PMID- 26253681
OWN - NLM
STAT- MEDLINE
DCOM- 20160601
LR  - 20181113
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 81
IP  - 20
DP  - 2015 Oct
TI  - Changes in Microbial Biofilm Communities during Colonization of Sewer Systems.
PG  - 7271-80
LID - 10.1128/AEM.01538-15 [doi]
AB  - The coexistence of sulfate-reducing bacteria (SRB) and methanogenic archaea (MA) 
      in anaerobic biofilms developed in sewer inner pipe surfaces favors the 
      accumulation of sulfide (H2S) and methane (CH4) as metabolic end products, 
      causing severe impacts on sewerage systems. In this study, we investigated the 
      time course of H2S and CH4 production and emission rates during different stages 
      of biofilm development in relation to changes in the composition of microbial 
      biofilm communities. The study was carried out in a laboratory sewer pilot plant 
      that mimics a full-scale anaerobic rising sewer using a combination of process 
      data and molecular techniques (e.g., quantitative PCR [qPCR], denaturing gradient 
      gel electrophoresis [DGGE], and 16S rRNA gene pyrotag sequencing). After 2 weeks 
      of biofilm growth, H2S emission was notably high (290.7+/-72.3 mg S-H2S liter(-1) 
      day(-1)), whereas emissions of CH4 remained low (17.9+/-15.9 mg COD-CH4 liter(-1) 
      day(-1)). This contrasting trend coincided with a stable SRB community and an 
      archaeal community composed solely of methanogens derived from the human gut 
      (i.e., Methanobrevibacter and Methanosphaera). In turn, CH4 emissions increased 
      after 1 year of biofilm growth (327.6+/-16.6 mg COD-CH4 liter(-1) day(-1)), 
      coinciding with the replacement of methanogenic colonizers by species more 
      adapted to sewer conditions (i.e., Methanosaeta spp.). Our study provides data 
      that confirm the capacity of our laboratory experimental system to mimic the 
      functioning of full-scale sewers both microbiologically and operationally in 
      terms of sulfide and methane production, gaining insight into the complex 
      dynamics of key microbial groups during biofilm development.
CI  - Copyright (c) 2015, American Society for Microbiology. All Rights Reserved.
FAU - Auguet, O
AU  - Auguet O
AD  - Catalan Institute for Water Research (ICRA), Girona, Spain.
FAU - Pijuan, M
AU  - Pijuan M
AD  - Catalan Institute for Water Research (ICRA), Girona, Spain.
FAU - Batista, J
AU  - Batista J
AD  - Catalan Institute for Water Research (ICRA), Girona, Spain.
FAU - Borrego, C M
AU  - Borrego CM
AUID- ORCID: 0000-0002-2708-3753
AD  - Catalan Institute for Water Research (ICRA), Girona, Spain Group of Molecular 
      Microbial Ecology, Institute of Aquatic Ecology, University of Girona, Girona, 
      Spain cborrego@icra.cat.
FAU - Gutierrez, O
AU  - Gutierrez O
AD  - Catalan Institute for Water Research (ICRA), Girona, Spain.
LA  - eng
SI  - BioProject/PRJNA279227
SI  - GENBANK/KR080151
SI  - GENBANK/KR080152
SI  - GENBANK/KR080153
SI  - GENBANK/KR080154
SI  - GENBANK/KR080155
SI  - GENBANK/KR080156
SI  - GENBANK/KR080157
SI  - GENBANK/KR080158
SI  - GENBANK/KR080159
SI  - GENBANK/KR080160
SI  - GENBANK/KR080161
SI  - GENBANK/KR080162
SI  - GENBANK/KR080163
SI  - GENBANK/KR080164
SI  - GENBANK/KR080165
SI  - GENBANK/KR080166
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150807
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 0 (Sulfates)
RN  - 0 (Sulfides)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Anaerobiosis
MH  - Archaea/*metabolism
MH  - Bacteria/*metabolism
MH  - Biofilms/*growth & development
MH  - Methane/metabolism
MH  - Molecular Sequence Data
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
MH  - Sulfates/metabolism
MH  - Sulfides/metabolism
PMC - PMC4579435
EDAT- 2015/08/09 06:00
MHDA- 2016/06/02 06:00
PMCR- 2016/04/01
CRDT- 2015/08/09 06:00
PHST- 2015/05/08 00:00 [received]
PHST- 2015/08/01 00:00 [accepted]
PHST- 2015/08/09 06:00 [entrez]
PHST- 2015/08/09 06:00 [pubmed]
PHST- 2016/06/02 06:00 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - AEM.01538-15 [pii]
AID - 01538-15 [pii]
AID - 10.1128/AEM.01538-15 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2015 Oct;81(20):7271-80. doi: 10.1128/AEM.01538-15. Epub 
      2015 Aug 7.