PMID- 26260512 OWN - NLM STAT- MEDLINE DCOM- 20160809 LR - 20151014 IS - 1932-8494 (Electronic) IS - 1932-8486 (Linking) VI - 298 IP - 11 DP - 2015 Nov TI - Interstitial Outburst of Angiogenic Factors During Skeletal Muscle Regeneration After Acute Mechanical Trauma. PG - 1864-79 LID - 10.1002/ar.23254 [doi] AB - Angiogenesis is a key event during tissue regeneration, but the intimate mechanisms controlling this process are still largely unclear. Therefore, the cellular and molecular interplay along normal tissue regeneration should be carefully unveiled. To this matter, we investigated by xMAP assay the dynamics of some angiogenic factors known to be involved in tissue repair, such as follistatin (FST), Placental Growth Factor-2 (PLGF-2), epidermal growth factor (EGF), betacellulin (BTC), and amphiregulin (AREG) using an animal model that mimics acute muscle contusion injuries. In situ immunofluorescence was used for the evaluation and tissue distribution of their cellular sources. Tissue levels of explored factors increased significantly during degeneration and inflammatory stage of regeneration, peaking first week postinjury. However, except for PLGF-2 and EGF, their levels remained significantly elevated after the inflammatory process started to fade. Serum levels were significantly increased only after 24 h for AREG and EGF. Though, for all factors except FST, the levels in injured samples did not correlate with serum or contralateral tissue levels, excluding the systemic influence. We found significant correlations between the levels of EGF and AREG, BTC, FST and FST and AREG in injured samples. Interstitial cells expressing these factors were highlighted by in situ immunolabeling and their number correlated with measured levels dynamics. Our study provides evidence of a dynamic level variation along the regeneration process and a potential interplay between selected angiogenic factors. They are synthesized, at least partially, by cell populations residing in skeletal muscle interstitium during regeneration after acute muscle trauma. CI - (c) 2015 Wiley Periodicals, Inc. FAU - Ceafalan, Laura Cristina AU - Ceafalan LC AD - Department of Cellular and Molecular Biology and Histology, School of Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania. AD - Department of Molecular Medicine and Neuroscience, "Victor Babes" Institute of Pathology, Bucharest, Romania. FAU - Manole, Emilia AU - Manole E AD - Department of Molecular Medicine and Neuroscience, "Victor Babes" Institute of Pathology, Bucharest, Romania. FAU - Tanase, Cristiana Pistol AU - Tanase CP AD - Biochemistry/Proteomics Department, "Victor Babes" Institute of Pathology, Bucharest, Romania. FAU - Codrici, Elena AU - Codrici E AD - Biochemistry/Proteomics Department, "Victor Babes" Institute of Pathology, Bucharest, Romania. FAU - Mihai, Simona AU - Mihai S AD - Biochemistry/Proteomics Department, "Victor Babes" Institute of Pathology, Bucharest, Romania. FAU - Gonzalez, Aldebarani AU - Gonzalez A AD - Department of Cellular and Molecular Biology and Histology, School of Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania. FAU - Popescu, Bogdan Ovidiu AU - Popescu BO AD - Department of Molecular Medicine and Neuroscience, "Victor Babes" Institute of Pathology, Bucharest, Romania. AD - Department of Neurology, Colentina Clinical Hospital-Colentina Research Center, School of Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150827 PL - United States TA - Anat Rec (Hoboken) JT - Anatomical record (Hoboken, N.J. : 2007) JID - 101292775 RN - 0 (Angiogenesis Inducing Agents) SB - IM MH - Acute Disease MH - Angiogenesis Inducing Agents/*metabolism MH - Animals MH - Fluorescent Antibody Technique MH - Mice MH - Muscle, Skeletal/*cytology/injuries/*metabolism MH - Wounds and Injuries/*metabolism/pathology OTO - NOTNLM OT - EGF OT - amphiregulin OT - angiogenic factors OT - betacellulin OT - follistatin OT - interstitial cells OT - placental growth factor-2 OT - skeletal muscle regeneration EDAT- 2015/08/12 06:00 MHDA- 2016/08/10 06:00 CRDT- 2015/08/12 06:00 PHST- 2015/02/09 00:00 [received] PHST- 2015/06/01 00:00 [revised] PHST- 2015/06/12 00:00 [accepted] PHST- 2015/08/12 06:00 [entrez] PHST- 2015/08/12 06:00 [pubmed] PHST- 2016/08/10 06:00 [medline] AID - 10.1002/ar.23254 [doi] PST - ppublish SO - Anat Rec (Hoboken). 2015 Nov;298(11):1864-79. doi: 10.1002/ar.23254. Epub 2015 Aug 27.