PMID- 26231224
OWN - NLM
STAT- MEDLINE
DCOM- 20160309
LR  - 20181113
IS  - 2008-2231 (Electronic)
IS  - 1560-8115 (Print)
IS  - 1560-8115 (Linking)
VI  - 23
IP  - 1
DP  - 2015 Aug 1
TI  - Generation of stable ARE- driven reporter system for monitoring oxidative stress.
PG  - 38
LID - 10.1186/s40199-015-0122-9 [doi]
LID - 38
AB  - BACKGROUND: NF-E2-related factor2 (Nrf2)-antioxidant response element (ARE) 
      signaling pathway is the major defensive mechanism against oxidative stress and 
      is up regulated by specific antioxidants and oxidants to comprise the 
      chemoptotective response. Detection of ARE-activating compounds helps to develop 
      new drugs and identify/quantify the tension range of the oxidants. Important 
      reasons promoting this work are high throughput, rapid and inexpensive 
      experiments relative to the in vitro studies for ARE-Nrf2 pathway monitoring of 
      chemicals and environmental samples. METHODS: In this study hepatoma Huh7 
      reporter cell line was generated which contains a luciferase gene under the 
      control of an ARE. This is the first example of ARE construct containing one copy 
      of extended consensus response element. The cells were treated with hydroquinone 
      (HQ) and p-benzoquinone (BQ) (oxidative stress inducers) and the antioxidant, 
      curcumin. RESULTS: The luciferase activity was induced in a 
      concentration-dependent manner in a concentration range of 1-2 muM for BQ and HQ. 
      Curcumin was also validated as an ARE inducer in concentration above 10 muM. In 
      addition, this reporter cell line provides a rapid detection as early as 4 h to 
      respond to the ARE inducers. CONCLUSION: It is a powerful tool for the sensitive 
      and selective screening of chemicals, drugs and environmental samples for their 
      antioxidant and oxidant activities.
FAU - Motahari, Paria
AU  - Motahari P
AD  - Faculty of Biological Sciences, Department of Molecular Genetics, School of 
      Biological Sciences, Tarbiat Modares University, Jalal Ale Ahmad Highway, PO Box 
      14115-111, Tehran, Iran.
FAU - Sadeghizadeh, Majid
AU  - Sadeghizadeh M
AD  - Faculty of Biological Sciences, Department of Molecular Genetics, School of 
      Biological Sciences, Tarbiat Modares University, Jalal Ale Ahmad Highway, PO Box 
      14115-111, Tehran, Iran. sadeghma@modares.ac.ir.
FAU - Behmanesh, Mehrdad
AU  - Behmanesh M
AD  - Faculty of Biological Sciences, Department of Molecular Genetics, School of 
      Biological Sciences, Tarbiat Modares University, Jalal Ale Ahmad Highway, PO Box 
      14115-111, Tehran, Iran.
FAU - Sabri, Shaghayegh
AU  - Sabri S
AD  - Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Zolghadr, Fatemeh
AU  - Zolghadr F
AD  - Faculty of Biological Sciences, Department of Molecular Genetics, School of 
      Biological Sciences, Tarbiat Modares University, Jalal Ale Ahmad Highway, PO Box 
      14115-111, Tehran, Iran. zolghadr@modares.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20150801
PL  - Switzerland
TA  - Daru
JT  - Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences
JID - 101125969
RN  - 0 (Antioxidants)
RN  - 0 (Benzoquinones)
RN  - 0 (Hydroquinones)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Oxidants)
RN  - 3T006GV98U (quinone)
RN  - EC 1.13.12.7 (Luciferases, Firefly)
RN  - IT942ZTH98 (Curcumin)
RN  - XV74C1N1AE (hydroquinone)
SB  - IM
MH  - Antioxidant Response Elements/*genetics
MH  - Antioxidants/pharmacology
MH  - Benzoquinones/pharmacology
MH  - Cell Line, Tumor
MH  - Curcumin/pharmacology
MH  - *Genes, Reporter
MH  - Humans
MH  - Hydroquinones/pharmacology
MH  - Luciferases, Firefly/genetics
MH  - NF-E2-Related Factor 2/*genetics
MH  - Oxidants/pharmacology
MH  - *Oxidative Stress/drug effects
PMC - PMC4521422
EDAT- 2015/08/02 06:00
MHDA- 2016/03/10 06:00
PMCR- 2015/08/01
CRDT- 2015/08/02 06:00
PHST- 2015/04/29 00:00 [received]
PHST- 2015/07/24 00:00 [accepted]
PHST- 2015/08/02 06:00 [entrez]
PHST- 2015/08/02 06:00 [pubmed]
PHST- 2016/03/10 06:00 [medline]
PHST- 2015/08/01 00:00 [pmc-release]
AID - 10.1186/s40199-015-0122-9 [pii]
AID - 122 [pii]
AID - 10.1186/s40199-015-0122-9 [doi]
PST - epublish
SO  - Daru. 2015 Aug 1;23(1):38. doi: 10.1186/s40199-015-0122-9.