PMID- 26238942
OWN - NLM
STAT- MEDLINE
DCOM- 20160622
LR  - 20190820
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 12
IP  - 4
DP  - 2015 Oct
TI  - MicroRNA‑20a promotes the proliferation and cell cycle of human osteosarcoma 
      cells by suppressing early growth response 2 expression.
PG  - 4989-94
LID - 10.3892/mmr.2015.4098 [doi]
AB  - MicroRNAs (miRNAs) are crucial in cancer development. However, the underlying 
      mechanisms of miRNAs in osteosarcoma (OS) remain largely uncharacterized. The 
      present study investigated the role of miR‑20a in OS cell proliferation. It was 
      determined that miR‑20a expression is markedly upregulated in OS tissues and 
      cells compared with the matched adjacent normal tissues and h‑FOB human 
      osteoblast cell lines. Ectopic expression of miR‑20a promoted the proliferation 
      and anchorage‑independent growth of OS cells, whereas inhibition of miR‑20a 
      reduced this effect. Bioinformatics analysis further revealed early growth 
      response 2 (EGR2), as a potential target of miR‑20a. Data from luciferase 
      reporter assays showed that miR‑20a directly binds to the 3'‑untranslated region 
      (3'‑UTR) of EGR2 mRNA and represses expression at the transcriptional and 
      translational levels. In functional assays, miR‑20a promoted OS cell 
      proliferation and the cell cycle, which could be suppressed by an inhibitor of 
      miR‑20a. In conclusion, the data provide compelling evidence that miR‑20a 
      functions as an onco‑miRNA, which is important in promoting cell proliferation in 
      OS, and its oncogenic effect is mediated primarily through direct suppression of 
      EGR2 expression.
FAU - Zhuo, Wenkun
AU  - Zhuo W
AD  - Department of Orthopedics and Traumatology, Institute of Orthopedics and 
      Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, 
      Shanxi 710000, P.R. China.
FAU - Ge, Weiming
AU  - Ge W
AD  - Department of Orthopedics and Traumatology, The PLA Fifth Thirty Four Hospital, 
      Luoyang, Henan 471000, P.R. China.
FAU - Meng, Guolin
AU  - Meng G
AD  - Department of Orthopedics and Traumatology, Institute of Orthopedics and 
      Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, 
      Shanxi 710000, P.R. China.
FAU - Jia, Shuaijun
AU  - Jia S
AD  - Department of Orthopedics, Shanxi Hospital of Chinese Armed Police Forces, Xi'an, 
      Shanxi 710000, P.R. China.
FAU - Zhou, Xiang
AU  - Zhou X
AD  - Department of Orthopedics and Traumatology, Institute of Orthopedics and 
      Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, 
      Shanxi 710000, P.R. China.
FAU - Liu, Jian
AU  - Liu J
AD  - Department of Orthopedics and Traumatology, Institute of Orthopedics and 
      Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, 
      Shanxi 710000, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150720
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (3' Untranslated Regions)
RN  - 0 (CCND1 protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (EGR2 protein, human)
RN  - 0 (Early Growth Response Protein 2)
RN  - 0 (MIRN20a microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Oligonucleotides)
RN  - 0 (RNA, Small Interfering)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Base Sequence
MH  - Binding Sites
MH  - Bone Neoplasms/*genetics/metabolism/pathology
MH  - Cell Cycle/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cyclin D1/genetics/metabolism
MH  - Cyclin-Dependent Kinase Inhibitor p21/genetics/metabolism
MH  - Early Growth Response Protein 2/antagonists & inhibitors/*genetics/metabolism
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genes, Reporter
MH  - Humans
MH  - Luciferases/genetics/metabolism
MH  - Male
MH  - MicroRNAs/antagonists & inhibitors/*genetics/metabolism
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Oligonucleotides/genetics/metabolism
MH  - Osteoblasts/*metabolism/pathology
MH  - Osteosarcoma/*genetics/metabolism/pathology
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Signal Transduction
PMC - PMC4581803
EDAT- 2015/08/05 06:00
MHDA- 2016/06/23 06:00
PMCR- 2015/07/20
CRDT- 2015/08/05 06:00
PHST- 2014/09/30 00:00 [received]
PHST- 2015/06/25 00:00 [accepted]
PHST- 2015/08/05 06:00 [entrez]
PHST- 2015/08/05 06:00 [pubmed]
PHST- 2016/06/23 06:00 [medline]
PHST- 2015/07/20 00:00 [pmc-release]
AID - mmr-12-04-4989 [pii]
AID - 10.3892/mmr.2015.4098 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Oct;12(4):4989-94. doi: 10.3892/mmr.2015.4098. Epub 2015 Jul 
      20.