PMID- 26223848
OWN - NLM
STAT- MEDLINE
DCOM- 20160304
LR  - 20240104
IS  - 1098-5557 (Electronic)
IS  - 1092-2172 (Print)
IS  - 1092-2172 (Linking)
VI  - 79
IP  - 3
DP  - 2015 Sep
TI  - An Overview of Genome Organization and How We Got There: from FISH to Hi-C.
PG  - 347-72
LID - 10.1128/MMBR.00006-15 [doi]
AB  - In humans, nearly two meters of genomic material must be folded to fit inside 
      each micrometer-scale cell nucleus while remaining accessible for gene 
      transcription, DNA replication, and DNA repair. This fact highlights the need for 
      mechanisms governing genome organization during any activity and to maintain the 
      physical organization of chromosomes at all times. Insight into the functions and 
      three-dimensional structures of genomes comes mostly from the application of 
      visual techniques such as fluorescence in situ hybridization (FISH) and molecular 
      approaches including chromosome conformation capture (3C) technologies. Recent 
      developments in both types of approaches now offer the possibility of exploring 
      the folded state of an entire genome and maybe even the identification of how 
      complex molecular machines govern its shape. In this review, we present key 
      methodologies used to study genome organization and discuss what they reveal 
      about chromosome conformation as it relates to transcription regulation across 
      genomic scales in mammals.
CI  - Copyright (c) 2015, American Society for Microbiology. All Rights Reserved.
FAU - Fraser, James
AU  - Fraser J
AD  - Department of Biochemistry, and Goodman Cancer Research Center, McGill 
      University, Montreal, Quebec, Canada.
FAU - Williamson, Iain
AU  - Williamson I
AD  - MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, 
      University of Edinburgh, Edinburgh, United Kingdom.
FAU - Bickmore, Wendy A
AU  - Bickmore WA
AD  - MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, 
      University of Edinburgh, Edinburgh, United Kingdom Wendy.Bickmore@igmm.ed.ac.uk 
      josee.dostie@mcgill.ca.
FAU - Dostie, Josee
AU  - Dostie J
AD  - Department of Biochemistry, and Goodman Cancer Research Center, McGill 
      University, Montreal, Quebec, Canada Wendy.Bickmore@igmm.ed.ac.uk 
      josee.dostie@mcgill.ca.
LA  - eng
GR  - MC_PC_U127527202/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Microbiol Mol Biol Rev
JT  - Microbiology and molecular biology reviews : MMBR
JID - 9706653
RN  - 0 (CCCTC-Binding Factor)
RN  - 0 (CTCF protein, human)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Animals
MH  - CCCTC-Binding Factor
MH  - Chromosome Mapping
MH  - Chromosomes, Human/genetics/ultrastructure
MH  - Gene Expression Regulation
MH  - *Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Repressor Proteins/physiology
PMC - PMC4517094
EDAT- 2015/08/01 06:00
MHDA- 2016/03/05 06:00
PMCR- 2016/07/29
CRDT- 2015/07/31 06:00
PHST- 2015/07/31 06:00 [entrez]
PHST- 2015/08/01 06:00 [pubmed]
PHST- 2016/03/05 06:00 [medline]
PHST- 2016/07/29 00:00 [pmc-release]
AID - 79/3/347 [pii]
AID - 00006-15 [pii]
AID - 10.1128/MMBR.00006-15 [doi]
PST - ppublish
SO  - Microbiol Mol Biol Rev. 2015 Sep;79(3):347-72. doi: 10.1128/MMBR.00006-15.