PMID- 26265453
OWN - NLM
STAT- MEDLINE
DCOM- 20160321
LR  - 20240323
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 11
DP  - 2015 Aug 12
TI  - Inhibition of porcine reproductive and respiratory syndrome virus replication in 
      vitro using DNA-based short antisense oligonucleotides.
PG  - 199
LID - 10.1186/s12917-015-0518-2 [doi]
LID - 199
AB  - BACKGROUND: Porcine reproductive and respiratory syndrome (PRRS) is caused by 
      porcine reproductive and respiratory syndrome virus (PRRSV) and is an 
      economically important disease in swine-producing areas. The objective of this 
      study was to screen for effective antisense oligonucleotides (AS-ONs) which could 
      inhibit PRRSV replication in MARC-145 cells and in pulmonary alveolar macrophages 
      (PAM). RESULTS: Nine short AS-ON sequences against the well-conserved regions of 
      PRRSV (5'-UTR, NSP9, ORF5 and ORF7) were selected. When MARC-145 cells or PAM 
      were infected with PRRSV followed by transfection with AS-ONs, four AS-ON 
      sequences targeting 5'-UTR, ORF5 or NSP9 were found to be the most effective 
      oligonucleotides in decreasing the cytopathic effect (CPE) induced by PRRSV 
      infection. Quantitative PCR and indirect immunofluorescence staining confirmed 
      that ORF7 levels were significantly reduced both at RNA and protein levels. The 
      PRRSV titration data furthermore indicated that transfection with AS-ON YN8 could 
      reduce the PRRSV titer by 1000-fold compared with controls. CONCLUSION: The 
      results presented here indicate that DNA-based antisense oligonucleotides can 
      effectively inhibit PRRSV replication in MARC-145 cells and in PAM. Furthermore, 
      comparing with the reported hit rates (approximately 10-30 %), we achieved a 
      higher success rate (44 %). The strategy we took to design the antisense 
      sequences might be applied to select AS-ONs that more efficiently reduce the 
      expression of target genes.
FAU - Zheng, Longlong
AU  - Zheng L
AD  - Department of Veterinary Medicine, College of Animal Science and Technology, 
      Yunnan Agricultural University, Kunming, China. 18314575103@163.com.
FAU - Li, Xiang
AU  - Li X
AD  - Present address: Wulan Institute for Animal Health, Lingyuan, Chaoyang City, 
      Liaoning province, China. wlbjds22@126.com.
FAU - Zhu, Lingyun
AU  - Zhu L
AD  - Department of Veterinary Medicine, College of Animal Science and Technology, 
      Yunnan Agricultural University, Kunming, China. Zhulingyun_ZYS@163.com.
FAU - Li, Wengui
AU  - Li W
AD  - Department of Veterinary Medicine, College of Animal Science and Technology, 
      Yunnan Agricultural University, Kunming, China. wenguili@yeah.net.
FAU - Bi, Junlong
AU  - Bi J
AD  - Present address: Center for Animal Disease Control and Prevention of Chuxiong 
      City, Chuxiong City, Yunnan province, China. junlongbi@aliyun.com.
FAU - Yang, Guishu
AU  - Yang G
AD  - Department of Veterinary Medicine, College of Animal Science and Technology, 
      Yunnan Agricultural University, Kunming, China. 2442739714@qq.com.
FAU - Yin, Gefen
AU  - Yin G
AD  - Department of Veterinary Medicine, College of Animal Science and Technology, 
      Yunnan Agricultural University, Kunming, China. yingefen@ynau.edu.cn.
FAU - Liu, Jianping
AU  - Liu J
AD  - Present address: Karolinska Institute, Department of Biosciences and Nutrition, 
      Novum, Huddinge, Sweden. Jianping.liu@ki.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150812
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Antiviral Agents)
RN  - 0 (Oligoribonucleotides, Antisense)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/pharmacology
MH  - Cell Line
MH  - Gene Silencing
MH  - Haplorhini
MH  - Macrophages/virology
MH  - Oligoribonucleotides, Antisense/*physiology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Pulmonary Alveoli/cytology
MH  - RNA, Viral
MH  - Swine
MH  - Virus Replication
PMC - PMC4534064
EDAT- 2015/08/13 06:00
MHDA- 2016/03/22 06:00
PMCR- 2015/08/12
CRDT- 2015/08/13 06:00
PHST- 2015/01/21 00:00 [received]
PHST- 2015/07/30 00:00 [accepted]
PHST- 2015/08/13 06:00 [entrez]
PHST- 2015/08/13 06:00 [pubmed]
PHST- 2016/03/22 06:00 [medline]
PHST- 2015/08/12 00:00 [pmc-release]
AID - 10.1186/s12917-015-0518-2 [pii]
AID - 518 [pii]
AID - 10.1186/s12917-015-0518-2 [doi]
PST - epublish
SO  - BMC Vet Res. 2015 Aug 12;11:199. doi: 10.1186/s12917-015-0518-2.