PMID- 2628268 OWN - NLM STAT- MEDLINE DCOM- 19900426 LR - 20131121 IS - 0301-1208 (Print) IS - 0301-1208 (Linking) VI - 26 IP - 5 DP - 1989 Oct TI - Spectrophotometric and spectrofluorometric titrations of teichoic acid. PG - 311-4 AB - Spectrophotometric and spectrofluorometric titrations of two strongly aggregating dyes, 1,9-dimethylmethylene blue (DMMB) and acridine orange (AO), by three anionic biopolymers, chondroitin sulphate A, DNA and teichoic acid (TA), have been described. Though the three polymers differ in their dye binding efficiencies and TA is a weak chromotrope, the equivalent weights of the polymers can be estimated accurately by these methods using the two dyes. Results show DMMB to be the preferred dye for spectrophotometric titration. The titrations can be used to estimate the equivalent weights of anionic polymers, and also for the quantitative estimation of such polymers of known equivalent weights. FAU - Pal, M K AU - Pal MK FAU - Ghosh, J K AU - Ghosh JK FAU - Das, S AU - Das S LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - India TA - Indian J Biochem Biophys JT - Indian journal of biochemistry & biophysics JID - 0310774 RN - 0 (Teichoic Acids) RN - F30N4O6XVV (Acridine Orange) RN - T42P99266K (Methylene Blue) RN - U99510HTUM (1,9-dimethylmethylene blue) SB - IM MH - Acridine Orange MH - Methylene Blue MH - Spectrometry, Fluorescence MH - Spectrophotometry MH - Teichoic Acids/*analysis EDAT- 1989/10/01 00:00 MHDA- 1989/10/01 00:01 CRDT- 1989/10/01 00:00 PHST- 1989/10/01 00:00 [pubmed] PHST- 1989/10/01 00:01 [medline] PHST- 1989/10/01 00:00 [entrez] PST - ppublish SO - Indian J Biochem Biophys. 1989 Oct;26(5):311-4.