PMID- 26254785
OWN - NLM
STAT- MEDLINE
DCOM- 20160526
LR  - 20150907
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 99
IP  - 19
DP  - 2015 Oct
TI  - Adaptation of acidogenic sludge to increasing glycerol concentrations for 
      biohydrogen production.
PG  - 8295-308
LID - 10.1007/s00253-015-6832-6 [doi]
AB  - Hydrogen is a promising alternative as an energetic carrier and its production by 
      dark fermentation from wastewater has been recently proposed, with special 
      attention to crude glycerol as potential substrate. In this study, two different 
      feeding strategies were evaluated for replacing the glucose substrate by glycerol 
      substrate: a one-step strategy (glucose was replaced abruptly by glycerol) and a 
      step-by-step strategy (progressive decrease of glucose concentration and increase 
      of glycerol concentration from 0 to 5 g L(-1)), in a continuous stirred tank 
      reactor (12 h of hydraulic retention time (HRT), pH 5.5, 35  degrees C). While the 
      one-step strategy led to biomass washout and unsuccessful H2 production, the 
      step-by-step strategy was efficient for biomass adaptation, reaching acceptable 
      hydrogen yields (0.4 +/- 0.1 molH2 mol(-1) glycerol consumed) around 33 % of the 
      theoretical yield independently of the glycerol concentration. Microbial 
      community structure was investigated by single-strand conformation polymorphism 
      (SSCP) and denaturing gradient gel electrophoresis (DGGE) fingerprinting 
      techniques, targeting either the total community (16S ribosomal RNA (rRNA) gene) 
      or the functional Clostridium population involved in H2 production (hydA gene), 
      as well as by 454 pyrosequencing of the total community. Multivariate analysis of 
      fingerprinting and pyrosequencing results revealed the influence of the feeding 
      strategy on the bacterial community structure and suggested the progressive 
      structural adaptation of the community to increasing glycerol concentrations, 
      through the emergence and selection of specific species, highly correlated to 
      environmental parameters. Particularly, this work highlighted an interesting 
      shift of dominant community members (putatively responsible of hydrogen 
      production in the continuous stirred tank reactor (CSTR)) according to the 
      gradient of glycerol proportion in the feed, from the family Veillonellaceae to 
      the genera Prevotella and Clostridium sp., putatively responsible of hydrogen 
      production in the CSTR.
FAU - Tapia-Venegas, E
AU  - Tapia-Venegas E
AD  - Escuela de Ingenieria Bioquimica, Pontificia Universidad Catolica de Valparaiso, 
      Avenida Brasil 2085, Valparaiso, Chile, estela.tapia.v@mail.pucv.cl.
FAU - Cabrol, L
AU  - Cabrol L
FAU - Brandhoff, B
AU  - Brandhoff B
FAU - Hamelin, J
AU  - Hamelin J
FAU - Trably, E
AU  - Trably E
FAU - Steyer, J P
AU  - Steyer JP
FAU - Ruiz-Filippi, G
AU  - Ruiz-Filippi G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150809
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Acids)
RN  - 0 (Sewage)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - PDC6A3C0OX (Glycerol)
SB  - IM
MH  - Acids/metabolism
MH  - Bacteria/classification/genetics/isolation & purification/*metabolism
MH  - Bioreactors/microbiology
MH  - Fermentation
MH  - Glycerol/analysis/*metabolism
MH  - Hydrogen/*metabolism
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Sewage/chemistry/*microbiology
EDAT- 2015/08/10 06:00
MHDA- 2016/05/27 06:00
CRDT- 2015/08/10 06:00
PHST- 2015/04/29 00:00 [received]
PHST- 2015/07/09 00:00 [accepted]
PHST- 2015/07/05 00:00 [revised]
PHST- 2015/08/10 06:00 [entrez]
PHST- 2015/08/10 06:00 [pubmed]
PHST- 2016/05/27 06:00 [medline]
AID - 10.1007/s00253-015-6832-6 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2015 Oct;99(19):8295-308. doi: 
      10.1007/s00253-015-6832-6. Epub 2015 Aug 9.