PMID- 26228242
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1469-8730 (Electronic)
IS  - 0967-1994 (Linking)
DP  - 2015 Jul 31
TI  - Relationship between Sloan-Kettering virus expression and mammalian follicular 
      development.
PG  - 1-9
AB  - Sloan-Kettering virus gene, a product of a cellular proto-oncogene c-Ski is a 
      unique nuclear pro-oncoprotein and belongs to the Ski/Sno proto-oncogene family. 
      The aim of the present study was to locate Ski protein in rat ovaries in order to 
      find insights into the possible involvement of Ski in follicular development. 
      First, expression of c-Ski mRNA in the ovaries of adult female rats was confirmed 
      by RT-PCR. Then, ovaries obtained on the day of estrus were subjected to 
      immunohistochemical analysis for Ski and proliferating cell nuclear antigen 
      (PCNA) in combination with terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL). RT-PCR and in situ hybridization revealed that c-Ski 
      mRNA was expressed in the ovaries of the adult rat on the day of estrous and 
      localized mainly in the granulose cells. Ski was expressed in granulosa cells 
      that were positive for TUNEL, but negative for PCNA, regardless of the shape and 
      size of follicles. Expression of Ski in TUNEL-positive granulosa cells, but not 
      in PCNA-positive granulosa cells, was also verified in rats having atretic 
      follicles with double staining. These results indicate that Ski is profoundly 
      expressed in the granulosa cells of atretic follicles, but not in growing 
      follicles. Based on the present findings, Ski may play a role in the apoptosis of 
      granulosa cells during follicular atresia.
FAU - Kim, Hyun
AU  - Kim H
AD  - Department of Veterinary Physiology,Graduate School of Agricultural and Life 
      Science,The University of Tokyo,1-1-1 Yayoi,Bunkyo-ku,Tokyo 113-8657,Japan.
FAU - Cho, Young Moo
AU  - Cho YM
AD  - Animal Genetic Resources Research Center,National Institute of Animal 
      Science,RDA,Namwon 590-832,Republic of Korea.
FAU - Ko, Yeoung-Gyu
AU  - Ko YG
AD  - Animal Biotechnology Division,National Institute of Animal Science,RDA,Suwon 
      441-706,Republic of Korea.
FAU - Choe, Changyong
AU  - Choe C
AD  - Animal Genetic Resources Research Center,National Institute of Animal 
      Science,RDA,Namwon 590-832,Republic of Korea.
FAU - Seong, Hwan-Hoo
AU  - Seong HH
AD  - Animal Genetic Resources Research Center,National Institute of Animal 
      Science,RDA,Namwon 590-832,Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20150731
PL  - England
TA  - Zygote
JT  - Zygote (Cambridge, England)
JID - 9309124
OTO - NOTNLM
OT  - Apoptosis
OT  - Follicular atresia
OT  - Granulosa cell
OT  - Ovary
OT  - Ski
EDAT- 2015/08/01 06:00
MHDA- 2015/08/01 06:00
CRDT- 2015/08/01 06:00
PHST- 2015/08/01 06:00 [entrez]
PHST- 2015/08/01 06:00 [pubmed]
PHST- 2015/08/01 06:00 [medline]
AID - S0967199415000362 [pii]
AID - 10.1017/S0967199415000362 [doi]
PST - aheadofprint
SO  - Zygote. 2015 Jul 31:1-9. doi: 10.1017/S0967199415000362.