PMID- 26225139
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150730
LR  - 20240323
IS  - 1755-8166 (Print)
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 8
DP  - 2015
TI  - Comparative cytogenetics in the genus Hoplias (Characiformes, Erythrinidae) 
      highlights contrasting karyotype evolution among congeneric species.
PG  - 56
LID - 10.1186/s13039-015-0161-4 [doi]
LID - 56
AB  - BACKGROUND: The Erythrinidae fish family contains three genera, Hoplias, 
      Erythrinus and Hoplerythrinus widely distributed in Neotropical region. 
      Remarkably, species from this family are characterized by an extensive karyotype 
      diversity, with 2n ranging from 39 to 54 chromosomes and the occurrence of single 
      and/or multiple sex chromosome systems in some species. However, inside the 
      Hoplias genus, while H. malabaricus was subject of many studies, the cytogenetics 
      of other congeneric species remains poorly explored. In this study, we have 
      investigated chromosomal characteristics of four Hoplias species, namely H. 
      lacerdae, H. brasiliensis, H. intermedius and H. aimara. We used conventional 
      staining techniques (C-banding, Ag-impregnation and CMA3 -fluorescence) as well 
      as fluorescence in situ hybridization (FISH) with minor and major rDNA and 
      microsatellite DNAs as probes in order to analyze the karyotype evolution within 
      the genus. RESULTS: All species showed invariably 2n = 50 chromosomes and 
      practically identical karyotypes dominated only by meta- and submetacentric 
      chromosomes, the absence of heteromorphic sex chromosomes, similar pattern of 
      C-positive heterochromatin blocks and homologous Ag-NOR-bearing pairs. The 
      cytogenetic mapping of five repetitive DNA sequences revealed some particular 
      interspecific differences between them. However, the examined chromosomal 
      characteristics indicate that their speciation was not associated with major 
      changes in their karyotypes. CONCLUSION: Such conserved karyotypes contrasts with 
      the extensive karyotype diversity that has been observed in other Erythrinidae 
      species, particularly in the congeneric species H. malabaricus. Nevertheless, 
      what forces drive such particularly different modes of karyotype evolution among 
      closely related species? Different life styles, population structure and inner 
      chromosomal characteristics related to similar cases in other vertebrate groups 
      can also account for the contrasting modes of karyotype evolution in Hoplias 
      genus.
FAU - de Oliveira, Ezequiel Aguiar
AU  - de Oliveira EA
AD  - Universidade Federal de Sao Carlos, Departamento de Genetica e Evolucao, Sao 
      Carlos, SP Brazil ; SEDUC-MT, Cuiaba, MT Brazil.
FAU - Bertollo, Luiz Antonio Carlos
AU  - Bertollo LA
AD  - Universidade Federal de Sao Carlos, Departamento de Genetica e Evolucao, Sao 
      Carlos, SP Brazil.
FAU - Yano, Cassia Fernanda
AU  - Yano CF
AD  - Universidade Federal de Sao Carlos, Departamento de Genetica e Evolucao, Sao 
      Carlos, SP Brazil.
FAU - Liehr, Thomas
AU  - Liehr T
AD  - Jena University Hospital, Friedrich Schiller University, Institute of Human 
      Genetics, Kollegiengasse 10, D-07743 Jena, Germany.
FAU - Cioffi, Marcelo de Bello
AU  - Cioffi Mde B
AD  - Universidade Federal de Sao Carlos, Departamento de Genetica e Evolucao, Sao 
      Carlos, SP Brazil.
LA  - eng
PT  - Journal Article
DEP - 20150730
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC4518567
OTO - NOTNLM
OT  - Chromosome change and speciation
OT  - FISH
OT  - Fish cytogenetics
OT  - Repetitive DNA
OT  - Trahiras
EDAT- 2015/08/01 06:00
MHDA- 2015/08/01 06:01
PMCR- 2015/07/30
CRDT- 2015/07/31 06:00
PHST- 2015/05/28 00:00 [received]
PHST- 2015/07/14 00:00 [accepted]
PHST- 2015/07/31 06:00 [entrez]
PHST- 2015/08/01 06:00 [pubmed]
PHST- 2015/08/01 06:01 [medline]
PHST- 2015/07/30 00:00 [pmc-release]
AID - 161 [pii]
AID - 10.1186/s13039-015-0161-4 [doi]
PST - epublish
SO  - Mol Cytogenet. 2015 Jul 30;8:56. doi: 10.1186/s13039-015-0161-4. eCollection 
      2015.