PMID- 26273754
OWN - NLM
STAT- MEDLINE
DCOM- 20160706
LR  - 20181202
IS  - 1532-4109 (Electronic)
IS  - 0360-1234 (Linking)
VI  - 50
IP  - 10
DP  - 2015
TI  - The effect of thiacloprid formulation on DNA/chromosome damage and changes in GST 
      activity in bovine peripheral lymphocytes.
PG  - 698-707
LID - 10.1080/03601234.2015.1048102 [doi]
AB  - The potential genotoxic effect of thiacloprid formulation on bovine peripheral 
      lymphocytes was evaluated using the comet assay and the cytogenetic endpoints: 
      chromosome aberrations (CAs), sister chromatid exchanges (SCEs) and micronuclei 
      (MNi). Whole blood cultures were treated with the insecticide at concentrations 
      of 30, 60, 120, 240 and 480 mug mL(-1) for 24, 48 h and/or 2 h of incubation. A 
      statistically significant increase in the frequency of DNA damage, as well as in 
      unstable chromosome aberrations (% breaks) were found after exposure to the 
      insecticide at concentrations ranging from 120 to 480 mug mL(-1) (P < 0.05, P < 
      0.01, P < 0.001). For the detection of stable structural chromosome aberrations 
      (e.g., translocations) and numerical aberrations by the FISH method, three whole 
      chromosome painting probes for bovine chromosomes 1, 5 and 7 (BTA1, BTA5 and 
      BTA7) were used in our experiments. We observed numerical aberrations, but 
      without any statistical significance. Regarding the sister chromatid exchanges, 
      no significant elevation in the SCE frequencies was found after 24-h exposure to 
      the insecticide. A dose-related response in the SCE induction was obtained in 
      bovine cultures after the prolonged time of exposure (48 h) to thiacloprid 
      formulation at concentrations ranging from 120 to 480 mug mL(-1) in each donor (P 
      < 0.05, P < 0.01), which was associated with a reduction of the PI (P < 0.05, P < 
      0.01). The insecticide failed to produce MNi; however, a significant reduction of 
      CBPI was observed. Using real-time PCR, a decrease in the expression of bovine 
      glutathione S-transferase M3 (GSTM3) was detected at the lowest dose. The higher 
      concentrations of thiacloprid formulation caused an increase in the mRNA 
      expression.
FAU - Galdikova, Martina
AU  - Galdikova M
AD  - a Department of Biology and Genetics , University of Veterinary Medicine and 
      Pharmacy , Kosice , Slovak Republic.
FAU - Sivikova, Katarina
AU  - Sivikova K
FAU - Holeckova, Beata
AU  - Holeckova B
FAU - Dianovsky, Jan
AU  - Dianovsky J
FAU - Drazovska, Monika
AU  - Drazovska M
FAU - Schwarzbacherova, Viera
AU  - Schwarzbacherova V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Environ Sci Health B
JT  - Journal of environmental science and health. Part. B, Pesticides, food 
      contaminants, and agricultural wastes
JID - 7607167
RN  - 0 (Insecticides)
RN  - 0 (Neonicotinoids)
RN  - 0 (Pyridines)
RN  - 0 (Thiazines)
RN  - DSV3A944A4 (thiacloprid)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured/drug effects
MH  - Chromosome Aberrations/*chemically induced
MH  - DNA Damage/*drug effects
MH  - Glutathione Transferase/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Insecticides/*toxicity
MH  - Lymphocytes/*drug effects
MH  - Neonicotinoids
MH  - Pyridines/*toxicity
MH  - Real-Time Polymerase Chain Reaction
MH  - Sister Chromatid Exchange/*drug effects
MH  - Thiazines/*toxicity
OTO - NOTNLM
OT  - Thiacloprid
OT  - bovine peripheral lymphocytes
OT  - chromosome aberrations
OT  - fluorescence in situ hybridization
OT  - glutathione s-transferases
OT  - real-time PCR
EDAT- 2015/08/15 06:00
MHDA- 2016/07/07 06:00
CRDT- 2015/08/15 06:00
PHST- 2015/08/15 06:00 [entrez]
PHST- 2015/08/15 06:00 [pubmed]
PHST- 2016/07/07 06:00 [medline]
AID - 10.1080/03601234.2015.1048102 [doi]
PST - ppublish
SO  - J Environ Sci Health B. 2015;50(10):698-707. doi: 10.1080/03601234.2015.1048102.