PMID- 26252002
OWN - NLM
STAT- MEDLINE
DCOM- 20160517
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 8
DP  - 2015
TI  - Interstitial Telomeric Motifs in Squamate Reptiles: When the Exceptions Outnumber 
      the Rule.
PG  - e0134985
LID - 10.1371/journal.pone.0134985 [doi]
LID - e0134985
AB  - Telomeres are nucleoprotein complexes protecting the physical ends of linear 
      eukaryotic chromosomes and therefore helping to ensure their stability and 
      integrity. Additionally, telomeric sequences can be localized in non-terminal 
      regions of chromosomes, forming so-called interstitial telomeric sequences 
      (ITSs). ITSs are traditionally considered to be relics of chromosomal 
      rearrangements and thus very informative in the reconstruction of the 
      evolutionary history of karyotype formation. We examined the distribution of the 
      telomeric motifs (TTAGGG)n using fluorescence in situ hybridization (FISH) in 30 
      species, representing 17 families of squamate reptiles, and compared them with 
      the collected data from another 38 species from literature. Out of the 68 
      squamate species analyzed, 35 possess ITSs in pericentromeric regions, 
      centromeric regions and/or within chromosome arms. We conclude that the 
      occurrence of ITSs is rather common in squamates, despite their generally 
      conserved karyotypes, suggesting frequent and independent cryptic chromosomal 
      rearrangements in this vertebrate group.
FAU - Rovatsos, Michail
AU  - Rovatsos M
AD  - Department of Ecology, Faculty of Science, Charles University in Prague, Prague, 
      128 44, Czech Republic.
FAU - Kratochvil, Lukas
AU  - Kratochvil L
AD  - Department of Ecology, Faculty of Science, Charles University in Prague, Prague, 
      128 44, Czech Republic.
FAU - Altmanova, Marie
AU  - Altmanova M
AD  - Department of Ecology, Faculty of Science, Charles University in Prague, Prague, 
      128 44, Czech Republic.
FAU - Johnson Pokorna, Martina
AU  - Johnson Pokorna M
AD  - Department of Ecology, Faculty of Science, Charles University in Prague, Prague, 
      128 44, Czech Republic; Institute of Animal Physiology and Genetics, The Academy 
      of Sciences of the Czech Republic, Libechov, 277 21, Czech Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150807
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Centromere/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Nucleotide Motifs/*genetics
MH  - Reptiles/*genetics
MH  - Telomere/*genetics
PMC - PMC4529230
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/08/08 06:00
MHDA- 2016/05/18 06:00
PMCR- 2015/08/07
CRDT- 2015/08/08 06:00
PHST- 2015/04/24 00:00 [received]
PHST- 2015/07/15 00:00 [accepted]
PHST- 2015/08/08 06:00 [entrez]
PHST- 2015/08/08 06:00 [pubmed]
PHST- 2016/05/18 06:00 [medline]
PHST- 2015/08/07 00:00 [pmc-release]
AID - PONE-D-15-15510 [pii]
AID - 10.1371/journal.pone.0134985 [doi]
PST - epublish
SO  - PLoS One. 2015 Aug 7;10(8):e0134985. doi: 10.1371/journal.pone.0134985. 
      eCollection 2015.