PMID- 26244581
OWN - NLM
STAT- MEDLINE
DCOM- 20160406
LR  - 20240331
IS  - 2224-9435 (Electronic)
IS  - 1019-9128 (Print)
IS  - 1019-9128 (Linking)
VI  - 86
IP  - 1
DP  - 2015 Jun 8
TI  - Molecular characterisation of Mycoplasma species isolated from the genital tract 
      of Dorper sheep in South Africa.
PG  - e1-e11
LID - 10.4102/jsava.v86i1.1199 [doi]
LID - 1199
AB  - Biochemical and molecular analysis were conducted on 34 strains of Mycoplasma 
      species isolated between 2003 and 2009 from the genital tract of clinically 
      healthy Dorper sheep and sheep with ulcerative vulvitis and balanitis. Earlier 
      publications identified the causative agent as Mycoplasma mycoides mycoides large 
      colony (MmmLC) and Arcanobacterium pyogenes. The aims of the study were to 
      characterise Mycoplasma species isolated from the genital tract of Dorper sheep 
      with polymerase chain reaction assay, cloning and gene sequencing. Basic Local 
      Alignment Search Tool (BLAST) results revealed six predominant Mycoplasma 
      species: Mycoplasma arginini, Mycoplasma bovigenitalium, Arcanobacterium 
      laidlawii, MmmLC, Mycoplasma sp. ovine/caprine serogroup II and M. canadense. 
      Sequencing of the 34 isolates were analysed using phylogenetic methods, and 18 
      (50%) were identified as M. arginini with 99% - 100% similarity to M. arginini 
      from England and Sweden. Six isolates showed 99% similarity to M. bovigenitalium 
      strains from Turkey and Germany. Two isolates had 99% similarity to an M. sp. 
      ovine/caprine sero group II from the United Kingdom. BLAST for two isolates 
      revealed 99% similarity to Acholeplasma laidlawii from India, another two were 
      99% similar to MmmLC strain from Sweden, two showed 98% similarity to Mycoplasma 
      sp. Usp 120 from Brazil, and two isolates have a 97% - 99% similarity to M. mm. 
      Jcv1 strain from the United States of America. Finally, one isolate showed 
      similarity of 99% to Mycoplasma canadense strain from Italy. The findings support 
      the hypothesis that ulcerative vulvitis and balanitis of Dorper sheep in South 
      Africa (SA) is a multifactorial disease with involvement of different Mycoplasma 
      species.
FAU - Kalshingi, Habu A
AU  - Kalshingi HA
AD  - National Veterinary Research Institute, Vom, Nigeria; Department of Veterinary 
      Tropical Diseases, University of Pretoria, South Africa. halisab67@yahoo.co.uk.
FAU - Bosman, Anna-Mari
AU  - Bosman AM
FAU - Gouws, Johan
AU  - Gouws J
FAU - van Vuuren, Moritz
AU  - van Vuuren M
LA  - eng
SI  - GENBANK/HQ661818
SI  - GENBANK/HQ661819
SI  - GENBANK/HQ661820
SI  - GENBANK/HQ661821
SI  - GENBANK/HQ661822
SI  - GENBANK/HQ661823
SI  - GENBANK/HQ661824
SI  - GENBANK/HQ661825
SI  - GENBANK/HQ661826
SI  - GENBANK/HQ661827
SI  - GENBANK/HQ661828
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150608
PL  - South Africa
TA  - J S Afr Vet Assoc
JT  - Journal of the South African Veterinary Association
JID - 7503122
SB  - IM
MH  - Animals
MH  - Balanitis/microbiology/*veterinary
MH  - Female
MH  - Male
MH  - Molecular Sequence Data
MH  - Mycoplasma/classification/*genetics
MH  - Mycoplasma Infections/microbiology/*veterinary
MH  - Phylogeny
MH  - Sequence Analysis, DNA/veterinary
MH  - Sheep
MH  - Sheep Diseases/*microbiology
MH  - South Africa
MH  - Vulvitis/microbiology/*veterinary
PMC - PMC6138128
COIS- The authors declare that they have no conflict of interest and financial or 
      personal relationships which may have inappropriately influenced them in writing 
      this article.
EDAT- 2015/08/06 06:00
MHDA- 2016/04/07 06:00
PMCR- 2015/06/08
CRDT- 2015/08/06 06:00
PHST- 2014/06/19 00:00 [received]
PHST- 2015/01/16 00:00 [accepted]
PHST- 2014/12/18 00:00 [revised]
PHST- 2015/08/06 06:00 [entrez]
PHST- 2015/08/06 06:00 [pubmed]
PHST- 2016/04/07 06:00 [medline]
PHST- 2015/06/08 00:00 [pmc-release]
AID - JSAVA-86-1199 [pii]
AID - 10.4102/jsava.v86i1.1199 [doi]
PST - epublish
SO  - J S Afr Vet Assoc. 2015 Jun 8;86(1):e1-e11. doi: 10.4102/jsava.v86i1.1199.