PMID- 26242125
OWN - NLM
STAT- MEDLINE
DCOM- 20160426
LR  - 20181202
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 35
IP  - 6
DP  - 2015 Jun
TI  - [Effect of Guanmaitong Tablet on ERK and p38 Protein of TLR2 Pathway Expression 
      in Cerebral Ischemia/Reperfusion Rats: an Experimental Study].
PG  - 712-6
AB  - OBJECTIVE: To explore the inflammatory cascade mechanism through Toll like 
      receptor 2 (TLR2) pathway after cerebral ischemia/reperfusion, and to study 
      molecular mechanisms of Guanmaitong (GMT) Tablet for protecting brain damage. 
      METHODS: We used bolt-line method to block/release the middle cerebral artery, 
      causing cerebral ischemia/reperfusion (I/R) injury model. GMT Tablet was given by 
      gastrogavage. Rats were then divided into the high dose GMT group (1200 mg/kg), 
      the middle dose GMT group (600 mg/kg), the low dose GMT group (300 mg/kg), the 
      positive control group (Tanakan, 20 mg/kg). Their right brain tissues were fixed 
      in 10% neutral formalin. TLR2 expressions were detected by immunofluorescence 
      staining. The total protein was extracted from right brain tissues by 
      ultrasonica- tion. Expression levels of extracellular regulated protein kinases 
      (ERK), phospho-extracellular regulated protein kinases (p-ERK), p38-mitogen 
      activated protein kinases (p-ERK), phospho-p38-mitogen activated protein kinases 
      [p-p38-MAPKs(p-p38)] were assessed by Western blot. Abdominal aortic blood was 
      withdrawn. IL-6 and IL-1beta levels were detected by ELISA in brain tissues and 
      serum. RESULTS: Compared with the sham-oepration group, expression levels of 
      TLR2, ERK, p-ERK, p38, p-p38 protein were up-regulated (P < 0.05, P < 0.01), and 
      contents of IL-6 and IL-1beta in brain tissues and serum were increased in the model 
      group (P < 0.01). Expression levels of TLR2, ERK, p-ERK, p38, p-p38 were 
      down-regulated (P < 0.05, P < 0.01), and contents of IL-6 and IL-1beta were reduced 
      in brain tissues and serum in middle and high dose GMT groups (P < 0.05, P < 
      0.01). CONCLUSIONS: TLR2 pathway was involved in cerebral I/R injury. GMT 
      protected neurons by down-regulating protein expressions of TLR2, ERK, p-ERK, 
      p38, p-p38 and contents of IL-1beta and IL-6.
FAU - Zhang, Cui-xiang
AU  - Zhang CX
FAU - Liu, Jian-xun
AU  - Liu JX
FAU - Li, Dan
AU  - Li D
FAU - Li, Lei
AU  - Li L
FAU - Fu, Jian-hua
AU  - Fu JH
FAU - Hou, Jin-cai
AU  - Hou JC
FAU - Du, Xue-mei
AU  - Du XM
FAU - Zhang, Fa-chang
AU  - Zhang FC
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Tablets)
RN  - 0 (Toll-Like Receptor 2)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain Ischemia/*metabolism
MH  - Cerebral Infarction
MH  - Down-Regulation
MH  - Drugs, Chinese Herbal/*therapeutic use
MH  - Interleukin-1beta
MH  - Interleukin-6
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury
MH  - Tablets
MH  - Toll-Like Receptor 2/*metabolism
MH  - Up-Regulation
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
EDAT- 2015/08/06 06:00
MHDA- 2016/04/27 06:00
CRDT- 2015/08/06 06:00
PHST- 2015/08/06 06:00 [entrez]
PHST- 2015/08/06 06:00 [pubmed]
PHST- 2016/04/27 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2015 Jun;35(6):712-6.