PMID- 26276126
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181113
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 39
IP  - 1
DP  - 2016 Feb
TI  - Probenecid Application Prevents Clinical Symptoms and Inflammation in 
      Experimental Autoimmune Encephalomyelitis.
PG  - 123-128
LID - 10.1007/s10753-015-0230-1 [doi]
AB  - Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous 
      system. Neurological impairments are caused by axonal damage due to demyelination 
      and neuroinflammation within the central nervous system. T cells mediate the 
      neuroinflammation. The activation of T cells is induced by the release of 
      adenosine triphosphate and involves purinergic receptors as well as pannexin 
      (Panx) proteins. As Panx1 is expressed on T cells, we here propose that 
      application of probenecid, a known Panx inhibitor, will prevent the onset of 
      clinical symptoms in a mouse model of MS, the experimental autoimmune 
      encephalomyelitis (EAE) model. EAE-induced mice received daily injections of 
      probenecid. Disease scores, T cell numbers, and microglia activation were 
      compared between experimental groups. Probenecid treatment resulted in lower 
      disease scores as compared to EAE animals. Probenecid-treated animals also 
      displayed fewer inflammatory lesions. Microglia activation was not altered by 
      treatment. In conclusion, probenecid prevented the onset of EAE.
FAU - Hainz, Nadine
AU  - Hainz N
AD  - Department of Anatomy and Cell Biology, Saarland University, Building 61, 66421, 
      Homburg, Saar, Germany.
FAU - Wolf, Sandra
AU  - Wolf S
AD  - Department of Anatomy and Cell Biology, Saarland University, Building 61, 66421, 
      Homburg, Saar, Germany.
FAU - Tschernig, Thomas
AU  - Tschernig T
AD  - Department of Anatomy and Cell Biology, Saarland University, Building 61, 66421, 
      Homburg, Saar, Germany.
FAU - Meier, Carola
AU  - Meier C
AD  - Department of Anatomy and Cell Biology, Saarland University, Building 61, 66421, 
      Homburg, Saar, Germany. Carola.Meier@uks.eu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Adjuvants, Pharmaceutic)
RN  - 0 (Connexins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Panx1 protein, mouse)
RN  - PO572Z7917 (Probenecid)
SB  - IM
MH  - Adjuvants, Pharmaceutic/*therapeutic use
MH  - Animals
MH  - Central Nervous System/immunology/pathology
MH  - Connexins/*antagonists & inhibitors
MH  - Disease Models, Animal
MH  - Encephalomyelitis, Autoimmune, Experimental/*drug therapy/immunology/prevention & 
      control
MH  - Female
MH  - Inflammation/drug therapy/*prevention & control
MH  - Lymphocyte Activation/immunology
MH  - Lymphocyte Count
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microglia/immunology
MH  - Multiple Sclerosis/immunology
MH  - Nerve Tissue Proteins/*antagonists & inhibitors
MH  - Probenecid/*therapeutic use
OTO - NOTNLM
OT  - multiple sclerosis
OT  - neuroinflammation
OT  - pannexin
OT  - probenecid
EDAT- 2015/08/16 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/08/16 06:00
PHST- 2015/08/16 06:00 [entrez]
PHST- 2015/08/16 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1007/s10753-015-0230-1 [pii]
AID - 10.1007/s10753-015-0230-1 [doi]
PST - ppublish
SO  - Inflammation. 2016 Feb;39(1):123-128. doi: 10.1007/s10753-015-0230-1.