PMID- 26272054 OWN - NLM STAT- MEDLINE DCOM- 20151030 LR - 20230715 IS - 1473-5644 (Electronic) IS - 0022-2615 (Print) IS - 0022-2615 (Linking) VI - 64 IP - 8 DP - 2015 Aug TI - Genetic characterization of three qnrS1-harbouring multidrug-resistance plasmids and qnrS1-containing transposons circulating in Ho Chi Minh City, Vietnam. PG - 869-878 LID - 10.1099/jmm.0.000100 [doi] AB - Plasmid-mediated quinolone resistance (PMQR) refers to a family of closely related genes that confer decreased susceptibility to fluoroquinolones. PMQR genes are generally associated with integrons and/or plasmids that carry additional antimicrobial resistance genes active against a range of antimicrobials. In Ho Chi Minh City (HCMC), Vietnam, we have previously shown a high frequency of PMQR genes within commensal Enterobacteriaceae. However, there are limited available sequence data detailing the genetic context in which the PMQR genes reside, and a lack of understanding of how these genes spread across the Enterobacteriaceae. Here, we aimed to determine the genetic background facilitating the spread and maintenance of qnrS1, the dominant PMQR gene circulating in HCMC. We sequenced three qnrS1-carrying plasmids in their entirety to understand the genetic context of these qnrS1-embedded plasmids and also the association of qnrS1-mediated quinolone resistance with other antimicrobial resistance phenotypes. Annotation of the three qnrS1-containing plasmids revealed a qnrS1-containing transposon with a closely related structure. We screened 112 qnrS1-positive commensal Enterobacteriaceae isolated in the community and in a hospital in HCMC to detect the common transposon structure. We found the same transposon structure to be present in 71.4 % (45/63) of qnrS1-positive hospital isolates and in 36.7 % (18/49) of qnrS1-positive isolates from the community. The resulting sequence analysis of the qnrS1 environment suggested that qnrS1 genes are widely distributed and are mobilized on elements with a common genetic background. Our data add additional insight into mechanisms that facilitate resistance to multiple antimicrobials in Gram-negative bacteria in Vietnam. FAU - Le, Vien AU - Le V AD - Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam. AD - Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, CA, USA. FAU - Nhu, Nguyen Thi Khanh AU - Nhu NTK AD - Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam. AD - School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia. FAU - Cerdeno-Tarraga, Ana AU - Cerdeno-Tarraga A AD - EMBL-European Bioinformatics Institute, Hinxton, Cambridge, UK. FAU - Campbell, James I AU - Campbell JI AD - Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam. AD - Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, UK. FAU - Tuyen, Ha Thanh AU - Tuyen HT AD - Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam. FAU - Nhu, Tran Do Hoang AU - Nhu TDH AD - Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam. FAU - Tam, Pham Thi Thanh AU - Tam PTT AD - Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam. FAU - Schultsz, Constance AU - Schultsz C AD - Department of Medical Microbiology, Academic Medical Centre, University of Amsterdam, The Netherlands. FAU - Thwaites, Guy AU - Thwaites G AD - Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam. AD - Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, UK. FAU - Thomson, Nicholas R AU - Thomson NR AD - Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK. AD - London School of Hygiene and Tropical Medicine, London, UK. FAU - Baker, Stephen AU - Baker S AD - Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, UK. AD - Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam. AD - London School of Hygiene and Tropical Medicine, London, UK. LA - eng SI - GENBANK/HF545433 SI - GENBANK/HF545434 SI - GENBANK/HF545435 GR - 100087/WT_/Wellcome Trust/United Kingdom GR - 089276/2/09/2/WT_/Wellcome Trust/United Kingdom GR - WT_/Wellcome Trust/United Kingdom GR - 089276/WT_/Wellcome Trust/United Kingdom GR - 100087/Z/12/Z/WT_/Wellcome Trust/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - J Med Microbiol JT - Journal of medical microbiology JID - 0224131 RN - 0 (Anti-Bacterial Agents) RN - 0 (DNA Transposable Elements) RN - 0 (DNA, Bacterial) RN - 0 (Quinolones) SB - IM MH - Anti-Bacterial Agents/pharmacology MH - Community-Acquired Infections/microbiology MH - Cross Infection/microbiology MH - *DNA Transposable Elements MH - DNA, Bacterial/chemistry/genetics MH - *Drug Resistance, Multiple, Bacterial MH - Enterobacteriaceae/drug effects/*genetics/isolation & purification MH - Enterobacteriaceae Infections/microbiology MH - Humans MH - Interspersed Repetitive Sequences MH - Molecular Sequence Data MH - *Plasmids MH - Quinolones/pharmacology MH - Sequence Analysis, DNA MH - Vietnam PMC - PMC4635468 EDAT- 2015/08/15 06:00 MHDA- 2015/10/31 06:00 PMCR- 2015/08/01 CRDT- 2015/08/15 06:00 PHST- 2015/08/15 06:00 [entrez] PHST- 2015/08/15 06:00 [pubmed] PHST- 2015/10/31 06:00 [medline] PHST- 2015/08/01 00:00 [pmc-release] AID - 000100 [pii] AID - 10.1099/jmm.0.000100 [doi] PST - ppublish SO - J Med Microbiol. 2015 Aug;64(8):869-878. doi: 10.1099/jmm.0.000100.