with normoxic hUC-MSCs in the 
      ischemia injured hindlimb of immunodeficient mice.
CI  - (c) 2015 International Federation for Cell Biology.
FAU - Han, Kyu-Hyun
AU  - Han KH
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Kim, Ae-Kyeong
AU  - Kim AK
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Kim, Min-Hee
AU  - Kim MH
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Kim, Do-Hyung
AU  - Kim DH
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Go, Ha-Nl
AU  - Go HN
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Kim, Dong-Ik
AU  - Kim DI
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150817
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Interleukins)
RN  - 0 (Transforming Growth Factor beta1)
RN  - U91R7IMG8U (interleukin 20)
SB  - IM
MH  - Angiogenesis Inducing Agents/*metabolism
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Cell Hypoxia/physiology
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Hindlimb/*blood supply
MH  - Humans
MH  - Interleukins/metabolism
MH  - Ischemia/*therapy
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Transforming Growth Factor beta1/metabolism
MH  - Umbilical Cord/cytology
OTO - NOTNLM
OT  - angiogenesis
OT  - hypoxia
OT  - immunodeficient
OT  - inflammatory
OT  - mesenchymal stem cell (MSC)
OT  - normoxia
EDAT- 2015/07/30 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/07/30 06:00
PHST- 2015/07/03 00:00 [received]
PHST- 2015/07/26 00:00 [accepted]
PHST- 2015/07/30 06:00 [entrez]
PHST- 2015/07/30 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1002/cbin.10519 [doi]
PST - ppublish
SO  - Cell Biol Int. 2016 Jan;40(1):27-35. doi: 10.1002/cbin.10519. Epub 2015 Aug 17.