PMID- 26263972
OWN - NLM
STAT- MEDLINE
DCOM- 20160509
LR  - 20181113
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 16
IP  - 8
DP  - 2015 Jul 30
TI  - Development of Ophiocordyceps sinensis through Plant-Mediated Interkingdom Host 
      Colonization.
PG  - 17482-93
LID - 10.3390/ijms160817482 [doi]
AB  - Ophiocordyceps sinensis is a well-known entomogenous and medicinal fungus. After 
      its anamorphs parasitize the larvae of the genus Thitarodes, fruit-bodies may 
      form to be used as medicine. However, its developmental mechanisms remain 
      unknown. The distribution of O. sinensis was determined in different tissues of 
      the Thitarodes larvae and the dominant plant species using real-time quantitative 
      polymerase chain reaction (qPCR) and fluorescence in situ hybridization (FISH) 
      technique, respectively. We found that more fungal material was located in plants 
      than in larvae, especially in Ranunculus tanguticus. A considerable amount was 
      detected in larval intestinal-wall and plant roots. It is suggested that plants 
      are the potential hosts of O. sinensis, which modifies our understanding of the 
      life cycle of O. sinensis and indicates that the phytophagous larvae may become 
      infected as they feed. Our research may contribute to the study of systematic 
      evolution and population ecology of O. sinensis, elucidate its developmental 
      mechanism and promote sustainable harvesting.
FAU - Lei, Wei
AU  - Lei W
AD  - Laboratory of Cardiovascular Diseases, Guangdong Medical College, Zhanjiang 
      524001, China. leiwei2006@126.com.
AD  - Food and Health Engineering Research Center of State Education Ministry, School 
      of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. 
      leiwei2006@126.com.
FAU - Zhang, Guren
AU  - Zhang G
AD  - Food and Health Engineering Research Center of State Education Ministry, School 
      of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. 
      zhanggr@mail.sysu.edu.cn.
FAU - Peng, Qingyun
AU  - Peng Q
AD  - Food and Health Engineering Research Center of State Education Ministry, School 
      of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. 
      p287931475@126.com.
FAU - Liu, Xin
AU  - Liu X
AD  - Food and Health Engineering Research Center of State Education Ministry, School 
      of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. 
      lsslx@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150730
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
MH  - Animals
MH  - *Host-Parasite Interactions
MH  - Hypocreales/genetics/*growth & development/pathogenicity
MH  - Larva/genetics
MH  - Lepidoptera/growth & development/*microbiology
MH  - Plant Roots/genetics/microbiology
MH  - Ranunculus/microbiology
MH  - Tissue Distribution/*genetics
PMC - PMC4581204
OTO - NOTNLM
OT  - Ophiocordyceps sinensis
OT  - caterpillar
OT  - development
OT  - interkingdom colonization
OT  - plant
EDAT- 2015/08/13 06:00
MHDA- 2016/05/10 06:00
PMCR- 2015/08/01
CRDT- 2015/08/13 06:00
PHST- 2015/06/15 00:00 [received]
PHST- 2015/07/15 00:00 [revised]
PHST- 2015/07/22 00:00 [accepted]
PHST- 2015/08/13 06:00 [entrez]
PHST- 2015/08/13 06:00 [pubmed]
PHST- 2016/05/10 06:00 [medline]
PHST- 2015/08/01 00:00 [pmc-release]
AID - ijms160817482 [pii]
AID - ijms-16-17482 [pii]
AID - 10.3390/ijms160817482 [doi]
PST - epublish
SO  - Int J Mol Sci. 2015 Jul 30;16(8):17482-93. doi: 10.3390/ijms160817482.