PMID- 26254362
OWN - NLM
STAT- MEDLINE
DCOM- 20160428
LR  - 20150808
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 35
IP  - 9
DP  - 2015 Sep
TI  - Apoptotic Effects of Novel Dithiocarbamate Analogs of Emetine in Prostate Cancer 
      Cell Lines.
PG  - 4723-32
AB  - BACKGROUND/AIM: Prostate cancer is one of the leading causes of death in American 
      males. Emetine, a naturally-derived alkaloid from the Ipecacuanha plant, has been 
      shown to have potential for anti-tumorigenic effects for cancer treatments. The 
      objective of this study was to characterize novel emetine dithiocarbamate 
      (EMTDTC) analogs for potent anti-tumorigenic activity with minimal toxicity to 
      normal prostate cells and identify targeted apoptotic regulatory genes. The 
      leading key compounds, EMTDTC-55 and EMTDTC-56 were studied. MATERIALS AND 
      METHODS: Established methods of cell flow cytometry were used to analyze 
      apoptotic potential in prostate cancer cell lines (DU145, PC3 and LNCaP) and real 
      time-polymerase chain reaction (PCR) for identifying key genes mediating 
      apoptosis. RESULTS: The effect of EMTDTC-55 on DU145, LNCaP and PC3 revealed 
      significant anti-tumorigenic activities. Both compounds showed highly significant 
      apoptotic potential on days 3 and 5 in the prostate cancer cells. Key apoptotic 
      genes were differentially regulated suggestive of cell-cycle arrest and apoptotic 
      induction in androgen-independent cell lines, DU145 and PC3, by both compounds. 
      However, in the androgen-dependent cell line LNCaP, cells were marginally 
      affected by EMTDTC-55, but significant apoptosis was observed by EMTDTC-56 
      leading to cell-cycle arrest. CONCLUSION: Both dithiocarbamate compounds 
      EMTDTC-55 and EMTDTC-56 have significant chemotherapeutic potential in moderately 
      metastatic DU145 and highly metastatic PC3 cells.
CI  - Copyright(c) 2015 International Institute of Anticancer Research (Dr. John G. 
      Delinassios), All rights reserved.
FAU - Bamji, Zebalda D
AU  - Bamji ZD
AD  - Division of Medical Genetics, Department of Pediatrics and Child Health, College 
      of Medicine, Howard University, Washington, DC, U.S.A.
FAU - Washington, Kareem N
AU  - Washington KN
AD  - Division of Medical Genetics, Department of Pediatrics and Child Health, College 
      of Medicine, Howard University, Washington, DC, U.S.A.
FAU - Akinboye, Emmanuel
AU  - Akinboye E
AD  - Department of Chemistry, Graduate School, Howard University, Washington, D.C, 
      U.S.A.
FAU - Bakare, Oladapo
AU  - Bakare O
AD  - Department of Chemistry, Graduate School, Howard University, Washington, D.C, 
      U.S.A.
FAU - Kanaan, Yasmine M
AU  - Kanaan YM
AD  - Department of Microbiology, College of Medicine, Howard University, Washington, 
      DC, U.S.A.
FAU - Copeland, Robert L Jr
AU  - Copeland RL Jr
AD  - Department of Pharmacology, College of Medicine, Howard University, Washington, 
      DC, U.S.A. rlcopeland@howard.edu.
LA  - eng
GR  - 2U54 CA091431-06/CA/NCI NIH HHS/United States
GR  - G12MD007597/MD/NIMHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 99Z2744345 (Ditiocarb)
RN  - X8D5EPO80M (Emetine)
SB  - IM
MH  - Apoptosis/*drug effects/genetics
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Ditiocarb/*analogs & derivatives/*pharmacology
MH  - Emetine/*pharmacology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - Male
MH  - Prostatic Neoplasms/genetics/*pathology
OTO - NOTNLM
OT  - Prostate Cancer
OT  - apoptosis
OT  - chemotherapeutic agents
OT  - dithiocarbamate analogs
OT  - emetine gene regulation
EDAT- 2015/08/09 06:00
MHDA- 2016/04/29 06:00
CRDT- 2015/08/09 06:00
PHST- 2015/08/09 06:00 [entrez]
PHST- 2015/08/09 06:00 [pubmed]
PHST- 2016/04/29 06:00 [medline]
AID - 35/9/4723 [pii]
PST - ppublish
SO  - Anticancer Res. 2015 Sep;35(9):4723-32.