PMID- 26263178
OWN - NLM
STAT- MEDLINE
DCOM- 20160510
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 8
DP  - 2015
TI  - Efficient Generation of Plasmacytoid Dendritic Cell from Common Lymphoid 
      Progenitors by Flt3 Ligand.
PG  - e0135217
LID - 10.1371/journal.pone.0135217 [doi]
LID - e0135217
AB  - Dendritic cells (DCs), including conventional DCs (cDCs) and plasmacytoid DCs 
      (pDCs) are critical for initiating and controlling the immune response. However, 
      study of DC, particularly pDC, function is hampered by their low frequency in 
      lymphoid organs, and existing methods for in vitro DC generation preferentially 
      favor the production of cDCs over pDCs. Here, we demonstrated that pDCs could be 
      efficiently generated in vitro from common lymphoid progenitors (CLPs) using Flt3 
      ligand (FL) in three different culture systems, namely feeder-free, BM-feeder and 
      AC-6-feeder. This was in stark contrast to common DC progenitors (CDPs), in which 
      cDCs were prominently generated under the same conditions. Moreover, the 
      efficiency and function of pDCs generated from these three systems varied. While 
      AC-6 system showed the greatest ability to support pDC development from CLPs, 
      BM-feeder system was able to develop pDCs with better functionality. pDCs could 
      also be expanded in vivo using hydrodynamic gene transfer of FL, which was 
      further enhanced by the combined treatment of FL and IFN-alpha. Interestingly, IFN-alpha 
      selectively promoted the proliferation of CLPs and not CDPs, which might 
      contribute to enhanced pDC development. Together, we have defined conditions for 
      in vitro and in vivo generation of pDCs, which may be useful for investigating 
      the biology of pDCs.
FAU - Chen, Yi-Ling
AU  - Chen YL
AD  - Graduate Institute of Immunology, National Taiwan University College of Medicine, 
      Taipei, Taiwan.
FAU - Chang, Shiun
AU  - Chang S
AD  - Graduate Institute of Immunology, National Taiwan University College of Medicine, 
      Taipei, Taiwan.
FAU - Chen, Ting-Ting
AU  - Chen TT
AD  - Graduate Institute of Immunology, National Taiwan University College of Medicine, 
      Taipei, Taiwan.
FAU - Lee, Chien-Kuo
AU  - Lee CK
AD  - Graduate Institute of Immunology, National Taiwan University College of Medicine, 
      Taipei, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150811
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Interferon Type I)
RN  - 0 (Membrane Proteins)
RN  - 0 (flt3 ligand protein)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*cytology/*drug effects/metabolism
MH  - Interferon Type I/biosynthesis
MH  - Lymphoid Progenitor Cells/*cytology/*drug effects/metabolism
MH  - Membrane Proteins/*pharmacology
MH  - Mice
PMC - PMC4532451
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/08/12 06:00
MHDA- 2016/05/11 06:00
PMCR- 2015/08/11
CRDT- 2015/08/12 06:00
PHST- 2014/07/14 00:00 [received]
PHST- 2015/07/20 00:00 [accepted]
PHST- 2015/08/12 06:00 [entrez]
PHST- 2015/08/12 06:00 [pubmed]
PHST- 2016/05/11 06:00 [medline]
PHST- 2015/08/11 00:00 [pmc-release]
AID - PONE-D-14-31520 [pii]
AID - 10.1371/journal.pone.0135217 [doi]
PST - epublish
SO  - PLoS One. 2015 Aug 11;10(8):e0135217. doi: 10.1371/journal.pone.0135217. 
      eCollection 2015.