PMID- 26250158
OWN - NLM
STAT- MEDLINE
DCOM- 20160524
LR  - 20181113
IS  - 1674-8018 (Electronic)
IS  - 1674-800X (Print)
IS  - 1674-800X (Linking)
VI  - 6
IP  - 9
DP  - 2015 Sep
TI  - MicroRNA-638 inhibits cell proliferation by targeting phospholipase D1 in human 
      gastric carcinoma.
PG  - 680-688
LID - 10.1007/s13238-015-0187-8 [doi]
AB  - MicroRNAs (miRNAs) are a type of small non-coding RNAs that are often play 
      important roles in carcinogenesis, but the carcinogenic mechanism of miRNAs is 
      still unclear. This study will investigate the function and the mechanism of 
      miR-638 in carcinoma (GC). The expression of miR-638 in GC and the DNA copy 
      number of miR-638 were detected by real-time PCR. The effect of miR-638 on cell 
      proliferation was measured by counting kit-8 assay. Different assays, including 
      bioinformatics algorithms (TargetScan and miRanda), luciferase report assay and 
      Western blotting, were used to identify the target gene of miR-638 in GC. The 
      expression of miR-638 target gene in clinical CRC tissues was also validated by 
      immunohistochemical assay. From this research, we found that miR-638 was 
      downregulated in GC tissues compared with corresponding noncancerous tissues 
      (NCTs), and the DNA copy number of miR-638 was lower in GC than NCTs, which may 
      induce the corresponding downregulation of miR-638 in GC. Ectopic expression of 
      miR-638 inhibited GC cell growth in vitro. Subsequently, we identified that PLD1 
      is the target gene of miR-638 in GC, and silencing PLD1 expression phenocopied 
      the inhibitory effect of miR-638 on GC cell proliferation. Furthermore, we 
      observed that PLD1 was overexpressed in GC tissues, and high expression of PLD1 
      in GC predicted poor overall survival. In summary, we revealed that miR-638 
      functions as a tumor suppressor in GC through inhibiting PLD1.
FAU - Zhang, Jiwei
AU  - Zhang J
AD  - Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, Wuxi, 
      214062 China.
FAU - Bian, Zehua
AU  - Bian Z
AD  - Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, Wuxi, 
      214062 China.
FAU - Zhou, Jialiang
AU  - Zhou J
AD  - Department of Radiation Oncology, The Affiliated Hospital of Jiangnan University, 
      Wuxi, 214062 China.
FAU - Song, Mingxu
AU  - Song M
AD  - Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, Wuxi, 
      214062 China.
FAU - Liu, Zhihui
AU  - Liu Z
AD  - Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, Wuxi, 
      214062 China.
FAU - Feng, Yuyang
AU  - Feng Y
AD  - Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, Wuxi, 
      214062 China.
FAU - Zhe, Li
AU  - Zhe L
AD  - Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences, 
      Shanghai Medical College, Fudan University, Shanghai, 200032 China.
FAU - Zhang, Binbin
AU  - Zhang B
AD  - Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, Wuxi, 
      214062 China.
FAU - Yin, Yuan
AU  - Yin Y
AD  - Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, Wuxi, 
      214062 China.
FAU - Huang, Zhaohui
AU  - Huang Z
AD  - Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, Wuxi, 
      214062 China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150807
PL  - Germany
TA  - Protein Cell
JT  - Protein & cell
JID - 101532368
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MIRN638 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 3.1.4.4 (Phospholipase D)
RN  - EC 3.1.4.4 (phospholipase D1)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Apoptosis/genetics
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Down-Regulation/genetics
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Phospholipase D/*genetics
MH  - Prognosis
MH  - Stomach Neoplasms/diagnosis/enzymology/genetics/*pathology
PMC - PMC4537476
EDAT- 2015/08/08 06:00
MHDA- 2016/05/25 06:00
PMCR- 2015/08/07
CRDT- 2015/08/08 06:00
PHST- 2015/05/12 00:00 [received]
PHST- 2015/06/11 00:00 [accepted]
PHST- 2015/08/08 06:00 [entrez]
PHST- 2015/08/08 06:00 [pubmed]
PHST- 2016/05/25 06:00 [medline]
PHST- 2015/08/07 00:00 [pmc-release]
AID - 187 [pii]
AID - 10.1007/s13238-015-0187-8 [doi]
PST - ppublish
SO  - Protein Cell. 2015 Sep;6(9):680-688. doi: 10.1007/s13238-015-0187-8. Epub 2015 
      Aug 7.