PMID- 26276010
OWN - NLM
STAT- MEDLINE
DCOM- 20160321
LR  - 20181202
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 17
IP  - 9
DP  - 2015 Sep
TI  - Interaction between human mature adipocytes and lymphocytes induces T-cell 
      proliferation.
PG  - 1292-301
LID - S1465-3249(15)00937-8 [pii]
LID - 10.1016/j.jcyt.2015.06.007 [doi]
AB  - BACKGROUND AIMS: Adipose tissue is a critical organ that plays a major role in 
      energy balance regulation and the immune response through intricate signals. 
      METHODS: We report on the inter-relation between mature adipocytes and 
      lymphocytes in terms of adipocyte-derived T-cell chemo-attractants and adipocyte 
      metabolic effects on lymphocytes. RESULTS: During the culture time, mature 
      adipocytes changed their structural and functional properties into 
      de-differentiated cells. Isolated mature adipocytes expressed significantly 
      higher levels of CIITA, major histocompatibility complex II (human leukocyte 
      antigen [HLA]-DR) and costimulatory signal molecule CD80 compared with adipocytes 
      after the de-differentiation process. Moreover, human leukocyte antigen-G, which 
      may prevent the immune responses of mesenchymal stromal cells, was expressed at 
      lower level in mature adipocytes compared with de-differentiated adipocytes. In 
      line with these molecular data, functional results showed different 
      immunoregulatory properties between adipocytes before and after the 
      de-differentiation process. Mature adipocytes stimulated the proliferation of 
      total lymphocytes and immunoselected cell populations CD3+, CD4+ and CD8+ in a 
      direct contact-dependent way that involved the major histocompatibility complex I 
      and II pathways. Moreover, adipocytes secreted potential chemo-attractant 
      factors, but data showed that adipocyte-derived culture medium was not sufficient 
      to activate lymphocyte proliferation, suggesting that a direct contact between 
      adipocytes and immune cells was needed. However, specific mature adipocyte 
      cytokines enhanced lymphocyte proliferation in a mixed lymphocyte reaction. 
      CONCLUSIONS: In conclusion, cross-talk occurs between adipocytes and lymphocytes 
      within adipose tissue involving T-cell chemo-attraction by mature adipocytes. Our 
      findings, together with current observations in the field, provide a rationale to 
      identify adipocyte-lymphocyte cross-talk that instigates adipose inflammation.
CI  - Copyright (c) 2015 International Society for Cellular Therapy. Published by 
      Elsevier Inc. All rights reserved.
FAU - Poloni, Antonella
AU  - Poloni A
AD  - Clinica di Ematologia, Dipartimento di Scienze Cliniche e Molecolari, Universita 
      Politecnica delle Marche, Ancona, Italy. Electronic address: a.poloni@univpm.it.
FAU - Maurizi, Giulia
AU  - Maurizi G
AD  - Clinica di Ematologia, Dipartimento di Scienze Cliniche e Molecolari, Universita 
      Politecnica delle Marche, Ancona, Italy.
FAU - Ciarlantini, Marco
AU  - Ciarlantini M
AD  - Clinica di Ematologia, Dipartimento di Scienze Cliniche e Molecolari, Universita 
      Politecnica delle Marche, Ancona, Italy.
FAU - Medici, Martina
AU  - Medici M
AD  - Clinica di Ematologia, Dipartimento di Scienze Cliniche e Molecolari, Universita 
      Politecnica delle Marche, Ancona, Italy.
FAU - Mattiucci, Domenico
AU  - Mattiucci D
AD  - Clinica di Ematologia, Dipartimento di Scienze Cliniche e Molecolari, Universita 
      Politecnica delle Marche, Ancona, Italy.
FAU - Mancini, Stefania
AU  - Mancini S
AD  - Clinica di Ematologia, Dipartimento di Scienze Cliniche e Molecolari, Universita 
      Politecnica delle Marche, Ancona, Italy.
FAU - Maurizi, Angela
AU  - Maurizi A
AD  - Clinica Chirurgia del Pancreas, Universita Politecnica delle Marche, Ospedali 
      Riuniti, Ancona, Italy.
FAU - Falconi, Massimo
AU  - Falconi M
AD  - Clinica Chirurgia del Pancreas, Universita Politecnica delle Marche, Ospedali 
      Riuniti, Ancona, Italy.
FAU - Olivieri, Attilio
AU  - Olivieri A
AD  - Clinica di Ematologia, Dipartimento di Scienze Cliniche e Molecolari, Universita 
      Politecnica delle Marche, Ancona, Italy.
FAU - Leoni, Pietro
AU  - Leoni P
AD  - Clinica di Ematologia, Dipartimento di Scienze Cliniche e Molecolari, Universita 
      Politecnica delle Marche, Ancona, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (B7-1 Antigen)
RN  - 0 (Cytokines)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (MHC class II transactivator protein)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Trans-Activators)
SB  - IM
MH  - Adipocytes/cytology/*immunology
MH  - Adipose Tissue/cytology/*immunology/physiology
MH  - Aged
MH  - Aged, 80 and over
MH  - B7-1 Antigen/immunology
MH  - Cell Communication/*immunology
MH  - Cell Differentiation/immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - HLA-DR Antigens/immunology
MH  - HLA-G Antigens/biosynthesis
MH  - Histocompatibility Antigens Class I/metabolism
MH  - Humans
MH  - Inflammation/*immunology
MH  - Lymphocyte Activation/immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Mesenchymal Stem Cells/immunology
MH  - Middle Aged
MH  - Nuclear Proteins/immunology
MH  - T-Lymphocytes/cytology/*immunology
MH  - Trans-Activators/immunology
OTO - NOTNLM
OT  - T-cell activation
OT  - de-differentiated adipocytes
OT  - inflammation
OT  - mature adipocytes
EDAT- 2015/08/16 06:00
MHDA- 2016/03/22 06:00
CRDT- 2015/08/16 06:00
PHST- 2015/02/09 00:00 [received]
PHST- 2015/05/28 00:00 [revised]
PHST- 2015/06/14 00:00 [accepted]
PHST- 2015/08/16 06:00 [entrez]
PHST- 2015/08/16 06:00 [pubmed]
PHST- 2016/03/22 06:00 [medline]
AID - S1465-3249(15)00937-8 [pii]
AID - 10.1016/j.jcyt.2015.06.007 [doi]
PST - ppublish
SO  - Cytotherapy. 2015 Sep;17(9):1292-301. doi: 10.1016/j.jcyt.2015.06.007.