PMID- 26247176
OWN - NLM
STAT- MEDLINE
DCOM- 20160825
LR  - 20221207
IS  - 1873-2933 (Electronic)
IS  - 0009-9120 (Linking)
VI  - 48
IP  - 16-17
DP  - 2015 Nov
TI  - Identification of Hb Wayne and its effects on HbA1c measurement by 5 methods.
PG  - 1144-50
LID - S0009-9120(15)00380-X [pii]
LID - 10.1016/j.clinbiochem.2015.07.100 [doi]
AB  - BACKGROUND: The World Health Organization and the American and Canadian Diabetes 
      Associations approved HbA1c >6.5% as diagnostic for type 2 diabetes mellitus 
      (T2DM). Hb variants and/or their chemically modified species can interfere with 
      HbA1c measurements. We recently described a patient with Hb Wayne trait who was 
      misdiagnosed with T2DM based on falsely elevated HbA1c. Hb Wayne is a clinically 
      silent variant that exists as two isoforms: Hb Wayne I (Asn 139) and Hb Wayne II 
      (Asp 139). METHODS: Hemoglobinopathy investigation was performed by HPLC (Bio-Rad 
      VARIANT-II), alkaline and acid electrophoresis (Sebia Hydrasis2), capillary zone 
      electrophoresis (Sebia CAPILLARYS2) and DNA sequencing. HbA1c was measured by 
      five methods. RESULTS: Hb Wayne eluted as two small fractions with retention 
      times of 1.0 and 1.46min on the HPLC (Bio-Rad VARIANT-II). Alkaline gel and 
      capillary electrophoresis showed two small bands migrating faster than HbA. Hb 
      Wayne generated spuriously high results on the Bio-Rad VARIANT-II Turbo 2.0, no 
      results on the Tosoh G8, and did not interfere with either the Sebia CAPILLARYS2 
      or immunoassays from Roche (tinaquant) and Siemens (Bayer DCA2000+). Based on the 
      Hb Wayne HPLC profile of 3 patients, an algorithm was developed to facilitate its 
      detection, which identified 9 additional patients with Hb Wayne trait. 
      CONCLUSIONS: We characterize Hb Wayne by chromatographic and electrophoretic 
      techniques and show the effect of Hb Wayne on five common HbA1c methodologies. We 
      developed a quality assurance tool to assist in detecting Hb Wayne trait during 
      HbA1c analysis on the Bio-Rad VARIANT-II Turbo 2.0.
CI  - Copyright (c) 2015 The Canadian Society of Clinical Chemists. Published by Elsevier 
      Inc. All rights reserved.
FAU - Rodriguez-Capote, Karina
AU  - Rodriguez-Capote K
AD  - DynaLIFE(DX) Diagnostic Laboratory Services, Edmonton, AB, Canada; Dept 
      Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada. 
      Electronic address: karina.capote@dynalifedx.com.
FAU - Estey, Mathew P
AU  - Estey MP
AD  - DynaLIFE(DX) Diagnostic Laboratory Services, Edmonton, AB, Canada; Dept 
      Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
FAU - Barakauskas, Vilte E
AU  - Barakauskas VE
AD  - DynaLIFE(DX) Diagnostic Laboratory Services, Edmonton, AB, Canada; Dept 
      Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
FAU - Burton, Teralee
AU  - Burton T
AD  - Regina Qu'Appelle Health Region, Regina, SK, Canada.
FAU - Holmes, Deborah
AU  - Holmes D
AD  - DynaLIFE(DX) Diagnostic Laboratory Services, Edmonton, AB, Canada.
FAU - Krause, Richard
AU  - Krause R
AD  - Calgary Laboratory Services, Calgary, AB, Canada.
FAU - Higgins, Trefor N
AU  - Higgins TN
AD  - DynaLIFE(DX) Diagnostic Laboratory Services, Edmonton, AB, Canada; Dept 
      Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
LA  - eng
PT  - Journal Article
DEP - 20150804
PL  - United States
TA  - Clin Biochem
JT  - Clinical biochemistry
JID - 0133660
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (Hemoglobins, Abnormal)
RN  - 0 (hemoglobin A1c protein, human)
RN  - 0 (hemoglobin Wayne)
SB  - IM
MH  - Algorithms
MH  - Chromatography, High Pressure Liquid/methods
MH  - Diabetes Mellitus, Type 2/metabolism
MH  - Electrophoresis, Capillary/methods
MH  - Glycated Hemoglobin/*metabolism
MH  - Hemoglobinopathies/diagnosis/metabolism
MH  - Hemoglobins, Abnormal/*metabolism
MH  - Humans
MH  - Sequence Analysis, DNA/methods
OTO - NOTNLM
OT  - Diabetes
OT  - HPLC
OT  - HbA1c
OT  - Hemoglobinopathy
OT  - Interference
OT  - Quality assurance
EDAT- 2015/08/08 06:00
MHDA- 2016/08/26 06:00
CRDT- 2015/08/07 06:00
PHST- 2015/06/19 00:00 [received]
PHST- 2015/07/26 00:00 [revised]
PHST- 2015/07/31 00:00 [accepted]
PHST- 2015/08/07 06:00 [entrez]
PHST- 2015/08/08 06:00 [pubmed]
PHST- 2016/08/26 06:00 [medline]
AID - S0009-9120(15)00380-X [pii]
AID - 10.1016/j.clinbiochem.2015.07.100 [doi]
PST - ppublish
SO  - Clin Biochem. 2015 Nov;48(16-17):1144-50. doi: 10.1016/j.clinbiochem.2015.07.100. 
      Epub 2015 Aug 4.