PMID- 26250057
OWN - NLM
STAT- MEDLINE
DCOM- 20160718
LR  - 20181113
IS  - 1573-4994 (Electronic)
IS  - 1053-0509 (Linking)
VI  - 25
IP  - 5
DP  - 2015 Sep
TI  - Development of a Fluorescence-Linked Immunosorbent Assay for Baicalin.
PG  - 1371-6
LID - 10.1007/s10895-015-1627-9 [doi]
AB  - Previously, we developed an indirect competitive enzyme-linked immunosorbent 
      assay (icELISA) for baicalin (BAL) and used this assay to investigate the 
      pharmacokinetics of BAL in mice. In this study, an anti-BAL monoclonal antibody 
      (MAb) was purified by the caprylic acid method and then labelled with fluorescein 
      isothiocyanate (FITC). Subsequently, an indirect competitive fluorescence-linked 
      immunosorbent assay (icFLISA) was developed to detect baicalin (BAL) using 
      FITC-labelled anti-BAL MAbs. Characterization of the assay demonstrated an 
      effective BAL measurement range of 6.4 ng/mL to 500 mug/mL (R(2) = 0.997). The 
      relative standard deviations (RSDs) for both intra-assay and inter-assay 
      repeatability and precision were below 10 %. This icFLISA for BAL is simple, 
      rapid and sensitive, with a 390-fold larger linear range and a 2-fold lower limit 
      of detection (LOD) compared with the previously developed icELISA. We observed a 
      strong correlation between the results determined by the icFLISA and icELISA 
      methods. Overall, this study provides a useful method for detecting BAL in 
      medicines, enabling in vivo visualization research.
FAU - Shan, Wenchao
AU  - Shan W
AD  - School of Chinese Materia Medica, Beijing University of Chinese Medicine, 11 
      Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
FAU - Cheng, Jinjun
AU  - Cheng J
AD  - School of Chinese Materia Medica, Beijing University of Chinese Medicine, 11 
      Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
FAU - Qu, Baoping
AU  - Qu B
AD  - School of Basic Medical Sciences, Beijing University of Chinese Medicine, 11 
      Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
FAU - Sai, Jiayang
AU  - Sai J
AD  - The third affiliated hospital, Beijing University of Chinese Medicine, 11 
      Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
FAU - Kong, Hui
AU  - Kong H
AD  - School of Basic Medical Sciences, Beijing University of Chinese Medicine, 11 
      Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
FAU - Qu, Huihua
AU  - Qu H
AD  - Center of Scientific Experiment, Beijing University of Chinese Medicine, 11 
      Beisanhuandong Road, Chaoyang District, Beijing, 100029, China. 
      quhuihua@gmail.com.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - School of Basic Medical Sciences, Beijing University of Chinese Medicine, 11 
      Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
FAU - Wang, Qingguo
AU  - Wang Q
AD  - School of Basic Medical Sciences, Beijing University of Chinese Medicine, 11 
      Beisanhuandong Road, Chaoyang District, Beijing, 100029, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150807
PL  - Netherlands
TA  - J Fluoresc
JT  - Journal of fluorescence
JID - 9201341
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Flavonoids)
RN  - 347Q89U4M5 (baicalin)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Flavonoids/*analysis/immunology
MH  - *Immunosorbent Techniques
MH  - Mice
MH  - Spectrometry, Fluorescence
OTO - NOTNLM
OT  - Baicalin
OT  - Enzyme-linked immunosorbent assay
OT  - Fluorescence-linked immunosorbent assay
OT  - Fluorescently labelled monoclonal antibody
OT  - Traditional Chinese medicine
EDAT- 2015/08/08 06:00
MHDA- 2016/07/19 06:00
CRDT- 2015/08/08 06:00
PHST- 2015/05/11 00:00 [received]
PHST- 2015/07/26 00:00 [accepted]
PHST- 2015/08/08 06:00 [entrez]
PHST- 2015/08/08 06:00 [pubmed]
PHST- 2016/07/19 06:00 [medline]
AID - 10.1007/s10895-015-1627-9 [pii]
AID - 10.1007/s10895-015-1627-9 [doi]
PST - ppublish
SO  - J Fluoresc. 2015 Sep;25(5):1371-6. doi: 10.1007/s10895-015-1627-9. Epub 2015 Aug 
      7.