PMID- 26277502
OWN - NLM
STAT- MEDLINE
DCOM- 20150925
LR  - 20181202
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 35
IP  - 8
DP  - 2015 Aug
TI  - [Optimization of the protocols for in vitro culture and induction of hepatic 
      differentiation of rat mesenchymal stem cells].
PG  - 1090-6
AB  - OBJECTIVE: To optimize the protocols for isolation, in vitro culture, 
      identification and induction of hepatic differentiation of rat bone marrow 
      mesenchymal stem cells (BMSCs). METHODS: Rat BMSCs were separated and purified by 
      differential adherent culture for 1.5 h with the first medium change at 12 h. The 
      surface markers of BMSCs were detected by flow cytometry. The cells were induced 
      to differentiate into adipogenic, osteogenic, and chondrogenesis lineages. A 
      3-step protocol including sequential addition of growth factors, cytokines and 
      hormones was used to induce the BMSCs to differentiate into hepatocyte-like 
      cells. RESULTS: The cells isolated using this protocol were positive for CD29, 
      CD44, and CD90 and negative for CD29 and CD45. The adipogenic, osteogenic, and 
      chondrogenic differentiation of the BMSCs were verified by Oil red, Alizarin red, 
      and toluidine blue staining. The BMSCs induced with the 3-step protocol 
      differentiated into hepatic-like cells that expressed hepatocyte-specific 
      proteins (ALB and AFP) and genes. CONCLUSION: The optimized protocol allows 
      simple and efficient isolation of highly purified populations of BMSCs, which can 
      be induced into hepatic lineages in specific microenvironment.
FAU - Xiang, Junxi
AU  - Xiang J
AD  - Department of Hepatobiliary Surgery, First Affiliated Hospital1, Research 
      Institute of Advanced Surgical Technology and Engineering2, Xi'an Jiaotong 
      University, Xi'an 710061, China. E-mail: xjx722@163.com.
FAU - Zheng, Xinglong
AU  - Zheng X
FAU - Zhu, Xulong
AU  - Zhu X
FAU - Yang, Lifei
AU  - Yang L
FAU - Gao, Rui
AU  - Gao R
FAU - Li, Jianhui
AU  - Li J
FAU - Liu, Xuemin
AU  - Liu X
FAU - Lv, Yi
AU  - Lv Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
SB  - IM
MH  - Animals
MH  - *Cell Culture Techniques
MH  - *Cell Differentiation
MH  - Flow Cytometry
MH  - Hepatocytes/*cytology
MH  - Mesenchymal Stem Cells/*cytology
MH  - Rats
EDAT- 2015/08/19 06:00
MHDA- 2015/09/26 06:00
CRDT- 2015/08/17 06:00
PHST- 2015/08/17 06:00 [entrez]
PHST- 2015/08/19 06:00 [pubmed]
PHST- 2015/09/26 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2015 Aug;35(8):1090-6.