PMID- 26276065
OWN - NLM
STAT- MEDLINE
DCOM- 20160524
LR  - 20191210
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1001
DP  - 2015 Sep 15
TI  - Pretreatment of plasma samples by a novel hollow fiber centrifugal 
      ultrafiltration technique for the determination of plasma protein binding of 
      three coumarins using acetone as protein binding releasing agent.
PG  - 114-23
LID - S1570-0232(15)30115-X [pii]
LID - 10.1016/j.jchromb.2015.07.048 [doi]
AB  - A novel and practical sample pretreatment method based on hollow fiber 
      centrifugal ultrafiltration (HFCF-UF) was developed to determine plasma protein 
      binding by using HPLC. The samples for analyzing unbound and total concentrations 
      could be prepared in parallel simultaneously by the same device. It only required 
      centrifugation for a short time and the filtrate could be injected directly for 
      HPLC analysis without further treatment. Coumarins were selected as the model 
      drugs. Acetone was chosen as the releasing agent to free the binding drug from 
      the drug-protein complex for the total drug concentration determination. 
      Non-specific bindings (NSBs) between the analytes and hollow fiber membrane 
      materials were investigated. The type and volume of protein binding releaser were 
      optimized. Additionally, centrifugal speed and centrifugal time were considered. 
      Under the optimized conditions, the absolute recovery rates of the unbound and 
      total concentrations were in the range of 97.5-100.9% for the three analytes. The 
      limits of detection were in the range of 0.0135-0.0667mugmL(-1). In vitro plasma 
      protein binding of the three coumarins was determined at three concentrations 
      using the validated method and the relative standard deviations (RSDs) were less 
      than 3.4%. Compared with traditional method, the HFCF-UF method is simple to run, 
      no specialized equipment requirement and is a more accurate plasma pretreatment 
      procedure with almost excellent drug-protein binding equilibrium. Therefore, this 
      method can be applied to determine the plasma protein binding in clinical 
      practice. It also provides a reliable alternative for accurate monitoring of 
      unbound or total drug concentration in therapeutic drug monitoring (TDM).
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Li, Junmei
AU  - Li J
AD  - School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road, 
      Shijiazhuang 050017, PR China.
FAU - Shi, Qingwen
AU  - Shi Q
AD  - School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road, 
      Shijiazhuang 050017, PR China. Electronic address: shiqingwen11@163.com.
FAU - Jiang, Ye
AU  - Jiang Y
AD  - School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road, 
      Shijiazhuang 050017, PR China. Electronic address: jiangye@hebmu.edu.cn.
FAU - Liu, Yan
AU  - Liu Y
AD  - School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road, 
      Shijiazhuang 050017, PR China.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20150731
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Blood Proteins)
RN  - 0 (Coumarins)
RN  - 1364PS73AF (Acetone)
SB  - IM
MH  - Acetone/*metabolism
MH  - Blood Proteins/*metabolism
MH  - Centrifugation
MH  - Chromatography, High Pressure Liquid
MH  - Coumarins/*metabolism
MH  - Humans
MH  - Protein Binding
MH  - Ultrafiltration/*methods
OTO - NOTNLM
OT  - Acetone
OT  - Coumarins
OT  - HPLC
OT  - Hollow fiber centrifugal ultrafiltration
OT  - Plasma protein binding
OT  - Protein binding displacer
EDAT- 2015/08/16 06:00
MHDA- 2016/05/25 06:00
CRDT- 2015/08/16 06:00
PHST- 2015/05/02 00:00 [received]
PHST- 2015/07/20 00:00 [revised]
PHST- 2015/07/26 00:00 [accepted]
PHST- 2015/08/16 06:00 [entrez]
PHST- 2015/08/16 06:00 [pubmed]
PHST- 2016/05/25 06:00 [medline]
AID - S1570-0232(15)30115-X [pii]
AID - 10.1016/j.jchromb.2015.07.048 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Sep 15;1001:114-23. doi: 
      10.1016/j.jchromb.2015.07.048. Epub 2015 Jul 31.