PMID- 26255968
OWN - NLM
STAT- MEDLINE
DCOM- 20151112
LR  - 20210227
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 465
IP  - 2
DP  - 2015 Sep 18
TI  - High-density lipoprotein-mediated transcellular cholesterol transport in mouse 
      aortic endothelial cells.
PG  - 256-61
LID - S0006-291X(15)30401-0 [pii]
LID - 10.1016/j.bbrc.2015.08.011 [doi]
AB  - Accumulation of unesterified cholesterol-rich lipid vesicles in the 
      subendothelial space contributes to atherogenesis. Transport of cholesterol from 
      the subendothelial intima back to the circulating blood inhibits atherosclerosis 
      development; however, the mechanism for this process has not been fully defined. 
      Using cultured mouse aortic endothelial cells (MAECs), we observed that 
      unesterified cholesterol can be transported across the endothelial cell monolayer 
      from the basolateral to the apical compartment. Administration of high-density 
      lipoprotein (HDL) or apolipoprotein AI (apoAI) to the apical compartment enhanced 
      transendothelial cholesterol transport in a concentration-dependent manner. 
      Knockdown of ATP-binding cassette transporter G1 (ABCG1) or scavenger receptor 
      class B type I (SR-B1), or inhibition of SR-B1 diminished HDL-induced 
      transendothelial cholesterol transport; while knockdown of ABCA1 reduced 
      apoAI-mediated cholesterol transport. HDL enhanced phosphorylation of 
      phosphatidylinositol 3-kinase (PI3K) and Akt in MAECs. However, inhibition of 
      PI3K or Akt did not reduce HDL-induced transendothelial cholesterol transport. 
      These results suggest that HDL enhances transendothelial cholesterol transport by 
      activation of a mechanism involving ABCA1, ABCG1 and SR-B1 but not involving PI3K 
      and Akt.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Miao, LiXia
AU  - Miao L
AD  - Department of Physiology, Meharry Medical College, Nashville, TN 37208, USA; 
      Department of Biochemistry, School of Basic Medical Sciences, Wuhan University, 
      Wuhan 430071, China.
FAU - Okoro, Emmanuel U
AU  - Okoro EU
AD  - Department of Physiology, Meharry Medical College, Nashville, TN 37208, USA.
FAU - Cao, ZhiJan
AU  - Cao Z
AD  - Department of Physiology, Meharry Medical College, Nashville, TN 37208, USA; 
      Department of Biochemistry, School of Basic Medical Sciences, Wuhan University, 
      Wuhan 430071, China.
FAU - Yang, Hong
AU  - Yang H
AD  - Department of Physiology, Meharry Medical College, Nashville, TN 37208, USA.
FAU - Motley-Johnson, Evangeline
AU  - Motley-Johnson E
AD  - Department of Physiology, Meharry Medical College, Nashville, TN 37208, USA.
FAU - Guo, Zhongmao
AU  - Guo Z
AD  - Department of Physiology, Meharry Medical College, Nashville, TN 37208, USA. 
      Electronic address: zguo@mmc.edu.
LA  - eng
GR  - SC1HL101431/HL/NHLBI NIH HHS/United States
GR  - UL1 TR000445/TR/NCATS NIH HHS/United States
GR  - G12 MD007586/MD/NIMHD NIH HHS/United States
GR  - G12MD007586/MD/NIMHD NIH HHS/United States
GR  - SC1 HL101431/HL/NHLBI NIH HHS/United States
GR  - U54MD0007593/MD/NIMHD NIH HHS/United States
GR  - U54 MD007593/MD/NIMHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150807
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (ABCA1 protein, mouse)
RN  - 0 (ABCG1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Apolipoprotein A-I)
RN  - 0 (Lipoproteins)
RN  - 0 (Lipoproteins, HDL)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Scarb1 protein, mouse)
RN  - 0 (Scavenger Receptors, Class B)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - ATP Binding Cassette Transporter 1/antagonists & inhibitors/genetics/*metabolism
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/antagonists & inhibitors/genetics/*metabolism
MH  - Animals
MH  - Aorta/cytology/*drug effects/metabolism
MH  - Apolipoprotein A-I/metabolism/pharmacology
MH  - Biological Transport/drug effects
MH  - Cell Polarity
MH  - Dose-Response Relationship, Drug
MH  - Endothelial Cells/cytology/*drug effects/metabolism
MH  - Gene Expression
MH  - Lipoproteins/antagonists & inhibitors/genetics/*metabolism
MH  - Lipoproteins, HDL/metabolism/*pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phosphatidylinositol 3-Kinase/genetics/metabolism
MH  - Phosphorylation/drug effects
MH  - Primary Cell Culture
MH  - Proto-Oncogene Proteins c-akt/genetics/metabolism
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Scavenger Receptors, Class B/antagonists & inhibitors/genetics/*metabolism
PMC - PMC4576456
MID - NIHMS715777
OTO - NOTNLM
OT  - ATP-binding cassette transporter
OT  - High-density lipoprotein
OT  - Scavenger receptor class B type I
OT  - Transendothelial cholesterol transport
COIS- Conflict of Interest The authors have no conflicts to disclose.
EDAT- 2015/08/11 06:00
MHDA- 2015/11/13 06:00
PMCR- 2016/09/18
CRDT- 2015/08/11 06:00
PHST- 2015/07/27 00:00 [received]
PHST- 2015/08/03 00:00 [accepted]
PHST- 2015/08/11 06:00 [entrez]
PHST- 2015/08/11 06:00 [pubmed]
PHST- 2015/11/13 06:00 [medline]
PHST- 2016/09/18 00:00 [pmc-release]
AID - S0006-291X(15)30401-0 [pii]
AID - 10.1016/j.bbrc.2015.08.011 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Sep 18;465(2):256-61. doi: 
      10.1016/j.bbrc.2015.08.011. Epub 2015 Aug 7.