PMID- 26278282
OWN - NLM
STAT- MEDLINE
DCOM- 20160329
LR  - 20150904
IS  - 1423-0259 (Electronic)
IS  - 0030-3747 (Linking)
VI  - 54
IP  - 2
DP  - 2015
TI  - Aralia elata prevents neuronal death by downregulating tonicity response element 
      binding protein in diabetic retinopathy.
PG  - 85-95
LID - 10.1159/000437356 [doi]
AB  - BACKGROUND/AIMS: The present study addresses the role of tonicity response 
      element binding protein (TonEBP) in retinal ganglion cell (RGC) death in diabetic 
      retinopathy and the impact of Aralia elata extract on the TonEBP/RGC interaction. 
      METHODS: Diabetes was induced in C57BL/6 mice by intraperitoneal injection of 
      streptozotocin (STZ). Control mice received phosphate-buffered saline. After five 
      injections of STZ or saline buffer, A. elata extract was administered by daily 
      oral tube feeding for 7 weeks. All mice were killed at 2 months after the last 
      injection of STZ or saline and the extent of cell death together with the protein 
      expression levels of TonEBP, aldose reductase (AR) and nuclear factor-kappa B 
      (NF-kappaB) were examined. RESULTS: Terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL)-positive signals were colocalized with 
      TonEBP-immunoreactive RGCs. The apoptotic cell death of RGCs and the expression 
      levels of TonEBP, AR and NF-kappaB were significantly increased in the retinas of 
      diabetic mice compared with controls at 2 months after the induction of diabetes. 
      However, these changes were effectively blocked by the administration of A. elata 
      extract. CONCLUSIONS: These results indicate that A. elata prevents 
      diabetes-induced RGC apoptosis and downregulates TonEBP expression. Therefore, A. 
      elata extract may have therapeutic potential to prevent diabetes-induced retinal 
      neurodegeneration in diabetic retinopathy.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Kim, Seong-Jae
AU  - Kim SJ
AD  - Department of Ophthalmology, School of Medicine, Gyeongsang National University, 
      Jinju, Korea.
FAU - Yoo, Woong-Sun
AU  - Yoo WS
FAU - Kim, Hwajin
AU  - Kim H
FAU - Kwon, Jeong Eun
AU  - Kwon JE
FAU - Hong, Eun-Kyung
AU  - Hong EK
FAU - Choi, Meeyoung
AU  - Choi M
FAU - Han, Yongseop
AU  - Han Y
FAU - Chung, Inyoung
AU  - Chung I
FAU - Seo, Seongwook
AU  - Seo S
FAU - Park, Jongmoon
AU  - Park J
FAU - Yoo, Ji-Myong
AU  - Yoo JM
FAU - Choi, Wan-Sung
AU  - Choi WS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150806
PL  - Switzerland
TA  - Ophthalmic Res
JT  - Ophthalmic research
JID - 0267442
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - *Aralia
MH  - Diabetes Mellitus, Experimental/*complications
MH  - Diabetic Retinopathy/drug therapy/*metabolism
MH  - Down-Regulation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NFATC Transcription Factors/*metabolism
MH  - Plant Extracts/*pharmacology
MH  - Retina/drug effects/metabolism
MH  - Retinal Ganglion Cells/*drug effects/metabolism
EDAT- 2015/08/19 06:00
MHDA- 2016/03/30 06:00
CRDT- 2015/08/18 06:00
PHST- 2015/01/22 00:00 [received]
PHST- 2015/07/02 00:00 [accepted]
PHST- 2015/08/18 06:00 [entrez]
PHST- 2015/08/19 06:00 [pubmed]
PHST- 2016/03/30 06:00 [medline]
AID - 000437356 [pii]
AID - 10.1159/000437356 [doi]
PST - ppublish
SO  - Ophthalmic Res. 2015;54(2):85-95. doi: 10.1159/000437356. Epub 2015 Aug 6.