PMID- 26239068
OWN - NLM
STAT- MEDLINE
DCOM- 20160815
LR  - 20171116
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 99
IP  - 23
DP  - 2015 Dec
TI  - Improved insecticidal activity of a recombinant baculovirus expressing spider 
      venom cyto-insectotoxin.
PG  - 10261-9
LID - 10.1007/s00253-015-6846-0 [doi]
AB  - Baculoviruses have a long history of safe use as specific, environmentally 
      friendly insecticides that provide alternatives to chemical pesticides for 
      controlling insect pests. However, their use has been limited by several factors, 
      particularly their slow pathogenicity. In this study, we constructed a 
      recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) and an Autographa 
      californica multiple nucleopolyhedrovirus (AcMNPV) that expressed an 
      insect-specific cyto-insectotoxin (Cit1a) from the venom of the central Asian 
      spider Lachesana tarabaevi. Cit1a is a comparatively long linear cytolytic 
      molecule that contains a predicted alpha-helix structure composed of two short 
      membrane-acting antimicrobial peptides (MAMPs) that are joined together in a 
      "head-to-tail" shape. Cit1a fused to polyhedrin gene (polh) (polh-cit1a) was 
      expressed in the nuclei as polyhedra in silkworm larvae, Bm5 and Sf9 cells. An 
      early death of Bm5 and Sf9 cells by recombinant BmNPV/Polh-Cit1a and 
      AcMNPV/Polh-Cit1a was observed compared with control viruses that lacked the 
      toxin gene. The infected cells showed a loss of cytoplasm, membrane integrity, 
      and structural changes, suggesting that recombinant baculovirus-infected cells 
      were killed by the necrosis caused by Cit1a. In addition, the BmNPV/Polh-Cit1a 
      showed a significant reduction in the median lethal time (LT50) against silkworm 
      larvae compared with those of control BmNPV that lacked the cit1a gene.
FAU - Ali, M P
AU  - Ali MP
AD  - Laboratory of Biotechnology, Integrated Bioscience Section, Graduate School of 
      Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 
      422-8529, Japan. panna_ali@yahoo.com.
FAU - Kato, Tatsuya
AU  - Kato T
AD  - Laboratory of Biotechnology, Integrated Bioscience Section, Graduate School of 
      Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 
      422-8529, Japan. kato.tatsuya@shizuoka.ac.jp.
AD  - Research Institute of Green Science and Technology, Shizuoka University, 836 
      Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. kato.tatsuya@shizuoka.ac.jp.
FAU - Park, Enoch Y
AU  - Park EY
AD  - Laboratory of Biotechnology, Integrated Bioscience Section, Graduate School of 
      Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 
      422-8529, Japan. park.enoch@shizuoka.ac.jp.
AD  - Research Institute of Green Science and Technology, Shizuoka University, 836 
      Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. park.enoch@shizuoka.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150804
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Cytotoxins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Spider Venoms)
SB  - IM
MH  - Animals
MH  - Baculoviridae/*genetics/growth & development
MH  - Bombyx/physiology/virology
MH  - Cell Death
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cytotoxins/*biosynthesis/genetics/*toxicity
MH  - *Genetic Vectors
MH  - Insecta
MH  - Recombinant Fusion Proteins/*biosynthesis/genetics/*toxicity
MH  - *Spider Venoms
MH  - Survival Analysis
MH  - Time Factors
OTO - NOTNLM
OT  - Bombyx mori
OT  - Cyto-insectotoxin
OT  - Cytotoxicity
OT  - Pathogenicity
OT  - Recombinant baculovirus
EDAT- 2015/08/05 06:00
MHDA- 2016/08/16 06:00
CRDT- 2015/08/05 06:00
PHST- 2015/06/22 00:00 [received]
PHST- 2015/07/11 00:00 [accepted]
PHST- 2015/08/05 06:00 [entrez]
PHST- 2015/08/05 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
AID - 10.1007/s00253-015-6846-0 [pii]
AID - 10.1007/s00253-015-6846-0 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2015 Dec;99(23):10261-9. doi: 
      10.1007/s00253-015-6846-0. Epub 2015 Aug 4.