PMID- 26252418
OWN - NLM
STAT- MEDLINE
DCOM- 20160810
LR  - 20151008
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 6
IP  - 10
DP  - 2015 Oct
TI  - In vitro fermentation of lupin seeds (Lupinus albus) and broad beans (Vicia 
      faba): dynamic modulation of the intestinal microbiota and metabolomic output.
PG  - 3316-22
LID - 10.1039/c5fo00675a [doi]
AB  - Broad beans (Vicia faba) and lupin seeds (Lupinus albus) are legumes rich in a 
      wide range of compounds, which may represent a useful dietary approach for 
      modulating the human gut microbiome. In this work, after in vitro digestion, 
      legume samples were used as carbon sources in anaerobic batch cultures to 
      evaluate their impact on the intestinal microbiota composition and on their 
      metabolic products. The fermentations were monitored by a decrease in pH, 
      generation of short chain fatty acids (SCFA) and lactate and the changes in the 
      dynamic bacterial populations by fluorescence in situ hybridization (FISH). The 
      total SCFA at the end of fermentation was 81.52 mM for lupin seeds and 78.41 mM 
      for broad beans accompanied by a decrease of the pH for both legumes. The 
      microbial groups that increased significantly (P < 0.05) were Bifidobacterium 
      spp., Lactobacillus-Enterococcus, Atopobium, Bacteroides-Pretovella, Clostridium 
      coccoides-Eubacterium rectale, Faecalibacterium prausnitzii and Roseburia 
      intestinalis. This impact on the intestinal microbiota suggests that lupin seeds 
      and broad beans may be used in the development of novel functional foods, which 
      can be included in dietary strategies for human health promotion.
FAU - Gullon, Patricia
AU  - Gullon P
AD  - CBQF - Centro de Biotecnologia e Quimica Fina - Laboratorio Associado, Escola 
      Superior de Biotecnologia, Universidade Catolica Portuguesa/Porto, Rua Arquiteto 
      Lobao Vital, Apartado 2511, 4202-401 Porto, Portugal. beatriz.gullon@usc.es.
FAU - Gullon, Beatriz
AU  - Gullon B
FAU - Tavaria, Freni
AU  - Tavaria F
FAU - Vasconcelos, Marta
AU  - Vasconcelos M
FAU - Gomes, Ana Maria
AU  - Gomes AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Fatty Acids, Volatile)
RN  - 0 (Plant Preparations)
RN  - 0 (Prebiotics)
SB  - IM
MH  - Bacteroides/isolation & purification
MH  - Bifidobacterium/isolation & purification
MH  - Clostridium/isolation & purification
MH  - Fatty Acids, Volatile/metabolism
MH  - Feces/microbiology
MH  - Fermentation
MH  - *Gastrointestinal Microbiome
MH  - Gastrointestinal Tract/drug effects/microbiology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - In Situ Hybridization, Fluorescence
MH  - Lactobacillus/isolation & purification
MH  - Lupinus/*chemistry
MH  - Metabolomics
MH  - Plant Preparations/pharmacology
MH  - Prebiotics
MH  - Seeds/*chemistry
MH  - Vegetables
MH  - Vicia faba/*chemistry
EDAT- 2015/08/08 06:00
MHDA- 2016/08/11 06:00
CRDT- 2015/08/08 06:00
PHST- 2015/08/08 06:00 [entrez]
PHST- 2015/08/08 06:00 [pubmed]
PHST- 2016/08/11 06:00 [medline]
AID - 10.1039/c5fo00675a [doi]
PST - ppublish
SO  - Food Funct. 2015 Oct;6(10):3316-22. doi: 10.1039/c5fo00675a.