PMID- 26236109
OWN - NLM
STAT- MEDLINE
DCOM- 20160502
LR  - 20181113
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2015
DP  - 2015
TI  - Pegylated Interferon-alpha Modulates Liver Concentrations of Activin-A and Its 
      Related Proteins in Normal Wistar Rat.
PG  - 414207
LID - 10.1155/2015/414207 [doi]
LID - 414207
AB  - AIMS: To measure the expression of activin betaA-subunit, activin IIA and IIB 
      receptors, Smad4, Smad7, and follistatin in the liver and the liver and serum 
      concentrations of mature activin-A and follistatin in normal rat following 
      treatment with pegylated interferon-alpha (Peg-INF-alpha) and ribavirin (RBV). MATERIALS 
      AND METHODS: 40 male Wistar rats were divided equally into 4 groups: "control," 
      "Peg-only" receiving 4 injections of Peg-INF-alpha (6 microg/rat/week), "RBV-only" 
      receiving ribavirin (4 mg/rat/day) orally, and "Peg & RBV" group receiving both 
      drugs. The expression of candidate molecules in liver was measured by 
      immunohistochemistry and quantitative PCR. The concentrations of mature proteins 
      in serum and liver homogenate samples were measured using ELISA. RESULTS: 
      Peg-INF-alpha  +/- RBV altered the expression of all candidate molecules in the liver 
      at the gene and protein levels (P < 0.05) and decreased activin-A and increased 
      follistatin in serum and liver homogenates compared with the other groups (P < 
      0.05). There were also significant correlations between serum and liver activin-A 
      and follistatin. CONCLUSION: Peg-INF-alpha modulates the hepatic production of 
      activin-A and follistatin, which can be detected in serum. Further studies are 
      needed to explore the role of Peg-INF-alpha on the production of activins and 
      follistatin by the liver and immune cells.
FAU - Refaat, Bassem
AU  - Refaat B
AD  - Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura 
      University, P.O. Box 7607, Al Abdeyah, Makkah, Saudi Arabia.
FAU - El-Shemi, Adel Galal
AU  - El-Shemi AG
AD  - Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura 
      University, P.O. Box 7607, Al Abdeyah, Makkah, Saudi Arabia ; Department of 
      Pharmacology, Faculty of Medicine, Assiut University, Assiut 6515, Egypt.
FAU - Ashshi, Ahmed Mohamed
AU  - Ashshi AM
AD  - Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura 
      University, P.O. Box 7607, Al Abdeyah, Makkah, Saudi Arabia.
FAU - Mahamid, Elaf Wael
AU  - Mahamid EW
AD  - Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura 
      University, P.O. Box 7607, Al Abdeyah, Makkah, Saudi Arabia.
FAU - Al-Qadi, Noha Mohammed
AU  - Al-Qadi NM
AD  - Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura 
      University, P.O. Box 7607, Al Abdeyah, Makkah, Saudi Arabia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150706
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Follistatin)
RN  - 0 (Interferon-alpha)
RN  - 0 (activin A)
RN  - 104625-48-1 (Activins)
SB  - IM
MH  - Activins/*blood/*metabolism
MH  - Animals
MH  - Follistatin/blood/metabolism
MH  - Interferon-alpha/*pharmacology
MH  - Liver/*drug effects/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Wistar
PMC - PMC4506924
EDAT- 2015/08/04 06:00
MHDA- 2016/05/03 06:00
PMCR- 2015/07/06
CRDT- 2015/08/04 06:00
PHST- 2015/02/27 00:00 [received]
PHST- 2015/05/30 00:00 [revised]
PHST- 2015/06/03 00:00 [accepted]
PHST- 2015/08/04 06:00 [entrez]
PHST- 2015/08/04 06:00 [pubmed]
PHST- 2016/05/03 06:00 [medline]
PHST- 2015/07/06 00:00 [pmc-release]
AID - 10.1155/2015/414207 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2015;2015:414207. doi: 10.1155/2015/414207. Epub 2015 Jul 6.