PMID- 26278433
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150819
LR  - 20150817
IS  - 1948-7185 (Electronic)
IS  - 1948-7185 (Linking)
VI  - 5
IP  - 19
DP  - 2014 Oct 2
TI  - Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic 
      Nuclear Polarization.
PG  - 3290-5
LID - 10.1021/jz501411d [doi]
AB  - Dissolution dynamic nuclear polarization (D-DNP) experiments enabled us to study 
      the kinetics of the enzymatic phosphorylation reaction of glucose to form 
      glucose-6-phosphate (G6P) by hexokinase (HK), with or without the presence of an 
      excess of G6P, which is known to be an inhibitor of the enzyme. Against all 
      expectations, our observations demonstrate that the phosphorylation of both alpha and 
      beta glucose anomers occurs with comparable kinetics. The catalytic constant of the 
      reaction was estimated based on a simple kinetic model tailored for 
      hyperpolarized systems.
FAU - Miclet, Emeric
AU  - Miclet E
AD  - daggerEcole Normale Superieure-PSL Research University, Departement de Chimie, 24 rue 
      Lhomond, F-75005 Paris, France.
AD  - double daggerSorbonne Universites, UPMC Univ Paris 06, LBM, 4 place Jussieu, F-75005, Paris, 
      France.
AD  -  section signCNRS, UMR 7203 LBM, F-75005, Paris, France.
FAU - Abergel, Daniel
AU  - Abergel D
AD  - daggerEcole Normale Superieure-PSL Research University, Departement de Chimie, 24 rue 
      Lhomond, F-75005 Paris, France.
AD  - double daggerSorbonne Universites, UPMC Univ Paris 06, LBM, 4 place Jussieu, F-75005, Paris, 
      France.
AD  -  section signCNRS, UMR 7203 LBM, F-75005, Paris, France.
FAU - Bornet, Aurelien
AU  - Bornet A
AD  -  parallelInstitut des Sciences et Ingenierie Chimiques, Ecole Polytechnique Federale de 
      Lausanne (EPFL), Batochime, CH-1015 Lausanne, Switzerland.
FAU - Milani, Jonas
AU  - Milani J
AD  -  parallelInstitut des Sciences et Ingenierie Chimiques, Ecole Polytechnique Federale de 
      Lausanne (EPFL), Batochime, CH-1015 Lausanne, Switzerland.
FAU - Jannin, Sami
AU  - Jannin S
AD  - daggerEcole Normale Superieure-PSL Research University, Departement de Chimie, 24 rue 
      Lhomond, F-75005 Paris, France.
AD  - double daggerSorbonne Universites, UPMC Univ Paris 06, LBM, 4 place Jussieu, F-75005, Paris, 
      France.
AD  -  section signCNRS, UMR 7203 LBM, F-75005, Paris, France.
AD  -  parallelInstitut des Sciences et Ingenierie Chimiques, Ecole Polytechnique Federale de 
      Lausanne (EPFL), Batochime, CH-1015 Lausanne, Switzerland.
AD  -  perpendicularBruker BioSpin AG, Industriestrasse 26, 8117 Fallanden, Switzerland.
FAU - Bodenhausen, Geoffrey
AU  - Bodenhausen G
AD  - daggerEcole Normale Superieure-PSL Research University, Departement de Chimie, 24 rue 
      Lhomond, F-75005 Paris, France.
AD  - double daggerSorbonne Universites, UPMC Univ Paris 06, LBM, 4 place Jussieu, F-75005, Paris, 
      France.
AD  -  section signCNRS, UMR 7203 LBM, F-75005, Paris, France.
AD  -  parallelInstitut des Sciences et Ingenierie Chimiques, Ecole Polytechnique Federale de 
      Lausanne (EPFL), Batochime, CH-1015 Lausanne, Switzerland.
LA  - eng
PT  - Journal Article
DEP - 20140912
PL  - United States
TA  - J Phys Chem Lett
JT  - The journal of physical chemistry letters
JID - 101526034
OTO - NOTNLM
OT  - dissolution dynamic nuclear polarization
OT  - enzyme kinetics
OT  - glucose-6-phosphate
OT  - hexokinase
OT  - hyperpolarization
OT  - nuclear magnetic resonance
EDAT- 2015/08/19 06:00
MHDA- 2015/08/19 06:01
CRDT- 2015/08/18 06:00
PHST- 2015/08/18 06:00 [entrez]
PHST- 2015/08/19 06:00 [pubmed]
PHST- 2015/08/19 06:01 [medline]
AID - 10.1021/jz501411d [doi]
PST - ppublish
SO  - J Phys Chem Lett. 2014 Oct 2;5(19):3290-5. doi: 10.1021/jz501411d. Epub 2014 Sep 
      12.