PMID- 26239318
OWN - NLM
STAT- MEDLINE
DCOM- 20160818
LR  - 20151110
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 210
DP  - 2015 Dec 2
TI  - Expression of antigenic epitopes of porcine reproductive and respiratory syndrome 
      virus (PRRSV) in a modified live-attenuated porcine circovirus type 2 (PCV2) 
      vaccine virus (PCV1-2a) as a potential bivalent vaccine against both PCV2 and 
      PRRSV.
PG  - 154-64
LID - S0168-1702(15)30031-9 [pii]
LID - 10.1016/j.virusres.2015.07.027 [doi]
AB  - Co-infection of pigs in the field with porcine circovirus type 2 (PCV2) and 
      porcine reproductive and respiratory syndrome virus (PRRSV) is common and poses a 
      major concern in effective control of PCV2 and PRRSV. We previously demonstrated 
      that insertion of foreign epitope tags in the C-terminus of PCV2 ORF2 produced 
      infectious virions that elicited humoral immune responses against both PCV2 
      capsid and inserted epitope tags. In this study, we aimed to determine whether 
      the non-pathogenic chimeric virus PCV1-2a, which is the basis for the licensed 
      PCV2 vaccine Fostera PCV, can express PRRSV antigenic epitopes, thus generating 
      dual immunity as a potential bivalent vaccine against both PCV2 and PPRSV. Four 
      different linear B-cell antigenic epitopes of PRRSV were inserted into the 
      C-terminus of the capsid gene of the PCV1-2a vaccine virus. We showed that 
      insertion of 12 (PRRSV-GP2 epitope II, PRRSV-GP3 epitope I, and PRRSV-GP5 epitope 
      I), and 14 (PRRSV-GP5 epitope IV) amino acid residues did not impair the 
      replication of the resulting PCV1-2a-PRRSVEPI chimeric viruses in vitro. The four 
      chimeric PCV1-2a viruses expressing PRRSV B-cell linear epitopes were 
      successfully rescued and characterized. An immunogenicity study in pigs revealed 
      that two of the four chimeric viruses, PCV1-2a-PRRSVEPIGP3IG and 
      PCV1-2a-PRRSVEPIEPIGP5IV, elicited neutralizing antibodies against PRRSV VR2385 
      as well as PCV2 (strains PCV2a, PCV2b, and mPCV2b). The results have important 
      implications for exploring the potential use of PCV1-2a vaccine virus as a live 
      virus vector to develop bivalent MLVs against both PCV2 and PRRSV.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Pineyro, Pablo E
AU  - Pineyro PE
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24060, USA; Department of Veterinary Diagnostic and Production 
      Animal Medicine, Iowa State University College of Veterinary Medicine, Ames, IA 
      5001, USA.
FAU - Kenney, Scott P
AU  - Kenney SP
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24060, USA.
FAU - Gimenez-Lirola, Luis G
AU  - Gimenez-Lirola LG
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University College of Veterinary Medicine, Ames, IA 5001, USA.
FAU - Heffron, C Lynn
AU  - Heffron CL
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24060, USA.
FAU - Matzinger, Shannon R
AU  - Matzinger SR
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24060, USA.
FAU - Opriessnig, Tanja
AU  - Opriessnig T
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University College of Veterinary Medicine, Ames, IA 5001, USA; The Roslin 
      Institute and The Royal (Dick) School of Veterinary Studies, University of 
      Edinburgh, Edinburgh, United Kingdom.
FAU - Meng, Xiang-Jin
AU  - Meng XJ
AD  - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24060, USA. Electronic address: xjmeng@vt.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150731
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Drug Carriers)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral/genetics/*immunology
MH  - Circovirus/genetics/*immunology
MH  - Drug Carriers
MH  - Epitopes, B-Lymphocyte/genetics/*immunology
MH  - Genetic Vectors
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Swine
MH  - Vaccines, Attenuated/administration & dosage/genetics/immunology
MH  - Vaccines, Synthetic/administration & dosage/genetics/immunology
MH  - Viral Vaccines/administration & dosage/genetics/*immunology
OTO - NOTNLM
OT  - Bivalent modified live-attenuated vaccine (MLV)
OT  - Chimeric PCV1-2 vaccine
OT  - Porcine circovirus type 2 (PCV2)
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
EDAT- 2015/08/05 06:00
MHDA- 2016/08/19 06:00
CRDT- 2015/08/05 06:00
PHST- 2015/06/28 00:00 [received]
PHST- 2015/07/28 00:00 [revised]
PHST- 2015/07/29 00:00 [accepted]
PHST- 2015/08/05 06:00 [entrez]
PHST- 2015/08/05 06:00 [pubmed]
PHST- 2016/08/19 06:00 [medline]
AID - S0168-1702(15)30031-9 [pii]
AID - 10.1016/j.virusres.2015.07.027 [doi]
PST - ppublish
SO  - Virus Res. 2015 Dec 2;210:154-64. doi: 10.1016/j.virusres.2015.07.027. Epub 2015 
      Jul 31.