PMID- 26247922
OWN - NLM
STAT- MEDLINE
DCOM- 20160523
LR  - 20150825
IS  - 1473-0189 (Electronic)
IS  - 1473-0189 (Linking)
VI  - 15
IP  - 18
DP  - 2015
TI  - A digital microfluidic device with integrated nanostructured microelectrodes for 
      electrochemical immunoassays.
PG  - 3776-84
LID - 10.1039/c5lc00660k [doi]
AB  - Nanostructured microelectrodes (NMEs) are three-dimensional electrodes that have 
      superb sensitivity for electroanalysis. Here we report the integration of NMEs 
      with the versatile fluid-handling system digital microfluidics (DMF), for 
      eventual application to distributed diagnostics outside of the laboratory. In the 
      new methods reported here, indium tin oxide DMF top plates were modified to 
      include Au NMEs as well as counter and pseudoreference electrodes. The new system 
      was observed to outperform planar sensing electrodes of the type that are 
      typically integrated with DMF. A rubella virus (RV) IgG immunoassay was developed 
      to evaluate the diagnostic potential for the new system, relying on magnetic 
      microparticles coated with RV particles and analysis by differential pulse 
      voltammetry. The limit of detection of the assay (0.07 IU mL(-1)) was >100x below 
      the World Health Organization defined cut-off for rubella immunity. The 
      sensitivity of the integrated device and its small size suggest future utility 
      for distributed diagnostics.
FAU - Rackus, Darius G
AU  - Rackus DG
AD  - Department of Chemistry, University of Toronto, 80 St. George St., Toronto, ON 
      M5S 3H6, Canada. shana.kelley@utoronto.ca aaron.wheeler@utoronto.ca.
FAU - Dryden, Michael D M
AU  - Dryden MD
FAU - Lamanna, Julian
AU  - Lamanna J
FAU - Zaragoza, Alexandre
AU  - Zaragoza A
FAU - Lam, Brian
AU  - Lam B
FAU - Kelley, Shana O
AU  - Kelley SO
FAU - Wheeler, Aaron R
AU  - Wheeler AR
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*chemistry
MH  - *Electrochemical Techniques/instrumentation/methods
MH  - Goats
MH  - Humans
MH  - Immunoassay/instrumentation/methods
MH  - Immunoglobulin G/*chemistry
MH  - *Lab-On-A-Chip Devices
MH  - Microelectrodes
MH  - Nanostructures/*chemistry
MH  - Rubella virus/*chemistry
EDAT- 2015/08/08 06:00
MHDA- 2016/05/24 06:00
CRDT- 2015/08/07 06:00
PHST- 2015/08/07 06:00 [entrez]
PHST- 2015/08/08 06:00 [pubmed]
PHST- 2016/05/24 06:00 [medline]
AID - 10.1039/c5lc00660k [doi]
PST - ppublish
SO  - Lab Chip. 2015;15(18):3776-84. doi: 10.1039/c5lc00660k.