PMID- 26251187
OWN - NLM
STAT- MEDLINE
DCOM- 20171120
LR  - 20200930
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Linking)
VI  - 104
IP  - 7
DP  - 2016 Oct
TI  - Cellobiose dehydrogenase functionalized urinary catheter as novel antibiofilm 
      system.
PG  - 1448-56
LID - 10.1002/jbm.b.33491 [doi]
AB  - Urinary catheters expose patients to a high risk of acquiring nosocomial 
      infections. To prevent this risk of infection, cellobiose dehydrogenase (CDH), an 
      antimicrobial enzyme able to use various oligosaccharides as electron donors to 
      produce hydrogen peroxide using oxygen as an electron acceptor, was covalently 
      grafted onto plasma-activated urinary polydimethylsiloxane (PDMS) catheter 
      surfaces. Successful immobilization of CDH on PDMS was confirmed by Fourier 
      transformed-infrared spectrometry and production of H2 O2 . The CDH 
      functionalized PDMS surfaces reduced the amount of viable Staphylococcus aureus 
      by 60%, total biomass deposited on the surface by 30% and 70% of biofilm 
      formation. The immobilized CDH was relatively stable in artificial urine over 16 
      days, retaining 20% of its initial activity. The CDH coated PDMS surface did not 
      affect the growth and physiology of HEK 239 and RAW 264,7 mammalian cells. 
      Therefore this new CDH functionalized catheter system shows great potential for 
      solving the current problems associated with urinary catheters. (c) 2015 Wiley 
      Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1448-1456, 
      2016.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Thallinger, Barbara
AU  - Thallinger B
AD  - Institute of Environmental Biotechnology, University of Natural Resources and 
      Life Sciences, 3430, Tulln, Austria. Barbara.Thallinger@boku.ac.at.
FAU - Brandauer, Martin
AU  - Brandauer M
AD  - Institute of Environmental Biotechnology, University of Natural Resources and 
      Life Sciences, 3430, Tulln, Austria.
FAU - Burger, Peter
AU  - Burger P
AD  - Institute of Environmental Biotechnology, University of Natural Resources and 
      Life Sciences, 3430, Tulln, Austria.
FAU - Sygmund, Christoph
AU  - Sygmund C
AD  - Department of Food Sciences and Technology, Food Biotechnology Laboratory, 
      University of Natural Resources and Life Sciences, 1190, Vienna, Austria.
FAU - Ludwig, Roland
AU  - Ludwig R
AD  - Department of Food Sciences and Technology, Food Biotechnology Laboratory, 
      University of Natural Resources and Life Sciences, 1190, Vienna, Austria.
FAU - Ivanova, Kristina
AU  - Ivanova K
AD  - Department d'Enginyeria Quimica, Universitat Politecnica de Catalunya, Grup de 
      Biotecnologia Molecular i Industrial, 08222, Terrassa, Spain.
FAU - Kun, Jutta
AU  - Kun J
AD  - Synovo GesmbH, 72076, Tubingen, Germany.
FAU - Scaini, Denis
AU  - Scaini D
AD  - Elettra Sincrotrone, 34149, Basovizza, TS, Italy.
FAU - Burnet, Michael
AU  - Burnet M
AD  - Synovo GesmbH, 72076, Tubingen, Germany.
FAU - Tzanov, Tzanko
AU  - Tzanov T
AD  - Department d'Enginyeria Quimica, Universitat Politecnica de Catalunya, Grup de 
      Biotecnologia Molecular i Industrial, 08222, Terrassa, Spain.
FAU - Nyanhongo, Gibson S
AU  - Nyanhongo GS
AD  - Institute of Environmental Biotechnology, University of Natural Resources and 
      Life Sciences, 3430, Tulln, Austria. g.nyanhongo@boku.ac.at.
AD  - Botswana International University of Science and Technology, Private Mail Bag 16, 
      Palapye Botswana. g.nyanhongo@boku.ac.at.
FAU - Guebitz, Georg M
AU  - Guebitz GM
AD  - Institute of Environmental Biotechnology, University of Natural Resources and 
      Life Sciences, 3430, Tulln, Austria.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150806
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Fungal Proteins)
RN  - 63148-62-9 (baysilon)
RN  - EC 1.1.- (Carbohydrate Dehydrogenases)
RN  - EC 1.1.99.18 (cellobiose-quinone oxidoreductase)
SB  - IM
MH  - Animals
MH  - Ascomycota/*enzymology
MH  - Biofilms/*growth & development
MH  - Carbohydrate Dehydrogenases/*chemistry
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Fungal Proteins/*chemistry
MH  - HEK293 Cells
MH  - Humans
MH  - Mice
MH  - RAW 264.7 Cells
MH  - Staphylococcus aureus/*physiology
MH  - *Urinary Catheters
OTO - NOTNLM
OT  - antimicrobial effect
OT  - biocompatibility
OT  - cellobiose dehydrogenase
OT  - covalent immobilization
OT  - polydimethylsiloxane
OT  - urinary catheters
EDAT- 2015/08/08 06:00
MHDA- 2017/11/29 06:00
CRDT- 2015/08/08 06:00
PHST- 2015/03/25 00:00 [received]
PHST- 2015/07/07 00:00 [revised]
PHST- 2015/07/18 00:00 [accepted]
PHST- 2015/08/08 06:00 [entrez]
PHST- 2015/08/08 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
AID - 10.1002/jbm.b.33491 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2016 Oct;104(7):1448-56. doi: 
      10.1002/jbm.b.33491. Epub 2015 Aug 6.