PMID- 26282437
OWN - NLM
STAT- MEDLINE
DCOM- 20160801
LR  - 20221207
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 22
IP  - 24
DP  - 2015 Dec
TI  - Nitrifying bacterial biomass and nitrification activity evaluated by FISH and an 
      automatic on-line instrument at full-scale Fusina (Venice, Italy) WWTP.
PG  - 19786-92
LID - 10.1007/s11356-015-5140-0 [doi]
AB  - In this study, monthly variations in biomass of ammonia-oxidizing bacteria (AOB) 
      and nitrite-oxidizing bacteria (NOB) were analysed over a 1-year period by 
      fluorescence in situ hybridization (FISH) at the full-scale Fusina WWTP. The 
      nitrification capacity of the plant was also monitored using periodic 
      respirometric batch tests and by an automated on-line titrimetric instrument 
      (TITrimetric Automated ANalyser). The percentage of nitrifying bacteria in the 
      plant was the highest in summer and was in the range of 10-15 % of the active 
      biomass. The maximum nitrosation rate varied in the range 2.0-4.0 mg NH4 g(-1) 
      VSS h(-1) (0.048-0.096 kg TKN kg(-1) VSS day(-1)): values obtained by laboratory 
      measurements and the on-line instrument were similar and significantly 
      correlated. The activity measurements provided a valuable tool for estimating the 
      maximum total Kjeldahl nitrogen (TKN) loading possible at the plant and provided 
      an early warning of whether the TKN was approaching its limiting value. The FISH 
      analysis permitted determination of the nitrifying biomass present. The main 
      operational parameter affecting both the population dynamics and the maximum 
      nitrosation activity was mixed liquor volatile suspended solids (MLVSS) 
      concentration and was negatively correlated with ammonia-oxidizing bacteria (AOB) 
      (p = 0.029) and (NOB) (p = 0.01) abundances and positively correlated with 
      maximum nitrosation rates (p = 0.035). Increases in concentrations led to 
      decreases in nitrifying bacteria abundance, but their nitrosation activity was 
      higher. These results demonstrate the importance of MLVSS concentration as key 
      factor in the development and activity of nitrifying communities in wastewater 
      treatment plants (WWTPs). Operational data on VSS and sludge volume index (SVI) 
      values are also presented on 11-year basis observations.
FAU - Badoer, S
AU  - Badoer S
AD  - VERITAS, Venice, Italy.
FAU - Miana, P
AU  - Miana P
AD  - VERITAS, Venice, Italy.
FAU - Della Sala, S
AU  - Della Sala S
AD  - VERITAS, Venice, Italy.
FAU - Marchiori, G
AU  - Marchiori G
AD  - VERITAS, Venice, Italy.
FAU - Tandoi, V
AU  - Tandoi V
AD  - Water Research Institute-National Research Council (IRSA-CNR), Via Salaria km 
      29.300-CP10, 00015, Monterotondo, Roma, Italy.
FAU - Di Pippo, F
AU  - Di Pippo F
AD  - Water Research Institute-National Research Council (IRSA-CNR), Via Salaria km 
      29.300-CP10, 00015, Monterotondo, Roma, Italy. dipippo@irsa.cnr.it.
AD  - Institute for Coastal Marine Environment-National Research Council (CNR-IAMC), 
      Localita Sa Mardini, Torregrande, Oristano, Italy. dipippo@irsa.cnr.it.
LA  - eng
PT  - Journal Article
DEP - 20150819
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Nitrites)
RN  - 0 (Sewage)
RN  - 0 (Waste Water)
SB  - IM
MH  - Betaproteobacteria/genetics/*growth & development
MH  - Biomass
MH  - In Situ Hybridization, Fluorescence
MH  - Italy
MH  - Nitrification
MH  - Nitrites/*analysis/metabolism
MH  - Nitrobacter/genetics/*growth & development
MH  - Planctomycetales/genetics/growth & development
MH  - Seasons
MH  - Sewage/*microbiology
MH  - Verrucomicrobia/genetics/growth & development
MH  - Wastewater/*microbiology
MH  - Water Purification/*methods
OTO - NOTNLM
OT  - AOB
OT  - Automatic titrimetric systems
OT  - FISH (fluorescence in situ hybridization)
OT  - Full-scale WWTP
OT  - NOB
OT  - Nitrification
OT  - Nitrosation rate
OT  - Respirometers
EDAT- 2015/08/19 06:00
MHDA- 2016/08/02 06:00
CRDT- 2015/08/19 06:00
PHST- 2015/03/27 00:00 [received]
PHST- 2015/07/30 00:00 [accepted]
PHST- 2015/08/19 06:00 [entrez]
PHST- 2015/08/19 06:00 [pubmed]
PHST- 2016/08/02 06:00 [medline]
AID - 10.1007/s11356-015-5140-0 [pii]
AID - 10.1007/s11356-015-5140-0 [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2015 Dec;22(24):19786-92. doi: 
      10.1007/s11356-015-5140-0. Epub 2015 Aug 19.