PMID- 26261538
OWN - NLM
STAT- MEDLINE
DCOM- 20160610
LR  - 20211203
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 8
IP  - 6
DP  - 2015
TI  - Impact of E6-associated protein on the proliferation and invasion of prostate 
      cancer cells in bone metastasis.
PG  - 6571-5
AB  - PURPOSE: To understand E6 associated protein (E6-AP)'s influence on prostate 
      cancer cell proliferation and infiltration, thus providing the theoretical basis 
      for developing therapeutic drugs for prostate cancer metastasis to the bone. 
      METHODS: Electroporation was performed to introduce linear regulatory plasmid 
      PrevTet-off-in and conjugative plasmid PrevTRE2-flag-E6AP into prostate cancer 
      cell line to establish wild-type E6-AP over-expressing transgenic LNCaP cell 
      line; Western blot assay was adopted to examine expression levels of E6-AP, 
      mammalian target of rapamycin (mTOR), protein kinase B (Akt), and 
      phosphoinositide 3-kinase (PI3K); PI3K inhibitor LY294002 was applied to all the 
      cells and MTT assay was used to measure cell proliferation; Matrigel invasion 
      chamber assay was adopted to detect cancer cell migration and invasion. RESULTS: 
      Stably transfected LNCaP cells that over expressed E6-AP had higher expression 
      levels of PI3K, Akt, and mTOR than control LNCaP cells; MTT assay showed that 
      E6-AP-LNCaP cells were more responsive to the inhibitory effect of LY294002; 
      Matrigel invasion chamber assay revealed increased cell crawling and adhesiveness 
      of E6-AP-LNCaP cells. CONCLUSION: Stable over-expression of E6-AP increases the 
      proliferation and invasion of LNCaP cells.
FAU - Zhang, Liyan
AU  - Zhang L
AD  - Department of Orthopedics, General Hospital of Chinese PLA Beijing 100853, China 
      ; First Department of Orthopaedics, Affiliated Hospital of Beihua University 
      Jilin 132000, Jilin Province, China.
FAU - Hu, Xiaoguang
AU  - Hu X
AD  - First Department of Orthopaedics, Affiliated Hospital of Beihua University Jilin 
      132000, Jilin Province, China.
FAU - Chen, Jiying
AU  - Chen J
AD  - Department of Orthopedics, General Hospital of Chinese PLA Beijing 100853, China.
FAU - Fu, Guilian
AU  - Fu G
AD  - Department of Molecular Biology of Beihua University Jilin 132013, Jilin 
      Province, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150601
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Protein Kinase Inhibitors)
RN  - EC 2.3.2.26 (UBE3A protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Bone Neoplasms/*enzymology/genetics/*secondary
MH  - Cell Line, Tumor
MH  - *Cell Movement/drug effects
MH  - *Cell Proliferation/drug effects
MH  - Enzyme Induction
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - Neoplasm Invasiveness
MH  - Phosphatidylinositol 3-Kinase/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Phosphorylation
MH  - Prostatic Neoplasms/*enzymology/genetics/*pathology
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Time Factors
MH  - Transfection
MH  - Ubiquitin-Protein Ligases/*biosynthesis/genetics
MH  - Up-Regulation
PMC - PMC4525872
OTO - NOTNLM
OT  - E6-AP
OT  - Prostate cancer
OT  - invasion
OT  - proliferation
EDAT- 2015/08/12 06:00
MHDA- 2016/06/11 06:00
PMCR- 2015/06/01
CRDT- 2015/08/12 06:00
PHST- 2015/03/26 00:00 [received]
PHST- 2015/05/20 00:00 [accepted]
PHST- 2015/08/12 06:00 [entrez]
PHST- 2015/08/12 06:00 [pubmed]
PHST- 2016/06/11 06:00 [medline]
PHST- 2015/06/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2015 Jun 1;8(6):6571-5. eCollection 2015.