PMID- 26244295
OWN - NLM
STAT- MEDLINE
DCOM- 20151027
LR  - 20221207
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 56
IP  - 9
DP  - 2015 Aug
TI  - Roles of Epithelial Cell-Derived Type 2-Initiating Cytokines in Experimental 
      Allergic Conjunctivitis.
PG  - 5194-202
LID - 10.1167/iovs.15-16563 [doi]
AB  - PURPOSE: To clarify the possible involvement of the type 2-initiating cytokines 
      interleukin (IL)-25, IL-33, and thymic stromal lymphopoietin (TSLP) in the 
      pathophysiology of allergic conjunctivitis, we evaluated ragweed (RW)-induced 
      experimental allergic conjunctivitis (EAC) models by using IL-25 knockout (KO), 
      IL-33 KO, and TSLP receptor (TSLPR) KO mice. METHODS: Interleukin-25 KO, IL-33 
      KO, TSLPR KO, and BALB/c wild-type mice were sensitized twice with RW in alum and 
      then challenged with RW in eye drops. Clinical scores and eosinophil infiltration 
      were evaluated. Expression levels of serum immunoglobulin E (IgE) and cytokines 
      in the conjunctival tissues were quantified and immunohistochemical analysis was 
      carried out. RESULTS: Significant reductions in clinical scores and numbers of 
      infiltrating eosinophils were observed in the RW-EAC model using IL-33 KO mice. 
      There were no significant differences in clinical scores and numbers of 
      infiltrating eosinophils among IL-25KO, TSLPR KO, and wild-type mice. Serum IgE 
      concentration was upregulated after RW challenges, and there were no differences 
      among the mouse genotypes. Expression levels of of il4, il5, il13, and ccl5 mRNA 
      were diminished in the conjunctivae of the RW-EAC model using IL-33 KO mice 
      compared to those in wild-type mice. Interleukin-33 expression was upregulated as 
      early as 1 hour after RW eye-drop challenge. The number of infiltrating basophils 
      in the conjunctivae of the RW-EAC model using IL-33 KO mice was diminished 
      compared to that in wild-type mice. CONCLUSIONS: Among the type 2-initiating 
      cytokines, IL-33 may play a major role in conjunctival inflammation in an RW-EAC 
      model.
FAU - Asada, Yosuke
AU  - Asada Y
AD  - Laboratory of Ocular Atopic Diseases Department of Ophthalmology, Juntendo 
      University School of Medicine, Tokyo, Japan 2Frontier Research Initiative, 
      Institute of Medical Science, University of Tokyo, Tokyo, Japan.
FAU - Nakae, Susumu
AU  - Nakae S
AD  - Frontier Research Initiative, Institute of Medical Science, University of Tokyo, 
      Tokyo, Japan.
FAU - Ishida, Waka
AU  - Ishida W
AD  - Department of Ophthalmology, Kochi University School of Medicine, Nangoku, Japan.
FAU - Hori, Kanji
AU  - Hori K
AD  - Laboratory of Ocular Atopic Diseases Department of Ophthalmology, Juntendo 
      University School of Medicine, Tokyo, Japan.
FAU - Sugita, Jobu
AU  - Sugita J
AD  - Laboratory of Ocular Atopic Diseases Department of Ophthalmology, Juntendo 
      University School of Medicine, Tokyo, Japan.
FAU - Sudo, Katsuko
AU  - Sudo K
AD  - Animal Research Center, Tokyo Medical University, Tokyo, Japan.
FAU - Fukuda, Ken
AU  - Fukuda K
AD  - Department of Ophthalmology, Kochi University School of Medicine, Nangoku, Japan.
FAU - Fukushima, Atsuki
AU  - Fukushima A
AD  - Department of Ophthalmology, Kochi University School of Medicine, Nangoku, Japan.
FAU - Suto, Hajime
AU  - Suto H
AD  - Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan 
      6Atopy Research Center, Juntendo University School of Medicine, Tokyo, Japan.
FAU - Murakami, Akira
AU  - Murakami A
AD  - Laboratory of Ocular Atopic Diseases Department of Ophthalmology, Juntendo 
      University School of Medicine, Tokyo, Japan.
FAU - Saito, Hirohisa
AU  - Saito H
AD  - Department of Allergy and Immunology, National Research Institute for Child 
      Health and Development, Tokyo, Japan.
FAU - Ebihara, Nobuyuki
AU  - Ebihara N
AD  - Laboratory of Ocular Atopic Diseases Department of Ophthalmology, Juntendo 
      University School of Medicine, Tokyo, Japan.
FAU - Matsuda, Akira
AU  - Matsuda A
AD  - Laboratory of Ocular Atopic Diseases Department of Ophthalmology, Juntendo 
      University School of Medicine, Tokyo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Cytokines)
RN  - 0 (Il33 protein, mouse)
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-33)
RN  - 0 (Interleukins)
RN  - 0 (Mydgf protein, mouse)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 63231-63-0 (RNA)
RN  - GT0IL38SP4 (Thymic Stromal Lymphopoietin)
RN  - 0 (TSLP protein, mouse)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Conjunctiva/immunology/*metabolism/pathology
MH  - Conjunctivitis, Allergic/*genetics/immunology/pathology
MH  - Cytokines/biosynthesis/*genetics
MH  - Disease Models, Animal
MH  - *Gene Expression Regulation
MH  - Immunoglobulin E/biosynthesis/genetics
MH  - Immunohistochemistry
MH  - Interleukin-17/biosynthesis/*genetics
MH  - Interleukin-33
MH  - Interleukins/biosynthesis/*genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Knockout
MH  - RNA/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Thymic Stromal Lymphopoietin
EDAT- 2015/08/06 06:00
MHDA- 2015/10/28 06:00
CRDT- 2015/08/06 06:00
PHST- 2015/08/06 06:00 [entrez]
PHST- 2015/08/06 06:00 [pubmed]
PHST- 2015/10/28 06:00 [medline]
AID - 2424447 [pii]
AID - 10.1167/iovs.15-16563 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2015 Aug;56(9):5194-202. doi: 10.1167/iovs.15-16563.