PMID- 26253107
OWN - NLM
STAT- MEDLINE
DCOM- 20160502
LR  - 20150808
IS  - 1559-4106 (Electronic)
IS  - 1559-4106 (Linking)
VI  - 11
IP  - 2
DP  - 2015 Jun 7
TI  - 3D chemical characterization of frozen hydrated hydrogels using ToF-SIMS with 
      argon cluster sputter depth profiling.
PG  - 02A301
LID - 10.1116/1.4928209 [doi]
AB  - Hydrogels have been used extensively in bioengineering as artificial cell culture 
      supports. Investigation of the interrelationship between cellular response to the 
      hydrogel and its chemistry ideally requires methods that allow characterization 
      without labels and can map species in three-dimensional to follow biomolecules 
      adsorbed to, and absorbed into, the open structure before and during culture. 
      Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has the potential to be 
      utilized for through thickness characterization of hydrogels. The authors have 
      established a simple sample preparation procedure to successfully achieve 
      analysis of frozen hydrated hydrogels using ToF-SIMS without the need for dry 
      glove box entry equipment. They demonstrate this on a poly(2-hydroxyethyl 
      methacrylate) (pHEMA) film where a model protein (lysozyme) is incorporated using 
      two methods to demonstrate how protein distribution can be determined. A 
      comparison of lysozyme incorporation is made between the situation where the 
      protein is present in a polymer dip coating solution and where lysozyme is in an 
      aqueous medium in which the film is incubated. It is shown that protonated water 
      clusters H(H2O)n (+) where n = 5-11 that are indicative of ice are detected 
      through the entire thickness of the pHEMA. The lysozyme distribution through the 
      pHEMA hydrogel films can be determined using the intensity of a characteristic 
      amino acid secondary ion fragment.
FAU - Taylor, Michael
AU  - Taylor M
AD  - School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 
      2RD, United Kingdom.
FAU - Scurr, David
AU  - Scurr D
AD  - School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 
      2RD, United Kingdom.
FAU - Lutolf, Matthias
AU  - Lutolf M
AD  - Laboratory of Stem Cell Bioengineering, Ecole Polytechnique Federale de Lausanne, 
      Lausanne CH-1015, Switzerland.
FAU - Buttery, Lee
AU  - Buttery L
AD  - School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 
      2RD, United Kingdom.
FAU - Zelzer, Mischa
AU  - Zelzer M
AD  - School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 
      2RD, United Kingdom.
FAU - Alexander, Morgan
AU  - Alexander M
AD  - School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 
      2RD, United Kingdom.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150607
PL  - United States
TA  - Biointerphases
JT  - Biointerphases
JID - 101275679
RN  - 0 (Hydrogels)
RN  - 0 (Proteins)
RN  - 67XQY1V3KH (Argon)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Argon
MH  - *Freezing
MH  - Hydrogels/*chemistry
MH  - Lasers
MH  - Muramidase/analysis
MH  - Proteins/*analysis
MH  - Spectrometry, Mass, Secondary Ion/*methods
EDAT- 2015/08/09 06:00
MHDA- 2016/05/03 06:00
CRDT- 2015/08/09 06:00
PHST- 2015/08/09 06:00 [entrez]
PHST- 2015/08/09 06:00 [pubmed]
PHST- 2016/05/03 06:00 [medline]
AID - 10.1116/1.4928209 [doi]
PST - epublish
SO  - Biointerphases. 2015 Jun 7;11(2):02A301. doi: 10.1116/1.4928209.